##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064065_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1682914
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.339403558351762	32.0	32.0	32.0	32.0	32.0
2	31.44412608130897	32.0	32.0	32.0	32.0	32.0
3	31.502803470646747	32.0	32.0	32.0	32.0	32.0
4	31.577599330684752	32.0	32.0	32.0	32.0	32.0
5	31.53319955743431	32.0	32.0	32.0	32.0	32.0
6	35.117090356369964	36.0	36.0	36.0	36.0	36.0
7	35.13396287629671	36.0	36.0	36.0	36.0	36.0
8	35.08902475111622	36.0	36.0	36.0	36.0	36.0
9	35.181192859528174	36.0	36.0	36.0	36.0	36.0
10	35.05073758968076	36.0	36.0	36.0	36.0	36.0
11	35.187748155877244	36.0	36.0	36.0	36.0	36.0
12	35.095647787112114	36.0	36.0	36.0	36.0	36.0
13	35.141900893331446	36.0	36.0	36.0	36.0	36.0
14	35.07991555124029	36.0	36.0	36.0	36.0	36.0
15	35.06205189332313	36.0	36.0	36.0	36.0	36.0
16	35.06951810965979	36.0	36.0	36.0	36.0	36.0
17	35.03485858457414	36.0	36.0	36.0	36.0	36.0
18	35.02990289462207	36.0	36.0	36.0	36.0	36.0
19	35.02677498671947	36.0	36.0	36.0	36.0	36.0
20	35.01762894598298	36.0	36.0	36.0	36.0	36.0
21	35.02052570719597	36.0	36.0	36.0	36.0	36.0
22	35.005167822003976	36.0	36.0	36.0	36.0	36.0
23	34.95021967848624	36.0	36.0	36.0	36.0	36.0
24	34.93323901280755	36.0	36.0	36.0	36.0	36.0
25	34.90494166665676	36.0	36.0	36.0	32.0	36.0
26	34.85199837900213	36.0	36.0	36.0	32.0	36.0
27	34.852329352539705	36.0	36.0	36.0	32.0	36.0
28	34.825916238144075	36.0	36.0	36.0	32.0	36.0
29	34.8067738458412	36.0	36.0	36.0	32.0	36.0
30	34.788867404989205	36.0	36.0	36.0	32.0	36.0
31	34.780181280802225	36.0	36.0	36.0	32.0	36.0
32	34.749659816247295	36.0	36.0	36.0	32.0	36.0
33	34.722883046905544	36.0	36.0	36.0	32.0	36.0
34	34.71513636466272	36.0	36.0	36.0	32.0	36.0
35	34.66965632230762	36.0	36.0	36.0	32.0	36.0
36	34.65028516014485	36.0	36.0	36.0	32.0	36.0
37	34.641932386325145	36.0	36.0	36.0	32.0	36.0
38	34.60174376111911	36.0	36.0	36.0	32.0	36.0
39	34.59959391864349	36.0	36.0	36.0	32.0	36.0
40	34.57843656895123	36.0	36.0	36.0	32.0	36.0
41	34.549278216236836	36.0	36.0	36.0	32.0	36.0
42	34.48569683299325	36.0	36.0	36.0	32.0	36.0
43	34.46691512459935	36.0	36.0	36.0	32.0	36.0
44	34.41981705541697	36.0	36.0	36.0	32.0	36.0
45	34.37689626445558	36.0	36.0	36.0	32.0	36.0
46	34.34322431211577	36.0	36.0	36.0	32.0	36.0
47	34.30727951636269	36.0	36.0	36.0	32.0	36.0
48	34.25654252088936	36.0	36.0	36.0	32.0	36.0
49	34.23539289589367	36.0	36.0	36.0	32.0	36.0
50	33.651895462275554	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	1.0
18	2.0
19	4.0
20	21.0
21	52.0
22	132.0
23	488.0
24	1241.0
25	2813.0
26	5977.0
27	10974.0
28	18743.0
29	28547.0
30	41302.0
31	58833.0
32	84545.0
33	134922.0
34	282176.0
35	1012139.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.87857156864668	18.044128457322426	12.021673800741516	26.055626173289383
2	16.071248186173204	20.422946973865443	37.0038492940188	26.50195554594255
3	18.133373898038396	24.095315964984422	28.986195323808552	28.785114813168633
4	12.303837272730513	16.0018872027923	36.00605853893901	35.688216985538176
