##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064063_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1706573
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3470258816939	32.0	32.0	32.0	32.0	32.0
2	31.406176588988576	32.0	32.0	32.0	32.0	32.0
3	31.49040093802023	32.0	32.0	32.0	32.0	32.0
4	31.568458542353593	32.0	32.0	32.0	32.0	32.0
5	31.519691803397805	32.0	32.0	32.0	32.0	32.0
6	35.11314722546296	36.0	36.0	36.0	36.0	36.0
7	35.126860087438395	36.0	36.0	36.0	36.0	36.0
8	35.08077181579692	36.0	36.0	36.0	36.0	36.0
9	35.18920843116585	36.0	36.0	36.0	36.0	36.0
10	35.04718227699606	36.0	36.0	36.0	36.0	36.0
11	35.19222558894346	36.0	36.0	36.0	36.0	36.0
12	35.10090514733328	36.0	36.0	36.0	36.0	36.0
13	35.14007663311209	36.0	36.0	36.0	36.0	36.0
14	35.085613097125055	36.0	36.0	36.0	36.0	36.0
15	35.05504130207146	36.0	36.0	36.0	36.0	36.0
16	35.07376010284939	36.0	36.0	36.0	36.0	36.0
17	35.032445140055536	36.0	36.0	36.0	36.0	36.0
18	35.033716694217006	36.0	36.0	36.0	36.0	36.0
19	35.031251519858806	36.0	36.0	36.0	36.0	36.0
20	35.02498398837905	36.0	36.0	36.0	36.0	36.0
21	35.01779238274601	36.0	36.0	36.0	36.0	36.0
22	35.00517997179142	36.0	36.0	36.0	36.0	36.0
23	34.95644956295453	36.0	36.0	36.0	36.0	36.0
24	34.92389133075468	36.0	36.0	36.0	36.0	36.0
25	34.90644877189549	36.0	36.0	36.0	32.0	36.0
26	34.84791450468278	36.0	36.0	36.0	32.0	36.0
27	34.832374589308515	36.0	36.0	36.0	32.0	36.0
28	34.81672275372926	36.0	36.0	36.0	32.0	36.0
29	34.78767096397283	36.0	36.0	36.0	32.0	36.0
30	34.77037665543754	36.0	36.0	36.0	32.0	36.0
31	34.76047962788583	36.0	36.0	36.0	32.0	36.0
32	34.71485368630583	36.0	36.0	36.0	32.0	36.0
33	34.70281728352669	36.0	36.0	36.0	32.0	36.0
34	34.69571708916056	36.0	36.0	36.0	32.0	36.0
35	34.6558576750013	36.0	36.0	36.0	32.0	36.0
36	34.62828311475688	36.0	36.0	36.0	32.0	36.0
37	34.611707790994	36.0	36.0	36.0	32.0	36.0
38	34.57199545521932	36.0	36.0	36.0	32.0	36.0
39	34.56569745331726	36.0	36.0	36.0	32.0	36.0
40	34.545937384454106	36.0	36.0	36.0	32.0	36.0
41	34.52772720534076	36.0	36.0	36.0	32.0	36.0
42	34.47111140279379	36.0	36.0	36.0	32.0	36.0
43	34.46477121107623	36.0	36.0	36.0	32.0	36.0
44	34.38900123229419	36.0	36.0	36.0	32.0	36.0
45	34.367964921512296	36.0	36.0	36.0	32.0	36.0
46	34.33487228498283	36.0	36.0	36.0	32.0	36.0
47	34.30555622290989	36.0	36.0	36.0	32.0	36.0
48	34.2666677604767	36.0	36.0	36.0	32.0	36.0
49	34.236703029990515	36.0	36.0	36.0	32.0	36.0
50	33.652607887268815	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	1.0
17	2.0
18	0.0
19	3.0
20	14.0
21	47.0
22	160.0
23	500.0
24	1249.0
25	2799.0
26	5712.0
27	10997.0
28	18526.0
29	28817.0
30	41530.0
31	59884.0
32	87190.0
33	141370.0
34	295223.0
35	1012548.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.45061347482195	18.118970222576177	12.040655709268549	26.38976059333332
2	15.610110824831372	20.489145752847676	37.849788493507724	26.050954928813226
3	18.06878390588798	24.74794352622407	28.911753572387056	28.271518995500895
4	12.225085009548376	16.284624214727412	36.34945589787252	35.140834877851695
5	14.01938270440233	37.21112428240691	33.622353101801096	15.147139911389667
