##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064066_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3119390
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27520829392926	32.0	32.0	32.0	32.0	32.0
2	31.425664953724926	32.0	32.0	32.0	32.0	32.0
3	31.499991665037076	32.0	32.0	32.0	32.0	32.0
4	31.586163961543765	32.0	32.0	32.0	32.0	32.0
5	31.53651867833134	32.0	32.0	32.0	32.0	32.0
6	35.13137985311231	36.0	36.0	36.0	36.0	36.0
7	35.14468117163933	36.0	36.0	36.0	36.0	36.0
8	35.09736679286655	36.0	36.0	36.0	36.0	36.0
9	35.195535665626934	36.0	36.0	36.0	36.0	36.0
10	35.06484504983346	36.0	36.0	36.0	36.0	36.0
11	35.2036667425362	36.0	36.0	36.0	36.0	36.0
12	35.11204594488025	36.0	36.0	36.0	36.0	36.0
13	35.14451351065433	36.0	36.0	36.0	36.0	36.0
14	35.092944774459106	36.0	36.0	36.0	36.0	36.0
15	35.067357079428994	36.0	36.0	36.0	36.0	36.0
16	35.08180317305627	36.0	36.0	36.0	36.0	36.0
17	35.04106283600319	36.0	36.0	36.0	36.0	36.0
18	35.03896691340294	36.0	36.0	36.0	36.0	36.0
19	35.03944553262016	36.0	36.0	36.0	36.0	36.0
20	35.03091790382094	36.0	36.0	36.0	36.0	36.0
21	35.02353761472595	36.0	36.0	36.0	36.0	36.0
22	35.00875010819423	36.0	36.0	36.0	36.0	36.0
23	34.95730222896143	36.0	36.0	36.0	36.0	36.0
24	34.931694337675	36.0	36.0	36.0	36.0	36.0
25	34.90526737599338	36.0	36.0	36.0	32.0	36.0
26	34.84469944444266	36.0	36.0	36.0	32.0	36.0
27	34.84327737153738	36.0	36.0	36.0	32.0	36.0
28	34.81292688634637	36.0	36.0	36.0	32.0	36.0
29	34.785029124283916	36.0	36.0	36.0	32.0	36.0
30	34.76620012245984	36.0	36.0	36.0	32.0	36.0
31	34.75517713399094	36.0	36.0	36.0	32.0	36.0
32	34.71084474849249	36.0	36.0	36.0	32.0	36.0
33	34.68749851733833	36.0	36.0	36.0	32.0	36.0
34	34.67600171828467	36.0	36.0	36.0	32.0	36.0
35	34.6287421579219	36.0	36.0	36.0	32.0	36.0
36	34.60928771330292	36.0	36.0	36.0	32.0	36.0
37	34.58845062656481	36.0	36.0	36.0	32.0	36.0
38	34.544464462603266	36.0	36.0	36.0	32.0	36.0
39	34.53543032451858	36.0	36.0	36.0	32.0	36.0
40	34.516791103388805	36.0	36.0	36.0	32.0	36.0
41	34.49425913399735	36.0	36.0	36.0	32.0	36.0
42	34.42897778091229	36.0	36.0	36.0	32.0	36.0
43	34.427412731335295	36.0	36.0	36.0	32.0	36.0
44	34.350825321617364	36.0	36.0	36.0	32.0	36.0
45	34.31180807786138	36.0	36.0	36.0	32.0	36.0
46	34.27363811514431	36.0	36.0	36.0	32.0	36.0
47	34.24102500809453	36.0	36.0	36.0	32.0	36.0
48	34.19204459846316	36.0	36.0	36.0	32.0	36.0
49	34.167870000224404	36.0	36.0	36.0	32.0	36.0
50	33.57307486399584	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	4.0
19	18.0
20	29.0
21	110.0
22	400.0
23	1114.0
24	2774.0
25	6246.0
26	12492.0
27	22344.0
28	36746.0
29	54671.0
30	77610.0
31	108132.0
32	155466.0
33	248600.0
34	519109.0
35	1873523.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.72505693942876	17.92354471027577	11.845549234652003	26.505849115643464
2	15.571124938689543	20.644200591786163	37.82549040351096	25.95918406601333
3	18.04314834559967	24.923013498203808	29.03165310520238	28.00218505099414
4	11.992601117526183	16.370380106366948	36.55756413914259	35.07945463696428
5	13.844341361612367	37.37673070696515	33.6652999464639	15.113627984958597
