##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064063_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1706573
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.262900561534725	32.0	32.0	32.0	32.0	32.0
2	30.893436729633013	32.0	32.0	32.0	32.0	32.0
3	30.910517159242527	32.0	32.0	32.0	32.0	32.0
4	30.93939081422242	32.0	32.0	32.0	32.0	32.0
5	30.852591714506207	32.0	32.0	32.0	32.0	32.0
6	34.5119810286463	36.0	36.0	36.0	32.0	36.0
7	34.45056965040464	36.0	36.0	36.0	32.0	36.0
8	34.409752175851835	36.0	36.0	36.0	32.0	36.0
9	34.5381351984357	36.0	36.0	36.0	32.0	36.0
10	34.25546695043224	36.0	36.0	36.0	32.0	36.0
11	34.52295917022008	36.0	36.0	36.0	32.0	36.0
12	34.35141479444477	36.0	36.0	36.0	32.0	36.0
13	34.42309763485066	36.0	36.0	36.0	32.0	36.0
14	34.34127634739328	36.0	36.0	36.0	32.0	36.0
15	34.27410723127578	36.0	36.0	36.0	32.0	36.0
16	34.2774771427885	36.0	36.0	36.0	32.0	36.0
17	34.20332092444917	36.0	36.0	36.0	32.0	36.0
18	34.20266053664273	36.0	36.0	36.0	32.0	36.0
19	34.20689299549448	36.0	36.0	36.0	32.0	36.0
20	34.18599262967362	36.0	36.0	36.0	32.0	36.0
21	34.17395622689448	36.0	36.0	36.0	32.0	36.0
22	34.15500303825269	36.0	36.0	36.0	32.0	36.0
23	34.0969100061937	36.0	36.0	36.0	32.0	36.0
24	34.09149095878114	36.0	36.0	36.0	32.0	36.0
25	33.69680523481855	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	16.0
5	12.0
6	59.0
7	36.0
8	96.0
9	114.0
10	174.0
11	62.0
12	108.0
13	97.0
14	151.0
15	269.0
16	463.0
17	610.0
18	822.0
19	1047.0
20	1599.0
21	2342.0
22	3603.0
23	5708.0
24	8355.0
25	12420.0
26	17681.0
27	23837.0
28	32894.0
29	44596.0
30	58832.0
31	80581.0
32	113805.0
33	161888.0
34	351979.0
35	782314.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.054592691508205	17.38501418014826	11.41821413372132	26.142178994622213
2	16.21507904370507	19.7973466724408	38.26949458557799	25.718079698276142
3	18.9604283264061	24.098751339691976	28.540170835997092	28.400649497904833
4	12.541605332983302	15.610672529698002	36.175778429920214	35.67194370739848
5	14.339317913716327	36.844871870753224	33.7790758660862	15.036734349444252
6	33.635984840271384	35.73079116225563	17.2337890589517	13.399434938521287
7	29.80936271372406	30.622938367292395	21.054911228176294	18.512787690807258
8	27.658056631036576	33.55106084275376	19.494007755186978	19.296874771022683
9	27.459684842344956	14.253561323713743	18.680519157687876	39.60623467625343
10	15.752479711716106	26.798007201234096	31.972201195984145	25.47731189106565
11	36.59220988767576	21.073605855541576	23.248971914835735	19.08521234194693
12	24.94725719841027	23.540235222927024	29.27709232658202	22.23541525208069
13	29.47109492731953	19.901665875068165	25.36340234899526	25.26383684861705
14	23.44092301485662	19.893653451923573	25.452922770921	31.212500762298813
15	24.635490477633976	27.844206223237496	22.424188410394304	25.096114888734228
16	25.081740929336902	25.868893178561127	24.490673090229322	24.558692801872652
17	23.582649301974314	26.18213460001536	25.57250185141426	24.662714246596064
18	24.219611789522574	25.257138567765008	26.811903995346025	23.711345647366393
19	25.478885075205408	24.932752308588178	25.616986939844473	23.971375676361944
20	25.71532457174358	24.676073353389985	25.311904384900043	24.296697689966397
21	26.290465025996372	24.284528024827033	24.73596061077691	24.689046338399685
22	25.651477832512313	24.73440065681445	25.128723903354445	24.48539760731879
23	24.178057929468398	24.667176507294553	25.80417193515015	25.350593628086905
24	24.74234542778224	25.024660008562094	25.64341803552683	24.58957652812883
25	24.640232606282318	24.86337371767166	25.726322851467774	24.770070824578248
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	127.0
1	127.0
2	355.0
3	583.0
4	583.0
5	583.0
6	1562.5
7	2542.0
8	2542.0
9	2542.0
10	2838.5
11	3135.0
12	3135.0
13	3135.0
14	3827.5
15	4520.0
16	4520.0
17	4520.0
18	7954.5
19	11389.0
