##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064066_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3119390
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.271820772651065	32.0	32.0	32.0	32.0	32.0
2	30.86799213948881	32.0	32.0	32.0	32.0	32.0
3	30.892500136244585	32.0	32.0	32.0	32.0	32.0
4	30.91970705811072	32.0	32.0	32.0	32.0	32.0
5	30.83579770403829	32.0	32.0	32.0	32.0	32.0
6	34.51127688426263	36.0	36.0	36.0	32.0	36.0
7	34.43840237995249	36.0	36.0	36.0	32.0	36.0
8	34.395899839391674	36.0	36.0	36.0	32.0	36.0
9	34.53432658308195	36.0	36.0	36.0	32.0	36.0
10	34.22649652656449	36.0	36.0	36.0	32.0	36.0
11	34.523701108229496	36.0	36.0	36.0	32.0	36.0
12	34.33072716140015	36.0	36.0	36.0	32.0	36.0
13	34.41284834534957	36.0	36.0	36.0	32.0	36.0
14	34.30764155812515	36.0	36.0	36.0	32.0	36.0
15	34.25063073229061	36.0	36.0	36.0	32.0	36.0
16	34.25060284222236	36.0	36.0	36.0	32.0	36.0
17	34.17593600030775	36.0	36.0	36.0	32.0	36.0
18	34.16955302158435	36.0	36.0	36.0	32.0	36.0
19	34.18523621605506	36.0	36.0	36.0	32.0	36.0
20	34.16108373752561	36.0	36.0	36.0	32.0	36.0
21	34.14555890735048	36.0	36.0	36.0	32.0	36.0
22	34.11768967650727	36.0	36.0	36.0	32.0	36.0
23	34.069183718611654	36.0	36.0	36.0	32.0	36.0
24	34.05125104587756	36.0	36.0	36.0	32.0	36.0
25	33.657369549815826	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	13.0
4	30.0
5	41.0
6	121.0
7	61.0
8	160.0
9	192.0
10	270.0
11	88.0
12	196.0
13	186.0
14	315.0
15	540.0
16	882.0
17	1162.0
18	1475.0
19	2126.0
20	3184.0
21	4728.0
22	7195.0
23	11409.0
24	16755.0
25	23803.0
26	33419.0
27	44677.0
28	60404.0
29	81361.0
30	107384.0
31	145509.0
32	206573.0
33	295128.0
34	640722.0
35	1429280.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.713511450626484	17.10859253985511	11.264780303832056	25.913115705686344
2	16.309556784927455	19.979142141210204	38.291579144449486	25.41972192941285
3	18.798578031164954	24.43939208876758	28.7071900214693	28.054839858598157
4	12.35909417615977	15.746989210978679	36.51356305713943	35.38035355572212
5	14.242514789598484	37.011058374830554	33.83422825419258	14.91219858137838
6	33.32108565710546	36.044077527149284	17.432418957735578	13.202417858009676
7	29.828160557058514	30.71969530571876	21.089138404324668	18.36300573289806
8	27.86553722830078	33.32479220690093	19.57369623820571	19.23597432659258
9	27.757259727934404	14.053686337159792	18.432631963490238	39.75642197141557
10	15.552873257571068	26.922505716812417	32.15031375508852	25.374307270528
11	36.9178723259575	20.902164381083495	23.305667266903555	18.874296026055458
12	25.125223702541298	23.51122931423122	29.43882138472795	21.924725598499528
13	29.6738081971541	19.738762987990953	25.296376883421086	25.291051931433866
14	23.448004406387206	20.044089535375726	25.61202941141561	30.895876646821456
15	24.6837010362917	27.943949276687253	22.54458915617897	24.827760530842077
16	25.14620089398278	25.75644326507096	24.643263402844653	24.454092438101604
17	23.485644117305522	26.085183861669254	25.73816368651035	24.691008334514876
18	24.333943862735794	25.143801650771408	27.078663328806496	23.443591157686303
19	25.415742722657676	24.787589122099202	25.629853914186135	24.16681424105699
20	25.719342868747635	24.651212630841382	25.310288096474043	24.319156403936937
21	26.658355346781587	24.202591309559025	24.698832287360357	24.440221056299034
22	25.708369733405494	24.829314006084736	25.1218406528625	24.34047560764727
23	24.211186580979668	24.61776133625516	25.770108156841857	25.400943925923315
24	24.719950772421264	25.10648223007605	25.69985036664023	24.47371663086246
25	24.711656858989535	24.84258630082138	25.75431207342059	24.691444766768498
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	572.0
1	572.0
2	1042.5
3	1513.0
4	1513.0
5	1513.0
6	4068.5
7	6624.0
8	6624.0
9	6624.0
10	6645.0
11	6666.0
12	6666.0
13	6666.0
14	7541.0
15	8416.0
16	8416.0
17	8416.0
18	14956.0
19	21496.0
20	21496.0
21	21496.0
22	35419.5
23	49343.0
24	49343.0
25	49343.0
26	75036.0
27	100729.0
28	100729.0
29	100729.0
30	128323.0
31	155917.0