6	33.69854954590175	36.080697586795495	16.994859276126544	13.225893591176213
7	29.759816896200746	30.70393121184971	21.07738725504271	18.45886463690683
8	27.61276546622969	33.80078086316847	19.275589148545066	19.31086452205678
9	27.58001779019523	14.132071690502169	18.46060901533965	39.82730150396295
10	15.698224963024945	26.99694589571042	32.014361087476765	25.290468053787862
11	36.873547161475074	21.05822604717173	23.145391377925232	18.92283541342796
12	25.023087218119127	23.60398506479656	29.190857461624827	22.182070255459482
13	29.65233711890597	19.85055377257383	25.410019061660044	25.087090046860162
14	23.46225266924142	19.879219792203195	25.45654414612694	31.201983392428446
15	24.784032403050443	27.835530610498875	22.365230476775352	25.015206509675323
16	25.122263357557472	25.89352159487906	24.43685022870543	24.547364818858032
17	23.65582954845764	26.186632508541972	25.57154015679376	24.585997786206626
18	24.37575040915427	25.23484254079384	26.69355883061277	23.69584821943912
19	25.654396266670105	24.8431798698327	25.60318251841556	23.899241345081634
20	25.90572714535758	24.54995643274734	25.345359106083183	24.198957315811896
21	26.470769196512546	24.322604424188125	24.751182633265614	24.455443746033716
22	25.833468594663106	24.736650585705974	25.052546829230277	24.377333990400647
23	24.30522472413787	24.68065856498369	25.75744524508089	25.25667146579755
24	24.923632967119254	25.058289741767215	25.614437456381577	24.403639834731955
25	24.794541017084228	24.748483046154657	25.792756710195157	24.664219226565955
26	24.264660208966944	25.469296626408592	26.06713194920568	24.198911215418782
27	24.877613710546022	25.27275412222338	25.252068290201713	24.597563877028882
28	24.225238877110574	25.165921878717345	25.819940627918943	24.788898616253135
29	24.5264809136195	25.018355951342226	25.550839685889095	24.904323449149178
30	24.342889020540596	25.205168638219348	25.868857319825167	24.583085021414888
31	25.057942231814636	24.842464521187665	25.087946199136095	25.0116470478616
32	24.703403288317904	25.22159052953193	25.06601130015013	25.008994882000042
33	24.30599545622086	25.000981535989553	25.64973287571293	25.043290132076656
34	24.89522610768506	24.986522374031075	25.813697018280347	24.304554500003515
35	25.236239067021227	25.03800100085905	25.664709873931606	24.061050058188115
36	24.438236650557265	25.305765102783134	25.517021524714657	24.73897672194494
37	25.009463648456226	25.168499821274747	25.15924126736711	24.662795262901913
38	24.58706574951683	25.295966001854147	25.431276465824293	24.68569178280473
39	25.16374128847122	24.8893204406907	25.117916581453414	24.829021689384664
40	25.51285056312773	25.05244529867451	25.45290470777128	23.981799430426477
41	24.587393475294004	25.12825741607673	25.86853197337988	24.415817135249387
42	25.368596908714068	25.59314495596057	25.341876159876804	23.696381975448556
43	24.669303565673435	24.950631390817733	25.615188538278982	24.76487650522985
44	24.324441519767063	25.430942292378017	25.231124062509707	25.013492125345216
