FastQCFastQC Report
Thu 2 Feb 2017
SRR4064062_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064062_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2278522
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT83910.3682650419877447No Hit
TATCAACGCAGAGTACTTTTTTTTT58910.25854479351088117No Hit
GTACTTTTTTTTTTTTTTTTTTTTT45780.20091971901083247No Hit
GTCCTAAAGTGTGTATTTCTCATTT40620.17827345972520783No Hit
CTGTAGGACGTGGAATATGGCAAGA34730.1524233691840588No Hit
GTCCTACAGTGGACATTTCTAAATT34690.15224781678649582No Hit
CTTTAGGACGTGAAATATGGCGAGG34530.15154560719624388No Hit
GTACATGGGGTGGTATCAACGCAAA32690.14347019690834673No Hit
GAGTACTTTTTTTTTTTTTTTTTTT30990.13600922001192No Hit
GGTATCAACGCAGAGTACTTTTTTT29140.12788992162463209No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27280.11972673513795346No Hit
GTCCTACAGTGTGCATTTCTCATTT23310.10230315967982753No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT400.00528005914.25001711
CGAACGA1351.8189894E-1213.372148516
CGTCTTA801.9980744E-613.06395115
ATCGAGC957.392555E-813.00001511
GCTCGTA1403.6379788E-1212.8931569
CCGATAA1700.012.8532389
ATTCCGA1700.012.8515456
AATTCCG1850.012.8361615
CCAACGA2550.012.66974319
TCCAACG3150.012.66918618
CCGTCGT2250.012.666969
TCTTATA2300.012.3872372
CAATCGG1001.439821E-712.35245618
GTATCGA1001.4431316E-712.3502859
TCCGATA1700.012.2941328
TACCGTC2800.012.2134957
CGTCGTA2450.012.02068610
ACCGTCG2450.012.0204238
CGATAAC1750.011.94313310
TATCGAG1052.72099E-711.762176510
Produced by FastQC (version 0.11.5)