##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064064_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2762986
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.350023489080293	32.0	32.0	32.0	32.0	32.0
2	31.413729566490748	32.0	32.0	32.0	32.0	32.0
3	31.485952878516215	32.0	32.0	32.0	32.0	32.0
4	31.56740099298368	32.0	32.0	32.0	32.0	32.0
5	31.518727565032904	32.0	32.0	32.0	32.0	32.0
6	35.106073284482804	36.0	36.0	36.0	36.0	36.0
7	35.11621593449985	36.0	36.0	36.0	36.0	36.0
8	35.07100578866487	36.0	36.0	36.0	36.0	36.0
9	35.173261102300195	36.0	36.0	36.0	36.0	36.0
10	35.03695277500501	36.0	36.0	36.0	36.0	36.0
11	35.179948070674264	36.0	36.0	36.0	36.0	36.0
12	35.07609086690993	36.0	36.0	36.0	36.0	36.0
13	35.12346678557184	36.0	36.0	36.0	36.0	36.0
14	35.067576889640414	36.0	36.0	36.0	36.0	36.0
15	35.03907366884957	36.0	36.0	36.0	36.0	36.0
16	35.05136616689335	36.0	36.0	36.0	36.0	36.0
17	35.00931745582496	36.0	36.0	36.0	36.0	36.0
18	35.010223359799866	36.0	36.0	36.0	36.0	36.0
19	35.01183827931086	36.0	36.0	36.0	36.0	36.0
20	35.00405829055956	36.0	36.0	36.0	36.0	36.0
21	35.001119802995746	36.0	36.0	36.0	36.0	36.0
22	34.990347037589046	36.0	36.0	36.0	36.0	36.0
23	34.93266234428984	36.0	36.0	36.0	36.0	36.0
24	34.90344286941736	36.0	36.0	36.0	32.0	36.0
25	34.87910036460554	36.0	36.0	36.0	32.0	36.0
26	34.8196107399748	36.0	36.0	36.0	32.0	36.0
27	34.81548187359618	36.0	36.0	36.0	32.0	36.0
28	34.79720925115075	36.0	36.0	36.0	32.0	36.0
29	34.76441900176114	36.0	36.0	36.0	32.0	36.0
30	34.751439203817895	36.0	36.0	36.0	32.0	36.0
31	34.7433461479718	36.0	36.0	36.0	32.0	36.0
32	34.70581465125049	36.0	36.0	36.0	32.0	36.0
33	34.68848448743497	36.0	36.0	36.0	32.0	36.0
34	34.68154706538506	36.0	36.0	36.0	32.0	36.0
35	34.64852988759262	36.0	36.0	36.0	32.0	36.0
36	34.62367525568352	36.0	36.0	36.0	32.0	36.0
37	34.615368662743855	36.0	36.0	36.0	32.0	36.0
38	34.579314191240925	36.0	36.0	36.0	32.0	36.0
39	34.57726676863364	36.0	36.0	36.0	32.0	36.0
40	34.562247510483225	36.0	36.0	36.0	32.0	36.0
41	34.55070890695791	36.0	36.0	36.0	32.0	36.0
42	34.48405710343809	36.0	36.0	36.0	32.0	36.0
43	34.48144073115101	36.0	36.0	36.0	32.0	36.0
44	34.419185620194966	36.0	36.0	36.0	32.0	36.0
45	34.39902590892607	36.0	36.0	36.0	32.0	36.0
46	34.37726828872821	36.0	36.0	36.0	32.0	36.0
47	34.36147486813179	36.0	36.0	36.0	32.0	36.0
48	34.311492349219286	36.0	36.0	36.0	32.0	36.0
49	34.301680500733625	36.0	36.0	36.0	32.0	36.0
50	33.70775566723827	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	2.0
18	4.0
19	7.0
20	23.0
21	82.0
22	298.0
23	819.0
24	2015.0
25	4905.0
26	9823.0
27	18125.0
28	29942.0
29	46573.0
30	67990.0
31	96521.0
32	140776.0
33	227155.0
34	475735.0
35	1642190.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.45438895709785	18.18056778169014	11.962528667068199	26.40251459414381
2	16.172873643758194	20.128586453832582	36.729478393870934	26.96906150853829
3	17.961808613094778	23.778392814422407	28.96677824912893	29.293020323353886
4	12.158223023931356	15.982093286031851	36.09037468883302	35.76930900120377
5	14.365074596831109	36.709306525621194	33.41953234652655	15.506086531021149
6	34.51247040786745	36.01363889219651	16.368118347538402	13.105772352397645