45	24.47527328448248	25.55276565947186	25.237862242939723	24.734098813105938
46	24.677963082436648	24.987416198326574	25.204519738256927	25.130100980979854
47	24.986053521661578	25.019337553998653	25.432575223085053	24.562033701254716
48	25.144767452081123	25.586130920640127	24.71998734273643	24.549114284542316
49	24.740853855051608	25.481112362487007	24.948932534692474	24.82910124776891
50	24.557618317103785	25.89246893454544	24.785328128314422	24.764584620036352
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	22.0
1	29.0
2	36.0
3	95.0
4	154.0
5	205.5
6	257.0
7	255.5
8	254.0
9	337.5
10	421.0
11	661.0
12	901.0
13	1629.5
14	2358.0
15	3271.0
16	4184.0
17	4434.0
18	4684.0
19	4800.0
20	4916.0
21	5417.5
22	5919.0
23	6823.5
24	7728.0
25	9151.5
26	10575.0
27	13536.5
28	16498.0
29	20032.0
30	23566.0
31	27368.0
32	31170.0
33	36985.0
34	42800.0
35	49770.0
36	56740.0
37	63951.5
38	71163.0
39	75206.0
40	79249.0
41	84954.0
42	90659.0
43	92151.5
44	93644.0
45	101895.0
46	110146.0
47	115993.0
48	121840.0
49	126095.5
50	130351.0
51	127755.0
52	125159.0
53	123080.5
54	121002.0
55	120536.5
56	120071.0
57	115941.0
58	111811.0
59	101576.0
60	91341.0
61	80083.0
62	68825.0
63	59615.5
64	50406.0
65	42400.5
66	34395.0
67	29448.0
68	24501.0
69	21595.0
70	18689.0
71	14616.0
72	10543.0
73	9018.0
74	7493.0
75	5820.5
76	4148.0
77	3617.5
78	3087.0
79	2569.5
80	2052.0
81	1630.0
82	1208.0
83	1025.0
84	842.0
85	615.0
86	388.0
87	274.0
88	160.0
89	125.0
90	90.0
91	67.0
92	44.0
93	38.0
94	32.0
95	32.5
96	33.0
97	19.5
98	6.0
99	9.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008555157031079244
2	3.515817957977772E-4
3	6.445666256292582E-4
4	0.0
5	0.0
6	2.3438786386518478E-4
7	0.0
8	0.0
9	8.789544894944429E-4
10	9.961484214270355E-4
11	0.0
12	5.8596965966296195E-5
13	3.515817957977772E-4
14	1.1719393193259239E-4
15	0.003925996719741845
16	6.445666256292582E-4
17	0.0
18	2.3438786386518478E-4
19	0.0
20	9.375514554607391E-4
21	0.0
22	0.0
23	8.203575235281467E-4
24	7.031635915955544E-4
25	0.0016407150470562934
26	0.004863548175202584
27	0.005332323902932954
28	0.002754057400415921
29	0.0024610725705844403
30	0.0029298482983148096
31	0.007734799507551098
32	0.002754057400415921
33	0.0037502058218429565
34	0.002402475604618144
35	0.0022852816726855515
36	0.0067972480520903595
37	0.002519669536550736
38	0.007441814677719617
39	0.004101787617640734
40	0.0019336998768877745
41	0.002578266502517033
42	0.001992296842854071
43	0.0013477302172248125
44	0.0012891332512585164
45	0.0010547453873933316
46	8.203575235281467E-4
47	0.0028712513323485137
48	0.002519669536550736
49	4.1017876176407334E-4
50	8.789544894944429E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1706573.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.08820181997134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.22025581953778	46.017622082750705
2	12.606563205678198	14.6458517549532
3	3.660553344007396	6.3790488425841785
4	1.4645474166156964	3.4029170364516097
5	0.7723992350762546	2.2433641326350484
6	0.46712432296086165	1.6280647168300804
7	0.31605532270302433	1.2851359760014611
8	0.2255426755192553	1.048109476366305
