FastQCFastQC Report
Thu 2 Feb 2017
SRR4064065_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064065_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1682914
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAT1107.2759576E-1225.9999648
GTATCAA33550.024.5915931
TATGCGA1153.5106495E-1022.9564912
GGTATCA15150.021.7834431
CCGTATG1259.968062E-1021.12069
CGACGGT3750.019.3599747
CGCAATA3450.019.13154636
TACGACG3900.018.6153585
TACCGTA1456.3118932E-918.2068717
ACGACGG4000.018.1499756
ATACCGT4250.018.1176226
CGTCGTA4300.017.90695410
CGTCTTA1751.9099389E-1017.59997715
ACGCTTA1509.607902E-917.59945317
CATCGTT3400.017.47056428
CTTATAT1903.274181E-1117.3683993
CGCTTAC1408.380812E-817.28517718
CTAGCGG3700.017.2432229
ATCGTTT3450.017.21736729
AACGCAG47350.017.1911096
Produced by FastQC (version 0.11.5)