##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064065_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1682914
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.226523755818775	32.0	32.0	32.0	32.0	32.0
2	30.85347795549862	32.0	32.0	32.0	32.0	32.0
3	30.86625876307405	32.0	32.0	32.0	32.0	32.0
4	30.898306152304873	32.0	32.0	32.0	32.0	32.0
5	30.82383888897472	32.0	32.0	32.0	32.0	32.0
6	34.46443965645303	36.0	36.0	36.0	32.0	36.0
7	34.38962597019218	36.0	36.0	36.0	32.0	36.0
8	34.354842849961436	36.0	36.0	36.0	32.0	36.0
9	34.470294382244134	36.0	36.0	36.0	32.0	36.0
10	34.19411271164183	36.0	36.0	36.0	32.0	36.0
11	34.478338762408534	36.0	36.0	36.0	32.0	36.0
12	34.2845932709574	36.0	36.0	36.0	32.0	36.0
13	34.372058227574314	36.0	36.0	36.0	32.0	36.0
14	34.27358557834803	36.0	36.0	36.0	32.0	36.0
15	34.21306020390822	36.0	36.0	36.0	32.0	36.0
16	34.21104108706684	36.0	36.0	36.0	32.0	36.0
17	34.14461820390109	36.0	36.0	36.0	32.0	36.0
18	34.1339206875693	36.0	36.0	36.0	32.0	36.0
19	34.14252421692374	36.0	36.0	36.0	32.0	36.0
20	34.125701610420975	36.0	36.0	36.0	32.0	36.0
21	34.12869463323735	36.0	36.0	36.0	32.0	36.0
22	34.094435603958374	36.0	36.0	36.0	32.0	36.0
23	34.04510747429756	36.0	36.0	36.0	32.0	36.0
24	34.040172581605475	36.0	36.0	36.0	32.0	36.0
25	33.65085678769088	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	12.0
5	14.0
6	75.0
7	24.0
8	83.0
9	125.0
10	156.0
11	36.0
12	116.0
13	87.0
14	144.0
15	288.0
16	442.0
17	611.0
18	780.0
19	1060.0
20	1582.0
21	2550.0
22	3842.0
23	5971.0
24	9030.0
25	13298.0
26	18849.0
27	25079.0
28	34283.0
29	45578.0
30	59704.0
31	80861.0
32	113783.0
33	160397.0
34	343412.0
35	760639.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.27306166460797	17.351842186918574	11.624305858547912	25.75079028992554
2	16.712967447233453	19.633630589296526	37.64191065444841	26.01149130902161
3	18.813001965673212	23.688976780336613	28.644695653414022	28.853325600576152
4	12.620665189418723	15.42036498601487	36.01805023474932	35.940919589817085
5	14.509877796292288	36.433926563677375	33.66063215043821	15.395563489592124
6	33.987124256922954	35.68576435833147	16.818813109177576	13.508298275568
7	30.364756743649206	30.733364793592564	20.40288943066044	18.498989032097786
8	28.22711274617325	32.557847852435444	19.229674118959686	19.985365282431623
9	27.399398014028904	14.261170121378111	18.67072033215643	39.668711532436554
10	15.936833180832588	26.59601096102818	31.336614031614324	26.130541826524905
11	36.76959682089345	21.473000305591857	22.380203830956575	19.377199042558125
12	24.831079474104797	23.931240248867915	28.619394288214924	22.61828598881236
13	29.08942262812566	19.78820878261671	25.481717994226578	25.64065059503105
14	23.676067660517727	19.565907642251673	25.448386498339882	31.309638198890717
15	25.00255661413915	27.23287544175914	22.563071076251315	25.2014968678504
16	25.553190350757227	26.11628086740913	23.667520915215338	24.663007866618305
17	23.745632075858737	25.929103619022477	25.335178905772942	24.990085399345844
18	24.360042458678606	25.242398023352276	26.33609552608027	24.061463991888846
19	25.311834734726403	24.82475649061047	26.051877311703915	23.811531462959216
20	25.857697092524006	24.106087312291418	25.371016581866275	24.6651990133183
21	26.76787828441729	24.08878920086984	25.00334484753739	24.13998766717548
22	25.96131717832454	24.063688396640153	25.401085259793355	24.573909165241954
23	24.477414182934474	24.241499766285944	25.79257609979412	25.488509950985467
24	24.45694672312712	24.897407247291202	25.79087550374662	24.854770525835058
25	24.66729662114066	24.48407858454106	25.774930230262434	25.07369456405584
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	107.0
1	107.0
2	325.0
3	543.0
4	543.0
5	543.0
6	1489.0
7	2435.0
8	2435.0
9	2435.0
10	2717.5
11	3000.0
12	3000.0
13	3000.0
14	3578.0
15	4156.0
16	4156.0