7	30.5147764049474	30.54210191437814	20.490368029371123	18.452753651303336
8	28.22860058957975	33.008069171566255	18.951315334683322	19.81201490417067
9	27.186498807798603	13.960844834300653	18.359720531199194	40.49293582670155
10	15.697354572421709	26.62072794499804	31.494204033650913	26.187713448929333
11	37.54493781182308	20.942097043595968	22.44293762000156	19.070027524579395
12	24.71853749159874	23.5307615439712	28.652335034033914	23.09836593039614
13	29.380081028630745	19.267788983899234	25.364661411942347	25.98746857552767
14	23.63652551703271	19.588538164256104	24.86125477654907	31.913681542162113
15	25.164620425636958	27.487493987232845	21.958245607558226	25.389639979571974
16	26.084884081176472	25.837758526338344	23.403781730371108	24.673575662114075
17	24.14232283478816	26.06535103688546	24.887132978596345	24.90519314973004
18	25.038020570558288	24.91054941443616	25.88391945212738	24.16751056287817
19	25.57468622714701	24.8720044184082	25.126765028849224	24.42654432559557
20	25.934892407250903	24.24623547529556	24.649606491576982	25.169265625876548
21	27.014686285055372	24.23475906139228	24.23631534868436	24.514239304867992
22	26.17721498829345	24.114444424739492	24.790706276513465	24.917634310453593
23	24.61249028669584	24.005677962390376	25.393246308401523	25.988585442512257
24	24.955111823225458	24.823007936729116	25.0909797656658	25.130900474379626
25	25.0584252737401	24.28500747937607	25.24554540088044	25.411021846003397
26	24.78297567995645	25.288979681659058	25.445884356006754	24.48216028237774
27	25.398427175898664	24.64274480861995	24.80772230790419	25.151105707577205
28	24.813625625395197	24.508723410185052	25.552825286035848	25.1248256783839
29	24.72314546277787	24.622780377087516	25.345206309427766	25.308867850706847
30	24.601040582726327	24.88476677374112	25.606442564357778	24.90775007917477
31	25.4017790084245	24.572214530680203	24.64666865741872	25.379337803476577
32	25.133745969342936	24.669810962229004	24.428650176427773	25.76779289200029
33	24.68067301713236	24.43737282117472	25.029552902409907	25.852401259283013
34	25.39084937418858	24.458529519601374	25.345462165925355	24.805158940284695
35	25.822678152678147	24.26163552242447	25.393092829736325	24.522593495161058
36	24.741066125277847	24.899023480411515	24.94372497756781	25.416185416742827
37	25.802767983240667	24.557074181342788	24.730045988277443	24.910111847139106
38	24.852566819544247	24.44163969802847	25.192297460127982	25.513496022299304
39	25.670114921311242	24.393243636003007	24.4916204190822	25.44502102360355
40	25.759036667861057	24.613758681969372	25.025679446670356	24.601525203499207
41	24.692947881309614	25.00975429315578	25.564355532584404	24.732942292950195
42	25.969561945808838	25.014341695371172	24.934933040117297	24.081163318702693
43	25.196339413198597	24.098163372777957	25.214544625390662	25.49095258863278
44	24.753830885817923	24.77735656753789	24.85730769202928	25.611504854614907
45	24.940624884633845	24.912466494048736	24.928427741154174	25.218480880163245
46	24.863642121645345	24.63269331692575	24.998715141424928	25.50494942000398
47	25.089696672690483	24.49485562934617	25.39057367867291	25.02487401929044