9	0.18588127968335136	0.9717758359920949
>10	0.9462412823996064	9.912995668634359
>50	0.07315229335703721	2.9348266423867813
>100	0.055089664989426725	6.5528171742541375
>500	0.005376758246534629	2.120930739219792
>1k	0.001217379225630482	0.856539920940226
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.9298482983148097E-4
2	0.0	0.0	0.0	0.0	2.9298482983148097E-4
3	0.0	0.0	0.0	0.0	3.515817957977772E-4
4	0.0	0.0	0.0	0.0	3.515817957977772E-4
5	0.0	0.0	0.0	5.8596965966296195E-5	4.101787617640734E-4
6	0.0	0.0	0.0	5.8596965966296195E-5	4.6877572773036956E-4
7	0.0	0.0	0.0	5.8596965966296195E-5	5.273726936966658E-4
8	0.0	0.0	0.0	1.1719393193259239E-4	5.273726936966658E-4
9	0.0	0.0	0.0	1.1719393193259239E-4	5.273726936966658E-4
10	0.0	0.0	0.0	1.1719393193259239E-4	5.273726936966658E-4
11	0.0	0.0	0.0	1.1719393193259239E-4	5.273726936966658E-4
12	0.0	0.0	0.0	1.1719393193259239E-4	0.0011133423533596278
13	0.0	0.0	0.0	1.1719393193259239E-4	0.0011719393193259239
14	0.0	0.0	0.0	1.1719393193259239E-4	0.0011719393193259239
15	0.0	0.0	0.0	1.1719393193259239E-4	0.001464924149157405
16	0.0	0.0	0.0	1.1719393193259239E-4	0.0015821180810899973
17	0.0	0.0	0.0	1.1719393193259239E-4	0.0015821180810899973
18	0.0	0.0	0.0	1.1719393193259239E-4	0.0016993120130225896
19	0.0	0.0	0.0	1.1719393193259239E-4	0.0016993120130225896
20	0.0	0.0	0.0	2.3438786386518478E-4	0.0019922968428540706
21	0.0	0.0	0.0	2.3438786386518478E-4	0.002050893808820367
22	0.0	0.0	0.0	2.9298482983148097E-4	0.002109490774786663
23	0.0	0.0	0.0	5.859696596629619E-4	0.002109490774786663
24	0.0	0.0	0.0	9.375514554607391E-4	0.002109490774786663
25	0.0	0.0	0.0	0.0010547453873933316	0.002109490774786663
26	0.0	0.0	0.0	0.0011719393193259239	0.0021680877407529594
27	0.0	0.0	0.0	0.0012305362852922201	0.0022266847067192556
28	0.0	0.0	0.0	0.0022266847067192556	0.0023438786386518477
29	0.0	0.0	0.0	0.004394772447472215	0.0023438786386518477
30	0.0	0.0	0.0	0.0092583206226748	0.0023438786386518477
31	0.0	0.0	0.0	0.02173947437349589	0.002402475604618144
32	0.0	0.0	0.0	0.03615432800120475	0.0024610725705844403
33	0.0	0.0	0.0	0.05004180893521695	0.0024610725705844403
34	0.0	0.0	0.0	0.06767949569107211	0.0026368634684833287
35	0.0	0.0	0.0	0.08801264288137689	0.0026368634684833287
36	0.0	0.0	0.0	0.11824867731998573	0.0026368634684833287
37	0.0	0.0	0.0	0.1619034169648764	0.0026368634684833287
38	0.0	0.0	0.0	0.21973862237361075	0.0028126543663822175
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3175	0.0	23.489405	1
ATTATAC	230	0.0	21.999018	3
CGTATAC	75	5.4762088E-5	20.532417	3
CGGATCG	65	4.9338164E-4	20.307383	26
GGCGTAA	55	0.004485513	19.99911	6
TAGGACG	960	0.0	19.707455	4
CCGTATA	70	8.1292336E-4	18.85575	2
AACGCAG	4015	0.0	18.574514	6
GTATTAG	300	0.0	18.333054	1
GTCCTAA	535	0.0	18.093182	1
GTATTAC	160	1.1023076E-9	17.874725	1
AGGACGT	1015	0.0	17.555868	5
GGACGTG	975	0.0	17.373585	6
TTAGGAC	540	0.0	17.110348	3
ATCAACG	4395	0.0	16.968527	3
TCAACGC	4410	0.0	16.960695	4
TATCAAC	4455	0.0	16.93702	2
GTAGGAC	1150	0.0	16.834032	3
TAACATA	145	1.2507371E-7	16.68891	4
TGTAGGA	1200	0.0	16.6821	2
>>END_MODULE