##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064064_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2762986
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.236885384145992	32.0	32.0	32.0	32.0	32.0
2	30.75756446105771	32.0	32.0	32.0	32.0	32.0
3	30.78068654709072	32.0	32.0	32.0	32.0	32.0
4	30.82451774999946	32.0	32.0	32.0	32.0	32.0
5	30.6936383318627	32.0	32.0	32.0	32.0	32.0
6	34.35951647963471	36.0	36.0	36.0	32.0	36.0
7	34.30047311133679	36.0	36.0	36.0	32.0	36.0
8	34.25096218366651	36.0	36.0	36.0	32.0	36.0
9	34.42594280246082	36.0	36.0	36.0	32.0	36.0
10	34.057643071662326	36.0	36.0	36.0	32.0	36.0
11	34.39509103556804	36.0	36.0	36.0	32.0	36.0
12	34.174844896065345	36.0	36.0	36.0	32.0	36.0
13	34.28038433781423	36.0	36.0	36.0	32.0	36.0
14	34.15530987127694	36.0	36.0	36.0	32.0	36.0
15	34.07939417716919	36.0	36.0	36.0	32.0	36.0
16	34.08344595303777	36.0	36.0	36.0	32.0	36.0
17	33.99799094168411	36.0	36.0	36.0	32.0	36.0
18	33.998439369580595	36.0	36.0	36.0	32.0	36.0
19	34.01851402793934	36.0	36.0	36.0	32.0	36.0
20	34.00973982495749	36.0	36.0	36.0	32.0	36.0
21	33.99375711639509	36.0	36.0	36.0	32.0	36.0
22	33.96606569848707	36.0	36.0	36.0	32.0	36.0
23	33.89642690914829	36.0	36.0	36.0	32.0	36.0
24	33.8832683191301	36.0	36.0	36.0	32.0	36.0
25	33.46666903125821	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	11.0
4	26.0
5	26.0
6	114.0
7	50.0
8	156.0
9	175.0
10	250.0
11	92.0
12	159.0
13	127.0
14	244.0
15	404.0
16	734.0
17	964.0
18	1273.0
19	1768.0
20	2858.0
21	4408.0
22	7034.0
23	11044.0
24	16483.0
25	23746.0
26	33870.0
27	44284.0
28	59893.0
29	80229.0
30	105284.0
31	141885.0
32	198901.0
33	278340.0
34	578411.0
35	1169741.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.39075627665926	17.204760858223153	11.387579522936605	26.01690334218098
2	16.985131241765718	19.42251451405076	37.089914725427455	26.50243951875606
3	18.880558768728292	23.18573167924509	28.64909451264092	29.2846150393857
4	12.60307142159116	15.352656095610032	35.91518301488659	36.12908946791222
5	14.832343127765837	36.230447753414566	33.635293547025654	15.301915571793945
6	34.52992257377933	35.68259231239797	16.577681412277432	13.209803701545267
7	30.589666714210313	30.437442576566387	20.46482482096921	18.508065888254087
8	28.355378420856546	32.79746385849918	19.084710992903307	19.76244672774097
9	27.21247982630617	14.097089375483634	18.42553032780148	40.26490047040872
10	15.839138883436817	26.397333563165276	31.462846531408633	26.30068102198927
11	37.31016074345614	21.033785856212663	22.499086540881503	19.156966859449696
12	24.73993944837177	23.45897554757214	28.676048788967435	23.125036215088652
13	29.286669088293653	19.34102531879826	25.28479166342234	26.087513929485745
14	23.6901672271811	19.716719893204075	24.76464550691486	31.828467372699965
15	25.25982884378111	27.36605492194542	22.009555516041683	25.364560718231782
16	26.07406345056974	25.82264856276662	23.44217475688774	24.661113229775896
17	24.125204658815058	25.97288309178674	24.98588528676498	24.916026962633218
18	24.954706306345464	24.93985591498332	25.878255767203818	24.2271820114674
19	25.517832384136952	24.93197949098348	25.20112773512712	24.34906038975245
20	25.881419717983363	24.321381613500286	24.626652129975408	25.170546538540943
21	26.98218148259221	24.18868446634996	24.27955905000746	24.549575001050364
22	26.21134340813675	24.111542656130418	24.712800008403114	24.96431392732972
23	24.605630241812506	24.15096854677053	25.279979889406224	25.96342132201074
24	24.875618106294723	24.88612211013122	25.072224081551635	25.16603570202242
25	24.956806905679322	24.459316632727944	25.09955238552653	25.484324076066205
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	102.0
1	102.0
2	317.0
3	532.0
4	532.0
5	532.0
6	1566.5
7	2601.0
8	2601.0
9	2601.0
10	3072.5
11	3544.0
12	3544.0
13	3544.0
14	5061.0
15	6578.0
16	6578.0
17	6578.0
18	11286.5
19	15995.0
20	15995.0
21	15995.0
22	26472.0
23	36949.0
24	36949.0
25	36949.0
26	55535.5
27	74122.0
28	74122.0
29	74122.0
30	93310.5
31	112499.0
32	112499.0
33	112499.0
34	141335.0
35	170171.0
36	170171.0
37	170171.0
38	197727.0
39	225283.0
40	225283.0
41	225283.0
42	260164.5