5	14.082597209364234	36.93236849892508	33.397012562733444	15.588021728977239
6	33.99811516731752	35.967444447349465	16.65807043522284	13.376369950110167
7	30.32894134816158	30.929150271493373	20.371332106096926	18.370576274248123
8	28.387309155429215	32.77701653203907	19.00400139282221	19.831672919709504
9	27.59857887409226	14.083146204953263	18.490918306652066	39.82735661430241
10	15.94295561233585	26.83403648463961	31.353734624754885	25.869273278269656
11	36.98551262008197	21.4191702126016	22.308699261340305	19.286617905976126
12	24.809452193253236	24.0102417774915	28.56015558755038	22.62015044170488
13	29.191707677077627	19.782031795002215	25.447291767929997	25.57896875999016
14	23.68866710321313	19.587962726379253	25.48187480465338	31.241495365754236
15	25.15914994054718	27.285130496117592	22.391994043427594	25.163725519907633
16	25.603573368257297	26.173425064204874	23.626621759896942	24.596379807640886
17	23.90751993268818	26.055817468985342	25.18821520291589	24.84844739541058
18	24.548846937744738	25.21501446898527	26.229923168796905	24.006215424473083
19	25.409438628474184	24.914523261438195	25.916416406304776	23.759621703782845
20	25.97149092966142	24.12515754027416	25.221715095878967	24.681636434185453
21	26.909396439746775	24.13480427401519	24.94221332759725	24.01358595864079
22	26.09130715610373	24.14735639929099	25.29441510048351	24.46692134412177
23	24.547552113879544	24.252288161929904	25.714227990832494	25.485931733358054
24	24.59904201479939	24.919558834801922	25.649486275831045	24.83191287456764
25	24.835134797877462	24.443962469323605	25.783087426985723	24.937815305813213
26	24.630815572092505	25.208829644806872	25.915436547104253	24.244918235996366
27	25.105834757536787	24.863324007672873	25.359695557198307	24.67114567759203
28	24.704963739954504	24.551235884015394	25.9180292409754	24.825771135054705
29	24.403760220574252	24.841521677124927	25.978085187297967	24.776632915002853
30	24.623142452390688	24.876401182745607	25.944283079321917	24.556173285541792
31	24.943887529036754	24.927723809574744	25.20743935188239	24.92094930950611
32	24.85031505739012	24.94158781318559	25.090143730821428	25.11795339860286
33	24.567606465728808	24.614491437698845	25.479991585662827	25.337910510909524
34	25.06472585177557	24.79696850672097	25.626980622948814	24.511325018554643
35	25.35099536086548	24.693363726091995	25.674849378170578	24.28079153487195
36	24.598033383864525	25.057686970856118	25.38072212261906	24.9635575226603
37	25.52235417996247	24.896990959448846	25.081082108931096	24.499572751657592
38	24.82325388746367	24.75889613681162	25.452331586819675	24.965518388905032
39	25.255755083607667	24.940517927813367	24.930950881237894	24.872776107341075
40	25.22589385989411	25.02908721410448	25.369992928746726	24.37502599725469
41	24.429230934699895	25.354262770365466	25.774796611247158	24.44170968368748
42	25.498665392669285	25.17624422719937	25.25087765584482	24.074212724286525
43	25.010933559610482	24.52670759620938	25.384397810435843	25.077961033744295
