FastQCFastQC Report
Thu 2 Feb 2017
SRR4064066_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064066_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3119390
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA31710.10165449014070058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG16050.020.6964324
ACGATTA1750.020.11422740
TTAGGAC11550.019.998343
ATACCGT7300.019.5877276
GTATCAA65200.019.4352151
TACCGTC7300.018.6836787
GTCCTAC14950.018.5414541
ACGACTA600.0074175318.3321065
TGGCGAG19100.018.31261418
GATATAC10650.018.178061
TATGGCG19000.018.17743916
ACCGTCG7450.018.0122178
AGGACGT17850.017.7467085
CCGTCGT7500.017.6002319
GTAGGAC18150.017.4530963
GACGTGA10100.017.2067667
ATTTCGT8200.017.17013242
GGACGTG17950.016.7899596
TAATACC3550.016.7312744
CGTCGTA7800.016.64124510
Produced by FastQC (version 0.11.5)