48	25.813557211769545	25.061601315705655	24.339577433794872	24.785264038729927
49	24.931314778641944	25.140075867717567	24.677022924993313	25.251586428647176
50	24.954676918544404	25.26633757289786	24.602117815762263	25.17686769279547
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	25.0
2	34.0
3	120.0
4	206.0
5	259.0
6	312.0
7	312.5
8	313.0
9	414.0
10	515.0
11	763.5
12	1012.0
13	1897.0
14	2782.0
15	3747.0
16	4712.0
17	4898.0
18	5084.0
19	5146.5
20	5209.0
21	6135.0
22	7061.0
23	8304.5
24	9548.0
25	12367.0
26	15186.0
27	19551.5
28	23917.0
29	28046.5
30	32176.0
31	36133.5
32	40091.0
33	46694.0
34	53297.0
35	62739.0
36	72181.0
37	86312.0
38	100443.0
39	109827.5
40	119212.0
41	131505.5
42	143799.0
43	147883.0
44	151967.0
45	168372.5
46	184778.0
47	196298.5
48	207819.0
49	215218.5
50	222618.0
51	216569.5
52	210521.0
53	208052.5
54	205584.0
55	204109.5
56	202635.0
57	196474.5
58	190314.0
59	173253.5
60	156193.0
61	137170.5
62	118148.0
63	102510.5
64	86873.0
65	73559.0
66	60245.0
67	51499.0
68	42753.0
69	38426.5
70	34100.0
71	25837.5
72	17575.0
73	15190.0
74	12805.0
75	9907.0
76	7009.0
77	6271.5
78	5534.0
79	4536.5
80	3539.0
81	2895.5
82	2252.0
83	1833.5
84	1415.0
85	996.5
86	578.0
87	416.0
88	254.0
89	177.0
90	100.0
91	96.0
92	92.0
93	72.0
94	52.0
95	56.5
96	61.0
97	47.5
98	34.0
99	33.0
100	32.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008469098287142967
2	7.238545544566639E-4
3	2.533490940598324E-4
4	0.0
5	0.0
6	1.8096363861416597E-4
7	0.0
8	3.6192727722833194E-5
9	0.001085781831684996
10	0.001085781831684996
11	3.6192727722833194E-5
12	2.533490940598324E-4
13	4.3431273267399836E-4
14	2.8954182178266556E-4
15	0.004162163688125818
16	6.514690990109975E-4
17	0.0
18	2.8954182178266556E-4
19	0.0
20	7.600472821794971E-4
21	0.0
22	1.0857818316849959E-4
23	8.324327376251636E-4
24	3.2573454950549877E-4
25	0.0019906000247558258
26	0.005863221891098978
27	0.006695654628724141
28	0.004813632787136815
29	0.0026420691237668235
30	0.004017392777234485
31	0.0076366655495178045
32	0.0035830800445604866
33	0.00521175279208798
34	0.0033659236782234868
35	0.003221152767332154
36	0.007781436460409137
37	0.003908814594065985
38	0.007564280094072139
39	0.005030789153473814
40	0.002750647306935323
41	0.0036192727722833195
42	0.0022801418465384915
43	0.0014839018366361609
44	0.0017372509306959934
45	0.0015924800198046606
46	0.001302938198021995
47	0.0022801418465384915
48	0.0020991782079243255
49	6.876618267338307E-4
50	0.0016286727475274937
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2762986.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.71512228877873
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.91251759517135	36.98087577897839
2	13.67919069824342	13.327668953529054
3	4.41000940134819	6.445024418440227
4	1.9093192864108166	3.7205089010330665
5	1.016410381444108	2.4757278013816975
6	0.5952595227169215	1.7398884265628936
7	0.4239433357850116	1.4456716012395736
8	0.2936766031161165	1.14451933073238
9	0.22413232843431188	0.9826770409691599
>10	1.3412703109183688	12.25789797916666
>50	0.10295016724535266	3.456741049362018
>100	0.07412423859683928	7.4544294814474625
>500	0.011513930903020405	3.9443468743535326
>1k	0.005682199666416617	4.624022362803837
