FastQCFastQC Report
Thu 2 Feb 2017
SRR4064065_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064065_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1682914
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59400.35295921241370626No Hit
TATCAACGCAGAGTACTTTTTTTTT38640.22960175029740085No Hit
GGTATCAACGCAGAGTACTTTTTTT23000.13666770851035762No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20030.11901974788967232No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG402.7630007E-416.6236485
ACGCAAA1500.013.30247719
AATCGTA500.001498876313.30089613
CGCGCCA500.001499212113.300510
CGATAAC1600.013.06299110
GTATCAA32800.012.7684091
GTGTACG604.104401E-412.6622471
GGTATCA11150.012.5202941
CCGATAA1600.012.469969
TATACCG550.003070496712.0899255
AAGACGG2800.011.8740345
ATTCCGA1850.011.8102016
GTATTAG2300.011.5611821
ACGAACG1657.2759576E-1211.51729715
GCCGTGT752.071567E-411.4014458
GATAACG1751.8189894E-1211.40076711
ACACCGT1251.8331775E-811.3994116
CGACCAT2300.011.15259410
CCCGTTC600.005870457311.0847398
AACTTAC600.00587175711.0844087
Produced by FastQC (version 0.11.5)