6	33.385533760318935	36.41212199704621	17.116984843496493	13.085359399138358
7	29.64509086712466	30.889693177191695	21.133907590907196	18.331308364776447
8	27.71446587777286	33.54365229857514	19.462208785117856	19.27967303853415
9	27.69201966552199	13.8760800264669	18.38077456715591	40.0511257408552
10	15.411457667131184	27.087310510069212	32.23195391620271	25.269277906596898
11	37.10363606695803	20.78001222676122	23.281593466921546	18.834758239359207
12	25.085473524125092	23.553922613641717	29.408406202885374	21.952197659347814
13	29.736142995128194	19.707448181192476	25.345204927006304	25.211203896673023
14	23.405903216788957	19.98455464283974	25.636920622789628	30.97262151758168
15	24.7422353209343	27.955120845877868	22.538736434513616	24.76390739867422
16	25.17110041877086	25.70457403697609	24.594316731567655	24.530008812685395
17	23.51914957732121	26.14453466863714	25.693356713972925	24.64295904006873
18	24.362614950506288	25.046820111393714	27.1235108447624	23.467054093337598
19	25.456123270373315	24.70039786663213	25.741083103827773	24.102395759166782
20	25.805997632850797	24.5028473752983	25.413738185091578	24.277416806759327
21	26.625301741686673	24.1743097208108	24.79090463199536	24.409483905507166
22	25.64083904946709	24.732904253303616	25.309684242448917	24.316572454780378
23	24.186349345876902	24.482082422491487	25.92372106263867	25.40784716899294
24	24.832458317745264	24.99947906197816	25.752450893176288	24.415611727100288
25	24.793041089794983	24.667565788777686	25.934917621970556	24.604475499456775
26	24.197188661546164	25.59147685996462	26.249396484897215	23.961937993592
27	24.742976991359257	25.106630725550595	25.50054886290482	24.64984342018533
28	24.074003425811437	25.196128185490092	25.93114202370369	24.798726364994785
29	24.281779344508042	24.930705740315315	25.739283032828457	25.048231882348187
30	24.09041990286827	25.300126407821832	26.08264949255739	24.526804196752508
31	24.926310524915284	24.909318825200295	25.189072734092722	24.9752979157917
32	24.512092964655775	25.30554199300931	25.046573053573358	25.135791988761557
33	24.075757760339158	24.92454936398336	25.943926408139372	25.055766467538117
34	24.83157295075214	24.96798162922935	25.921880625255067	24.278564794763447
35	25.1965989739986	24.947311795553738	25.90695202183571	23.949137208611948
36	24.14991383801547	25.505165711537693	25.629399270628785	24.715521179818058
37	25.047454998299283	25.248976443534403	25.128082811656956	24.575485746509358
38	24.437074202907844	25.304714425404356	25.579244345414615	24.67896702627318
39	25.163067836091578	24.789965805926673	25.141844812894366	24.905121545087383
40	25.457641126518006	25.060790668195636	25.622100521557485	23.859467683728873
41	24.35357989545715	25.204284942591194	26.31508081152953	24.12705435042213
42	25.401946062672554	25.722945099643386	25.463658151573863	23.411450686110197
43	24.389295455332334	24.961209842569666	25.95439816269297	24.69509653940503
44	24.147401410423853	25.51143733333846	25.214131423702273	25.127029832535406
45	24.302354209918224	25.685848452945937	25.381906731791386	24.62989060534445
46	24.223205046169785	25.16881689682101	25.381264594358576	25.226713462650626