32	155917.0
33	155917.0
34	183908.0
35	211899.0
36	211899.0
37	211899.0
38	237202.0
39	262505.0
40	262505.0
41	262505.0
42	295741.5
43	328978.0
44	328978.0
45	328978.0
46	363416.0
47	397854.0
48	397854.0
49	397854.0
50	405823.0
51	413792.0
52	413792.0
53	413792.0
54	382927.5
55	352063.0
56	352063.0
57	352063.0
58	322528.0
59	292993.0
60	292993.0
61	292993.0
62	256046.5
63	219100.0
64	219100.0
65	219100.0
66	179994.0
67	140888.0
68	140888.0
69	140888.0
70	105906.0
71	70924.0
72	70924.0
73	70924.0
74	54074.0
75	37224.0
76	37224.0
77	37224.0
78	29747.5
79	22271.0
80	22271.0
81	22271.0
82	15366.0
83	8461.0
84	8461.0
85	8461.0
86	6539.0
87	4617.0
88	4617.0
89	4617.0
90	3327.5
91	2038.0
92	2038.0
93	2038.0
94	1434.5
95	831.0
96	831.0
97	831.0
98	1253.5
99	1676.0
100	1676.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03911021065015917
2	0.00583447404781063
3	0.0016990501348019965
4	0.0034622153690304835
5	0.009809610212253036
6	0.013816803926408688
7	0.02317760844267629
8	0.03667383687195253
9	0.0493365690086844
10	0.058697373524951996
11	0.05552367610334072
12	0.06312131538537984
13	0.06382658147907123
14	0.06722468174867523
15	0.06180695584713679
16	0.06744908459666793
17	0.06385863902878447
18	0.07767544295519316
19	0.07430940023530241
20	0.07847688169802429
21	0.07552758712440573
22	0.07722663725920773
23	0.08399078024870248
24	0.07815630620089184
25	0.0783486514991713
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3119390.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.87100409427422
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.31864558304281	28.186992848285808
2	16.399301868467152	13.733104713556541
3	6.68519100628118	8.397469799850134
4	3.2980854697353226	5.523766008262161
5	1.7827437600974296	3.7322635639040644
6	1.0876966298790547	2.7325770025796507
7	0.7037951923191409	2.0628027965387687
8	0.5013151604968303	1.6792455310147618
9	0.35061771592819524	1.3212644237239086
>10	1.6703521274526905	12.119681530380173
>50	0.09891956366390113	2.8894699885486976
>100	0.08103879163856548	7.321941045613653
>500	0.013887486434767537	4.00858228836012
>1k	0.008255339158241939	5.8045248425817
>5k	1.5430540482695213E-4	0.4863136167999116
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	8727	0.27976623634749104	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6327	0.20282811703570247	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4825	0.15467767736640817	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3183	0.10203918073725952	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	3143	0.10075687874872973	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	3133	0.10043630325159726	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.411509942649043E-5
2	0.0	0.0	0.0	0.0	6.411509942649043E-5
3	0.0	0.0	0.0	0.0	6.411509942649043E-5
4	0.0	0.0	0.0	0.0	6.411509942649043E-5
5	0.0	0.0	0.0	0.0	9.617264913973566E-5
6	0.0	0.0	0.0	0.0	1.602877485662261E-4
7	0.0	0.0	0.0	3.2057549713245215E-5	1.9234529827947132E-4
8	0.0	0.0	0.0	3.2057549713245215E-5	1.9234529827947132E-4
9	0.0	0.0	0.0	6.411509942649043E-5	1.9234529827947132E-4
10	0.0	0.0	0.0	6.411509942649043E-5	1.9234529827947132E-4
11	0.0	0.0	0.0	6.411509942649043E-5	1.9234529827947132E-4
12	0.0	0.0	0.0	6.411509942649043E-5	4.4880569598543306E-4
13	0.0	0.0	0.0	9.617264913973566E-5	6.090934445516592E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCAACG	275	0.0	12.09245	18
CCAACGA	250	0.0	11.781691	19
AACGGAC	65	8.018816E-4	11.693798	15
GTATAAT	220	0.0	11.657024	1
CGAACGA	160	4.5474735E-11	11.282689	16
CCGAACC	60	0.005882856	11.08208	4
ACTCTAA	575	0.0	11.070266	10
ATCCCCG	455	0.0	11.066635	12
GTATAGG	250	0.0	11.018046	1
GCGTAAC	165	8.0035534E-11	10.939384	11
ATTTAGA	480	0.0	10.883487	1
ACGGTAT	375	0.0	10.641016	9
AACCGCG	250	0.0	10.640162	7
CGGTCCA	575	0.0	10.574582	10
CGCGCTA	135	5.6834324E-8	10.556902	16
ACGCGCG	135	5.6888894E-8	10.556225	13
AATCCCC	470	0.0	10.51063	11
CCGTCGT	525	0.0	10.49624	9
GTATCAA	5625	0.0	10.36794	1
CGCCCCT	330	0.0	10.362797	5
>>END_MODULE