17	4156.0
18	7699.5
19	11243.0
20	11243.0
21	11243.0
22	17808.5
23	24374.0
24	24374.0
25	24374.0
26	36229.0
27	48084.0
28	48084.0
29	48084.0
30	60235.5
31	72387.0
32	72387.0
33	72387.0
34	89216.5
35	106046.0
36	106046.0
37	106046.0
38	123420.0
39	140794.0
40	140794.0
41	140794.0
42	160890.0
43	180986.0
44	180986.0
45	180986.0
46	200843.0
47	220700.0
48	220700.0
49	220700.0
50	226428.0
51	232156.0
52	232156.0
53	232156.0
54	214881.0
55	197606.0
56	197606.0
57	197606.0
58	179878.0
59	162150.0
60	162150.0
61	162150.0
62	140804.5
63	119459.0
64	119459.0
65	119459.0
66	97875.0
67	76291.0
68	76291.0
69	76291.0
70	57651.0
71	39011.0
72	39011.0
73	39011.0
74	29783.0
75	20555.0
76	20555.0
77	20555.0
78	16229.0
79	11903.0
80	11903.0
81	11903.0
82	8220.5
83	4538.0
84	4538.0
85	4538.0
86	3415.5
87	2293.0
88	2293.0
89	2293.0
90	1608.0
91	923.0
92	923.0
93	923.0
94	647.5
95	372.0
96	372.0
97	372.0
98	587.0
99	802.0
100	802.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03684086055496597
2	0.004575397197955451
3	0.0017826222849177081
4	0.003268140855682465
5	0.008913111424588541
6	0.012775459708576908
7	0.02186683336165722
8	0.03642491535515184
9	0.04842790540693107
10	0.056746809403213715
11	0.05538013231811014
12	0.0628671459147645
13	0.06352077408590101
14	0.06571934157063285
15	0.05953958431625145
16	0.06678891494158347
17	0.062451200714950375
18	0.07599913008032497
19	0.07451361150956021
20	0.07831653905071798
21	0.0726715684818119
22	0.07659333750863086
23	0.08152525916356985
24	0.07516723968069669
25	0.07790059385090385
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1682914.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.54488406995254
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.07081336172021	28.5351997895181
2	16.192597641149977	13.778243788682254
3	6.558528984956996	8.370955660032566
4	3.391417566616909	5.771498688180678
5	1.8434910237871294	3.9215555945510765
6	1.149300555999082	2.933811534990776
7	0.7589173695128573	2.260163605321849
8	0.5383670062256665	1.8323809493566716
9	0.3792491649031335	1.4521600578987115
>10	1.8862269512130276	14.264035955725296
>50	0.12416288644422081	3.6691721185831603
>100	0.09445518287977256	8.262522782073866
>500	0.00910898649915969	2.6514347365082824
>1k	0.0032231798381641983	1.9427120922485637
>5k	1.4013825383322602E-4	0.35415264632815086
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5940	0.35295921241370626	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3864	0.22960175029740085	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2300	0.13666770851035762	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2003	0.11901974788967232	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.1884148566118055E-4
7	0.0	0.0	0.0	0.0	1.1884148566118055E-4
8	0.0	0.0	0.0	5.9420742830590276E-5	1.1884148566118055E-4
9	0.0	0.0	0.0	1.1884148566118055E-4	1.1884148566118055E-4
10	0.0	0.0	0.0	1.1884148566118055E-4	1.7826222849177083E-4
11	0.0	0.0	0.0	1.1884148566118055E-4	1.7826222849177083E-4
12	0.0	0.0	0.0	1.1884148566118055E-4	7.724696567976736E-4
13	0.0	0.0	0.0	1.1884148566118055E-4	8.913111424588541E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGATCG	40	2.7630007E-4	16.623648	5
ACGCAAA	150	0.0	13.302477	19
AATCGTA	50	0.0014988763	13.300896	13
CGCGCCA	50	0.0014992121	13.3005	10
CGATAAC	160	0.0	13.062991	10
GTATCAA	3280	0.0	12.768409	1
GTGTACG	60	4.104401E-4	12.662247	1
GGTATCA	1115	0.0	12.520294	1
CCGATAA	160	0.0	12.46996	9
TATACCG	55	0.0030704967	12.089925	5
AAGACGG	280	0.0	11.874034	5
ATTCCGA	185	0.0	11.810201	6
GTATTAG	230	0.0	11.561182	1
ACGAACG	165	7.2759576E-12	11.517297	15
GCCGTGT	75	2.071567E-4	11.401445	8
GATAACG	175	1.8189894E-12	11.400767	11
ACACCGT	125	1.8331775E-8	11.399411	6
CGACCAT	230	0.0	11.152594	10
CCCGTTC	60	0.0058704573	11.084739	8
AACTTAC	60	0.005871757	11.084408	7
>>END_MODULE