FastQCFastQC Report
Thu 2 Feb 2017
SRR4064063_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064063_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1706573
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41090.2407749331555111No Hit
TATCAACGCAGAGTACTTTTTTTTT26510.1553405567766512No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGGG359.9367084E-519.0009087
TTCGCGC456.749836E-414.7815217
CGTCGTA1700.013.41398110
CGCGGTC1351.8189894E-1213.37257910
TAGGCCC500.001503756413.29517654
AGAACCG1800.013.1915935
CTAGACA655.4716802E-513.1490764
CGAACGT905.4298107E-712.6620734
CGCCAGT1900.012.50316518
AACCGCG1457.2759576E-1212.4488727
CGGTCCT1551.8189894E-1212.26044912
CGCATCG1800.012.14089413
ACCGGCG1103.808782E-812.0921968
GTATCAA25950.012.0733581
TCGAACG951.0471158E-611.9931883
CGACCAT2150.011.93220210
CCGTCGT1850.011.8127629
GTTCGCG658.007094E-411.6952716
TACCGTC2000.011.4005447
GACTGAT855.320734E-511.1770067
Produced by FastQC (version 0.11.5)