FastQCFastQC Report
Thu 2 Feb 2017
SRR4064064_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064064_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2762986
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48290.1747746821735615No Hit
GGGTAGGCACACGCTGAGCCAGTCA31260.11313846686157657No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG31160.11277653958434823No Hit
TATCAACGCAGAGTACTTTTTTTTT30900.11183552866355458No Hit
GCGCAAGACGGACCAGAGCGAAAGC28930.10470556130215644No Hit
GAATAGGACCGCGGTTCTATTTTGT28440.10293211764373761No Hit
GATTAAGAGGGACGGCCGGGGGCAT28160.10191872126749828No Hit
GTCTTGCGCCGGTCCAAGAATTTCA27930.10108628852987311No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA2350.014.96099816
GTATCAA32200.013.6862451
GGTATCA9850.013.4993861
GCGTTAT3700.013.09153651
ACGAACG2850.012.66944415
CGTTATT3600.012.6609592
CGGTCCA6650.012.5716810
AGGCCCG6300.012.36532710
GTTATTC3950.012.2616273
TAGATCG550.0030744412.08830455
CGCATCG4050.011.96385213
GCGTAAC2600.011.32714911
CGTCAAT5550.011.30059319
TAACGAA3150.011.1595613
ATTTGCG2500.011.02261516
AAGACGG6300.011.0055665
TTAGTAC951.3663159E-510.9962373
AACGAAC3200.010.98558914
TAATTGC5900.010.94996713
CAAGACG6250.010.9410494
Produced by FastQC (version 0.11.5)