47	24.723650857410863	25.088568613867128	25.7050465596331	24.482733969088905
48	24.993604363010586	25.51089758838001	24.850015099473794	24.645482949135612
49	24.461350258096655	25.626648167228428	25.12061714946653	24.791384425208392
50	24.409644448248656	26.083807871450098	24.802546941685588	24.704000738615655
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	212.0
1	148.5
2	85.0
3	306.0
4	527.0
5	592.5
6	658.0
7	629.0
8	600.0
9	827.5
10	1055.0
11	1608.0
12	2161.0
13	3916.0
14	5671.0
15	7488.0
16	9305.0
17	10180.0
18	11055.0
19	11109.0
20	11163.0
21	12208.0
22	13253.0
23	15030.0
24	16807.0
25	18974.0
26	21141.0
27	27217.0
28	33293.0
29	40040.5
30	46788.0
31	53061.0
32	59334.0
33	70033.0
34	80732.0
35	93320.5
36	105909.0
37	118554.5
38	131200.0
39	136862.5
40	142525.0
41	151896.5
42	161268.0
43	163962.0
44	166656.0
45	179790.0
46	192924.0
47	205977.5
48	219031.0
49	226220.0
50	233409.0
51	227053.0
52	220697.0
53	218531.0
54	216365.0
55	216197.5
56	216030.0
57	210191.5
58	204353.0
59	185767.5
60	167182.0
61	147411.0
62	127640.0
63	109898.5
64	92157.0
65	78807.5
66	65458.0
67	56147.0
68	46836.0
69	42184.5
70	37533.0
71	29072.5
72	20612.0
73	17492.0
74	14372.0
75	11109.5
76	7847.0
77	6961.0
78	6075.0
79	5040.5
80	4006.0
81	3215.5
82	2425.0
83	1995.5
84	1566.0
85	1113.5
86	661.0
87	483.0
88	305.0
89	242.5
90	180.0
91	141.0
92	102.0
93	84.5
94	67.0
95	77.5
96	88.0
97	61.5
98	35.0
99	35.5
100	36.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009168459217988133
2	6.411509942649043E-4
3	3.8469059655894263E-4
4	0.0
5	0.0
6	9.617264913973566E-5
7	0.0
8	3.205754971324522E-5
9	7.052660936913948E-4
10	9.296689416841114E-4
11	9.617264913973566E-5
12	9.617264913973566E-5
13	3.526330468456974E-4
14	1.9234529827947132E-4
15	0.005417725901538442
16	7.3732364340464E-4
17	0.0
18	1.2823019885298089E-4
19	9.617264913973566E-5
20	8.334962925443756E-4
21	0.0
22	9.617264913973566E-5
23	7.3732364340464E-4
24	5.449783451251687E-4
25	0.001634935035375506
26	0.005257438152972216
27	0.006860315638634477
28	0.004135423913008633
29	0.0026928341759125986
30	0.0038789635153026717
31	0.007052660936913948
32	0.003109582322184786
33	0.004231596562148369
34	0.002789006825052334
35	0.003077524772471541
36	0.008495250674009983
37	0.003814848415876181
38	0.007693811931178852
39	0.004359826761001349
40	0.002532546427346372
41	0.0030454672227582954
42	0.0021157982810741847
43	0.0013464170879562993
44	0.0014105321873827896
45	0.0013464170879562993
46	0.0010899566902503374
47	0.0018272803336549775
48	0.0024363737782066366
49	5.129207954119235E-4
50	0.001314359538243054
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3119390.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.15938518993321
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.66240386342305	32.15573733188436
2	15.50384688716214	14.312960807805283
3	6.171424470898512	8.546074779683524
4	3.02481636781476	5.584946554031048
5	1.6468000006597945	3.800763778061886
6	0.9751595614708843	2.7007659491748544
7	0.6696394521620144	2.1637101787505593
8	0.4524435872195621	1.670761425534633
9	0.3212809010266394	1.334711597819171
>10	1.4127476478817025	11.177762932754625