44	24.67548441879549	24.977404980589974	25.17807133311506	25.16903926749948
45	24.87843835399916	25.33063638702685	25.06020889053421	24.730716368439786
46	24.82854022397026	25.06224367194287	24.96681327849156	25.142402825595312
47	24.924831241680927	24.89262454839323	25.366514546491732	24.816029663434115
48	25.560242606901117	25.403309907319983	24.43158690987571	24.604860575903196
49	24.96516448601796	25.36429480144203	24.743405614920356	24.927135097619658
50	24.868857017902474	25.57383225107196	24.763443492646008	24.793867238379555
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	21.5
2	33.0
3	87.5
4	142.0
5	184.0
6	226.0
7	222.0
8	218.0
9	309.5
10	401.0
11	629.5
12	858.0
13	1704.5
14	2551.0
15	3500.0
16	4449.0
17	4785.0
18	5121.0
19	5182.0
20	5243.0
21	5751.5
22	6260.0
23	7156.5
24	8053.0
25	9127.5
26	10202.0
27	12470.5
28	14739.0
29	17432.0
30	20125.0
31	22760.5
32	25396.0
33	29681.0
34	33966.0
35	39746.5
36	45527.0
37	53547.0
38	61567.0
39	68037.5
40	74508.0
41	82225.5
42	89943.0
43	92917.0
44	95891.0
45	105244.0
46	114597.0
47	121089.5
48	127582.0
49	130788.0
50	133994.0
51	131158.5
52	128323.0
53	125950.5
54	123578.0
55	121554.5
56	119531.0
57	115014.0
58	110497.0
59	100403.5
60	90310.0
61	79546.0
62	68782.0
63	59928.5
64	51075.0
65	42942.0
66	34809.0
67	30276.5
68	25744.0
69	22863.0
70	19982.0
71	15279.5
72	10577.0
73	8914.0
74	7251.0
75	5614.5
76	3978.0
77	3336.5
78	2695.0
79	2270.5
80	1846.0
81	1439.5
82	1033.0
83	851.5
84	670.0
85	474.5
86	279.0
87	202.0
88	125.0
89	92.0
90	59.0
91	57.0
92	55.0
93	44.5
94	34.0
95	35.5
96	37.0
97	34.5
98	32.0
99	21.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00861600771043559
2	4.753659426447222E-4
3	3.565244569835416E-4
4	0.0
5	0.0
6	3.565244569835416E-4
7	0.0
8	0.0
9	0.0013666770851035763
10	8.318903996282638E-4
11	5.9420742830590276E-5
12	1.782622284917708E-4
13	4.753659426447222E-4
14	4.753659426447222E-4
15	0.0039811897696495485
16	0.0011884148566118054
17	0.0
18	2.376829713223611E-4
19	0.0
20	6.536281711364931E-4
21	0.0
22	5.9420742830590276E-5
23	7.724696567976737E-4
24	4.159451998141319E-4
25	0.0014260978279341664
26	0.0049319216549389925
27	0.006060915768720207
28	0.004159451998141319
29	0.0020203052562400695
30	0.002971037141529514
31	0.007902958796468507
32	0.0027333541702071525
33	0.0042782934838025
34	0.002792774913037743
35	0.003386982341343646
36	0.007427592853823784
37	0.0030898786271906947
38	0.007902958796468507
39	0.0032087201128518745
40	0.0026145126845459723
41	0.0031492993700212844
42	0.0013072563422729861
43	0.0013072563422729861
44	0.0012478355994423958
45	0.0013666770851035763
46	7.130489139670832E-4
47	0.0020203052562400695
48	0.0016043600564259373
49	2.9710371415295135E-4
50	0.001069573370950625
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1682914.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.19677865317719
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.05352664242405	30.75800180225737
2	15.775527310705753	14.260060319982394