43	295046.0
44	295046.0
45	295046.0
46	327189.0
47	359332.0
48	359332.0
49	359332.0
50	369984.5
51	380637.0
52	380637.0
53	380637.0
54	353268.0
55	325899.0
56	325899.0
57	325899.0
58	299750.0
59	273601.0
60	273601.0
61	273601.0
62	239724.5
63	205848.0
64	205848.0
65	205848.0
66	169784.5
67	133721.0
68	133721.0
69	133721.0
70	100330.0
71	66939.0
72	66939.0
73	66939.0
74	51399.0
75	35859.0
76	35859.0
77	35859.0
78	28581.0
79	21303.0
80	21303.0
81	21303.0
82	14620.0
83	7937.0
84	7937.0
85	7937.0
86	6100.0
87	4263.0
88	4263.0
89	4263.0
90	3042.5
91	1822.0
92	1822.0
93	1822.0
94	1300.0
95	778.0
96	778.0
97	778.0
98	1201.5
99	1625.0
100	1625.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.037893785925806354
2	0.005284138247533647
3	0.0019544072970329924
4	0.003474501861391987
5	0.009410109207936631
6	0.012884611069328616
7	0.022149949366373916
8	0.03601176408421903
9	0.04867921878721065
10	0.05602634251494578
11	0.05447005522286396
12	0.06174479349515343
13	0.06359062260901792
14	0.06630507718823042
15	0.059681808014951945
16	0.06561741536149658
17	0.06091236075752827
18	0.07658381186151504
19	0.07433986274269938
20	0.07803152097042837
21	0.07376077909913405
22	0.07618569185656388
23	0.08150602283182036
24	0.07723528096052604
25	0.07701812459418904
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2762986.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.16265077828186
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.22650087445747	34.72654689139695
2	13.873525475900541	12.808774232151865
3	4.5402814757823196	6.287742846049241
4	1.9829934383273284	3.6616093435651544
5	1.0860240889272263	2.5066875376974638
6	0.6272461553333492	1.7373207132404003
7	0.4129781172753346	1.3344915224797522
8	0.3151849935813866	1.1639819831402045
9	0.23369043172540055	0.9708992810969026
>10	1.4790900925662769	12.768779155420606
>50	0.11578631200942892	3.682127223997251
>100	0.08652854494061595	8.428925460026058
>500	0.012685038542587915	4.111973148561873
>1k	0.007484960630716239	5.81014066117629
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4829	0.1747746821735615	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	3126	0.11313846686157657	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	3116	0.11277653958434823	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3090	0.11183552866355458	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2893	0.10470556130215644	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2844	0.10293211764373761	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2816	0.10191872126749828	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2793	0.10108628852987311	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4477091089133278E-4
2	0.0	0.0	0.0	0.0	1.4477091089133278E-4
3	0.0	0.0	0.0	0.0	1.4477091089133278E-4
4	0.0	0.0	0.0	0.0	1.4477091089133278E-4
5	0.0	3.6192727722833194E-5	0.0	0.0	1.4477091089133278E-4
6	0.0	3.6192727722833194E-5	0.0	0.0	1.4477091089133278E-4
7	0.0	3.6192727722833194E-5	0.0	3.6192727722833194E-5	1.4477091089133278E-4
8	0.0	3.6192727722833194E-5	0.0	3.6192727722833194E-5	1.4477091089133278E-4
9	0.0	3.6192727722833194E-5	0.0	7.238545544566639E-5	1.4477091089133278E-4
10	0.0	3.6192727722833194E-5	0.0	7.238545544566639E-5	1.8096363861416597E-4
11	0.0	3.6192727722833194E-5	0.0	7.238545544566639E-5	1.8096363861416597E-4
12	0.0	3.6192727722833194E-5	0.0	7.238545544566639E-5	2.533490940598324E-4
13	0.0	3.6192727722833194E-5	0.0	7.238545544566639E-5	3.2573454950549877E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	235	0.0	14.960998	16
GTATCAA	3220	0.0	13.686245	1
GGTATCA	985	0.0	13.499386	1
GCGTTAT	370	0.0	13.0915365	1
ACGAACG	285	0.0	12.669444	15
CGTTATT	360	0.0	12.660959	2
CGGTCCA	665	0.0	12.57168	10
AGGCCCG	630	0.0	12.365327	10
GTTATTC	395	0.0	12.261627	3
TAGATCG	55	0.00307444	12.0883045	5
CGCATCG	405	0.0	11.963852	13
GCGTAAC	260	0.0	11.327149	11
CGTCAAT	555	0.0	11.300593	19
TAACGAA	315	0.0	11.15956	13
ATTTGCG	250	0.0	11.022615	16
AAGACGG	630	0.0	11.005566	5
TTAGTAC	95	1.3663159E-5	10.996237	3
AACGAAC	320	0.0	10.985589	14
TAATTGC	590	0.0	10.949967	13
CAAGACG	625	0.0	10.941049	4
>>END_MODULE