FastQCFastQC Report
Thu 2 Feb 2017
SRR4064064_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064064_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2762986
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT30150.10912107408434209No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC29930.10832483407443975No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA29900.10821625589127126No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT29310.1060808849556241No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA28870.10448840493581944No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG27940.10112248125759594No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC27880.10090532489125895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37850.027.9003941
GGTATCA15900.023.3843121
TCAACGC54450.019.0691784
AACGCAG56400.018.4488756
CAACGCA56350.018.4262075
TCGATCG1801.4551915E-1118.33285741
ATCAACG57050.018.2001193
TATCAAC59300.017.694722
TAGGACG4000.017.0486324
CGAGCCG9700.017.00894415
CTAGCGG9100.016.92447729
CGCAATA9000.016.86897736
ACCCGTT4050.016.84150930
ACGACGG8550.016.4665156
CGGTCCA10050.016.41659410
CGACGGT8450.016.1407177
TAGCGGC9550.016.12757130
GTCCTAC7650.016.1050341
ATACGAA9500.015.97853440
GATATAC4700.015.9153461
Produced by FastQC (version 0.11.5)