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	3015	0.10912107408434209	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	2993	0.10832483407443975	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2990	0.10821625589127126	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2931	0.1060808849556241	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2887	0.10448840493581944	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	2794	0.10112248125759594	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2788	0.10090532489125895	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.238545544566639E-5
2	0.0	0.0	0.0	0.0	7.238545544566639E-5
3	0.0	0.0	0.0	0.0	7.238545544566639E-5
4	0.0	0.0	0.0	0.0	7.238545544566639E-5
5	0.0	0.0	0.0	0.0	7.238545544566639E-5
6	0.0	0.0	0.0	0.0	7.238545544566639E-5
7	0.0	0.0	0.0	0.0	7.238545544566639E-5
8	0.0	0.0	0.0	0.0	7.238545544566639E-5
9	0.0	0.0	0.0	0.0	7.238545544566639E-5
10	0.0	0.0	0.0	0.0	1.0857818316849959E-4
11	0.0	0.0	0.0	0.0	1.0857818316849959E-4
12	0.0	0.0	0.0	0.0	4.705054603968315E-4
13	0.0	0.0	0.0	0.0	5.066981881196648E-4
14	0.0	0.0	0.0	0.0	5.066981881196648E-4
15	0.0	0.0	0.0	0.0	5.790836435653311E-4
16	0.0	0.0	0.0	0.0	5.790836435653311E-4
17	0.0	0.0	0.0	0.0	5.790836435653311E-4
18	0.0	0.0	0.0	0.0	5.790836435653311E-4
19	0.0	0.0	0.0	0.0	5.790836435653311E-4
20	0.0	0.0	0.0	0.0	6.514690990109975E-4
21	0.0	0.0	0.0	3.6192727722833194E-5	6.514690990109975E-4
22	0.0	0.0	0.0	7.238545544566639E-5	6.876618267338307E-4
23	0.0	0.0	0.0	2.533490940598324E-4	6.876618267338307E-4
24	0.0	0.0	0.0	4.705054603968315E-4	6.876618267338307E-4
25	0.0	0.0	0.0	7.238545544566639E-4	6.876618267338307E-4
26	0.0	0.0	0.0	7.600472821794971E-4	6.876618267338307E-4
27	0.0	0.0	0.0	0.0013391309257448282	6.876618267338307E-4
28	0.0	0.0	0.0	0.002931610945549489	6.876618267338307E-4
29	0.0	0.0	0.0	0.006044185529713144	6.876618267338307E-4
30	0.0	3.6192727722833194E-5	0.0	0.012486491064377452	6.876618267338307E-4
31	0.0	3.6192727722833194E-5	0.0	0.03058285492579405	7.238545544566639E-4
32	0.0	3.6192727722833194E-5	0.0	0.049222109703053143	7.238545544566639E-4
33	0.0	3.6192727722833194E-5	0.0	0.06901953176744291	7.238545544566639E-4
34	0.0	3.6192727722833194E-5	0.0	0.09084374658431132	7.600472821794971E-4
35	0.0	3.6192727722833194E-5	0.0	0.11730063054970238	7.600472821794971E-4
36	0.0	3.6192727722833194E-5	0.0	0.1577641001438299	7.600472821794971E-4
37	0.0	3.6192727722833194E-5	0.0	0.21755448634195035	7.600472821794971E-4
38	0.0	3.6192727722833194E-5	0.0	0.29793853461436287	7.962400099023303E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3785	0.0	27.900394	1
GGTATCA	1590	0.0	23.384312	1
TCAACGC	5445	0.0	19.069178	4
AACGCAG	5640	0.0	18.448875	6
CAACGCA	5635	0.0	18.426207	5
TCGATCG	180	1.4551915E-11	18.332857	41
ATCAACG	5705	0.0	18.200119	3
TATCAAC	5930	0.0	17.69472	2
TAGGACG	400	0.0	17.048632	4
CGAGCCG	970	0.0	17.008944	15
CTAGCGG	910	0.0	16.924477	29
CGCAATA	900	0.0	16.868977	36
ACCCGTT	405	0.0	16.841509	30
ACGACGG	855	0.0	16.466515	6
CGGTCCA	1005	0.0	16.416594	10
CGACGGT	845	0.0	16.140717	7
TAGCGGC	955	0.0	16.127571	30
GTCCTAC	765	0.0	16.105034	1
ATACGAA	950	0.0	15.978534	40
GATATAC	470	0.0	15.915346	1
>>END_MODULE