3	6.543933482103813	8.872941393352836
4	3.2369816709771446	5.852045763501826
5	1.782601164521612	4.028391512988961
6	1.1222316120754794	3.0432752261144196
7	0.7352545087255364	2.3261794699233027
8	0.48894094632542207	1.7678844580436073
9	0.36367742936713704	1.4793343448636678
>10	1.6983868926724408	13.6130099525773
>50	0.11302530264859392	3.495842148084306
>100	0.07718978864658683	7.227725718921746
>500	0.007005033132497192	2.2889341844222697
>1k	0.001718215674008745	0.9863737049659556
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.376829713223611E-4
2	0.0	0.0	0.0	0.0	2.376829713223611E-4
3	0.0	0.0	0.0	0.0	2.376829713223611E-4
4	0.0	0.0	0.0	0.0	2.376829713223611E-4
5	0.0	0.0	0.0	0.0	2.9710371415295135E-4
6	0.0	0.0	0.0	0.0	3.5652445698354166E-4
7	0.0	0.0	0.0	0.0	3.5652445698354166E-4
8	0.0	0.0	0.0	0.0	3.5652445698354166E-4
9	0.0	0.0	0.0	0.0	3.5652445698354166E-4
10	0.0	0.0	0.0	0.0	4.159451998141319E-4
11	0.0	0.0	0.0	0.0	4.159451998141319E-4
12	0.0	0.0	0.0	0.0	0.0010101526281200346
13	0.0	0.0	0.0	0.0	0.001069573370950625
14	0.0	0.0	0.0	0.0	0.001069573370950625
15	0.0	0.0	0.0	0.0	0.0013666770851035763
16	0.0	0.0	0.0	0.0	0.0013666770851035763
17	0.0	0.0	0.0	0.0	0.0014260978279341666
18	0.0	0.0	0.0	0.0	0.0014855185707647568
19	0.0	0.0	0.0	0.0	0.0014855185707647568
20	0.0	0.0	0.0	0.0	0.001723201542087118
21	0.0	0.0	0.0	0.0	0.0018420430277482985
22	0.0	0.0	0.0	0.0	0.0019014637705788888
23	0.0	0.0	0.0	5.9420742830590276E-5	0.0019014637705788888
24	0.0	0.0	0.0	1.7826222849177083E-4	0.001960884513409479
25	0.0	0.0	0.0	1.7826222849177083E-4	0.001960884513409479
26	0.0	0.0	0.0	1.7826222849177083E-4	0.0022579882275624305
27	0.0	0.0	0.0	1.7826222849177083E-4	0.0023174089703930207
28	0.0	0.0	0.0	5.942074283059027E-4	0.0023174089703930207
29	0.0	0.0	0.0	0.0016043600564259375	0.0023174089703930207
30	0.0	0.0	0.0	0.004218872740971909	0.0023174089703930207
31	0.0	0.0	0.0	0.010458050738183889	0.0024362504560542014
32	0.0	0.0	0.0	0.01699433244954882	0.0024956711988847915
33	0.0	0.0	0.0	0.025075553474509096	0.0026145126845459723
34	0.0	0.0	0.0	0.033810402670605866	0.0026739334273765624
35	0.0	0.0	0.0	0.04611049643653805	0.0026739334273765624
36	0.0	0.0	0.0	0.06441208522835985	0.0026739334273765624
37	0.0	0.0	0.0	0.09358766995817969	0.0027333541702071525
38	0.0	0.0	0.0	0.13963874565188714	0.0027333541702071525
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTAT	110	7.2759576E-12	25.999964	8
GTATCAA	3355	0.0	24.591593	1
TATGCGA	115	3.5106495E-10	22.95649	12
GGTATCA	1515	0.0	21.783443	1
CCGTATG	125	9.968062E-10	21.1206	9
CGACGGT	375	0.0	19.359974	7
CGCAATA	345	0.0	19.131546	36
TACGACG	390	0.0	18.615358	5
TACCGTA	145	6.3118932E-9	18.206871	7
ACGACGG	400	0.0	18.149975	6
ATACCGT	425	0.0	18.117622	6
CGTCGTA	430	0.0	17.906954	10
CGTCTTA	175	1.9099389E-10	17.599977	15
ACGCTTA	150	9.607902E-9	17.599453	17
CATCGTT	340	0.0	17.470564	28
CTTATAT	190	3.274181E-11	17.368399	3
CGCTTAC	140	8.380812E-8	17.285177	18
CTAGCGG	370	0.0	17.24322	29
ATCGTTT	345	0.0	17.217367	29
AACGCAG	4735	0.0	17.191109	6
>>END_MODULE