>50	0.07760567299799749	2.4701730465031364
>100	0.06539351521315084	6.442322165198891
>500	0.010562335670842226	3.295973376668173
>1k	0.005875736399138593	4.343336076129882
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	3171	0.10165449014070058	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.2057549713245215E-5
2	0.0	0.0	0.0	0.0	3.2057549713245215E-5
3	0.0	0.0	0.0	3.2057549713245215E-5	3.2057549713245215E-5
4	0.0	0.0	0.0	3.2057549713245215E-5	3.2057549713245215E-5
5	0.0	0.0	0.0	3.2057549713245215E-5	3.2057549713245215E-5
6	0.0	0.0	0.0	3.2057549713245215E-5	6.411509942649043E-5
7	0.0	0.0	0.0	3.2057549713245215E-5	6.411509942649043E-5
8	0.0	0.0	0.0	3.2057549713245215E-5	6.411509942649043E-5
9	0.0	0.0	0.0	1.9234529827947132E-4	6.411509942649043E-5
10	0.0	0.0	0.0	1.9234529827947132E-4	6.411509942649043E-5
11	0.0	0.0	0.0	1.9234529827947132E-4	6.411509942649043E-5
12	0.0	0.0	0.0	1.9234529827947132E-4	3.526330468456974E-4
13	0.0	0.0	0.0	1.9234529827947132E-4	4.4880569598543306E-4
14	0.0	0.0	0.0	2.2440284799271653E-4	4.4880569598543306E-4
15	0.0	0.0	0.0	2.2440284799271653E-4	5.449783451251687E-4
16	0.0	0.0	0.0	2.564603977059617E-4	5.449783451251687E-4
17	0.0	0.0	0.0	2.8851794741920696E-4	5.770358948384139E-4
18	0.0	0.0	0.0	3.526330468456974E-4	5.770358948384139E-4
19	0.0	0.0	0.0	4.167481462721878E-4	6.411509942649044E-4
20	0.0	0.0	0.0	4.8086324569867825E-4	6.732085439781495E-4
21	0.0	0.0	0.0	6.411509942649044E-4	7.3732364340464E-4
22	0.0	0.0	0.0	8.655538422576209E-4	7.693811931178853E-4
23	0.0	0.0	0.0	0.001634935035375506	7.693811931178853E-4
24	0.0	0.0	0.0	0.002660776626199353	7.693811931178853E-4
25	0.0	0.0	0.0	0.0031095823221847863	7.693811931178853E-4
26	0.0	0.0	0.0	0.0037827908661629355	7.693811931178853E-4
27	0.0	0.0	0.0	0.004872747556413273	7.693811931178853E-4
28	0.0	0.0	0.0	0.007982329878598059	8.014387428311304E-4
29	0.0	0.0	0.0	0.012983307633864313	8.334962925443756E-4
30	0.0	0.0	0.0	0.024972831226618024	8.334962925443756E-4
31	0.0	0.0	0.0	0.053119359874847324	8.655538422576209E-4
32	0.0	0.0	0.0	0.08206732726590776	8.655538422576209E-4
33	0.0	0.0	0.0	0.11181673339979932	8.655538422576209E-4
34	3.2057549713245215E-5	0.0	0.0	0.14435514635874322	9.617264913973565E-4
35	3.2057549713245215E-5	0.0	0.0	0.17881701230048183	9.617264913973565E-4
36	3.2057549713245215E-5	0.0	0.0	0.23158373912848346	9.617264913973565E-4
37	3.2057549713245215E-5	0.0	0.0	0.3127213974527071	9.617264913973565E-4
38	3.2057549713245215E-5	0.0	0.0	0.4223261599222925	9.617264913973565E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	1605	0.0	20.696432	4
ACGATTA	175	0.0	20.114227	40
TTAGGAC	1155	0.0	19.99834	3
ATACCGT	730	0.0	19.587727	6
GTATCAA	6520	0.0	19.435215	1
TACCGTC	730	0.0	18.683678	7
GTCCTAC	1495	0.0	18.541454	1
ACGACTA	60	0.00741753	18.332106	5
TGGCGAG	1910	0.0	18.312614	18
GATATAC	1065	0.0	18.17806	1
TATGGCG	1900	0.0	18.177439	16
ACCGTCG	745	0.0	18.012217	8
AGGACGT	1785	0.0	17.746708	5
CCGTCGT	750	0.0	17.600231	9
GTAGGAC	1815	0.0	17.453096	3
GACGTGA	1010	0.0	17.206766	7
ATTTCGT	820	0.0	17.170132	42
GGACGTG	1795	0.0	16.789959	6
TAATACC	355	0.0	16.731274	4
CGTCGTA	780	0.0	16.641245	10
>>END_MODULE