20	11389.0
21	11389.0
22	18834.0
23	26279.0
24	26279.0
25	26279.0
26	39446.0
27	52613.0
28	52613.0
29	52613.0
30	67272.0
31	81931.0
32	81931.0
33	81931.0
34	98310.5
35	114690.0
36	114690.0
37	114690.0
38	129721.0
39	144752.0
40	144752.0
41	144752.0
42	164032.0
43	183312.0
44	183312.0
45	183312.0
46	202669.5
47	222027.0
48	222027.0
49	222027.0
50	225396.5
51	228766.0
52	228766.0
53	228766.0
54	212168.0
55	195570.0
56	195570.0
57	195570.0
58	178460.5
59	161351.0
60	161351.0
61	161351.0
62	140613.0
63	119875.0
64	119875.0
65	119875.0
66	97555.0
67	75235.0
68	75235.0
69	75235.0
70	56743.0
71	38251.0
72	38251.0
73	38251.0
74	28819.5
75	19388.0
76	19388.0
77	19388.0
78	15340.0
79	11292.0
80	11292.0
81	11292.0
82	7815.0
83	4338.0
84	4338.0
85	4338.0
86	3321.5
87	2305.0
88	2305.0
89	2305.0
90	1654.5
91	1004.0
92	1004.0
93	1004.0
94	707.0
95	410.0
96	410.0
97	410.0
98	649.0
99	888.0
100	888.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03908417629951956
2	0.0065628601882251735
3	0.0014063271831911087
4	0.003574414923944068
5	0.007734799507551098
6	0.012305362852922202
7	0.023497383352484774
8	0.036212924967171045
9	0.05197550881210473
10	0.06047206887721768
11	0.05713204181713879
12	0.06603878064401582
13	0.06814827141880248
14	0.07060934398938691
15	0.06222997785620656
16	0.069437404670061
17	0.06562860188225174
18	0.07992626157802801
19	0.07676202541584802
20	0.08221154325071357
21	0.07822694956500542
22	0.08045363427172467
23	0.08584455514062392
24	0.08103960393138764
25	0.08051223123769097
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1706573.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.433563215992685
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.43859865688336	42.69692418563339
2	12.776005126641191	13.908869654177398
3	3.7554680834850216	6.132705279840745
4	1.6040272631647159	3.492516777186092
5	0.8292229603308551	2.2568780215661097
6	0.5336095026140122	1.7427759955916549
7	0.34943672800954884	1.3314760356868194
8	0.24905840669732582	1.084570922034664
9	0.20692291369642538	1.0137196353178595
>10	1.0921914926260345	10.808816205218607
>50	0.08621514609731619	3.238908668462436
>100	0.06940618454577606	7.751101037738012
>500	0.007459229993188964	2.6907595077210464
>1k	0.0023783052152196696	1.8499780738252691
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4109	0.2407749331555111	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2651	0.1553405567766512	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.9298482983148097E-4
2	0.0	0.0	0.0	0.0	2.9298482983148097E-4
3	0.0	0.0	0.0	0.0	2.9298482983148097E-4
4	0.0	0.0	0.0	0.0	2.9298482983148097E-4
5	0.0	0.0	0.0	5.8596965966296195E-5	4.6877572773036956E-4
6	0.0	0.0	0.0	5.8596965966296195E-5	5.273726936966658E-4
7	0.0	0.0	0.0	1.757908978988886E-4	5.273726936966658E-4
8	0.0	0.0	0.0	2.3438786386518478E-4	5.859696596629619E-4
9	0.0	0.0	0.0	2.3438786386518478E-4	5.859696596629619E-4
10	0.0	0.0	0.0	2.3438786386518478E-4	5.859696596629619E-4
11	0.0	0.0	0.0	2.3438786386518478E-4	5.859696596629619E-4
12	0.0	0.0	0.0	2.3438786386518478E-4	0.001464924149157405
13	0.0	0.0	0.0	2.3438786386518478E-4	0.001523521115123701
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGGG	35	9.9367084E-5	19.000908	7
TTCGCGC	45	6.749836E-4	14.78152	17
CGTCGTA	170	0.0	13.413981	10
CGCGGTC	135	1.8189894E-12	13.372579	10
TAGGCCC	50	0.0015037564	13.2951765	4
AGAACCG	180	0.0	13.191593	5
CTAGACA	65	5.4716802E-5	13.149076	4
CGAACGT	90	5.4298107E-7	12.662073	4
CGCCAGT	190	0.0	12.503165	18
AACCGCG	145	7.2759576E-12	12.448872	7
CGGTCCT	155	1.8189894E-12	12.260449	12
CGCATCG	180	0.0	12.140894	13
ACCGGCG	110	3.808782E-8	12.092196	8
GTATCAA	2595	0.0	12.073358	1
TCGAACG	95	1.0471158E-6	11.993188	3
CGACCAT	215	0.0	11.932202	10
CCGTCGT	185	0.0	11.812762	9
GTTCGCG	65	8.007094E-4	11.69527	16
TACCGTC	200	0.0	11.400544	7
GACTGAT	85	5.320734E-5	11.177006	7
>>END_MODULE