##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064062_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2278522
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.254266142701276	32.0	32.0	32.0	32.0	32.0
2	30.882963166473704	32.0	32.0	32.0	32.0	32.0
3	30.9095518937276	32.0	32.0	32.0	32.0	32.0
4	30.932383360792656	32.0	32.0	32.0	32.0	32.0
5	30.859641469338456	32.0	32.0	32.0	32.0	32.0
6	34.51989184216786	36.0	36.0	36.0	32.0	36.0
7	34.44538784352313	36.0	36.0	36.0	32.0	36.0
8	34.40385170737873	36.0	36.0	36.0	32.0	36.0
9	34.51991554174153	36.0	36.0	36.0	32.0	36.0
10	34.24649443806116	36.0	36.0	36.0	32.0	36.0
11	34.51775580837052	36.0	36.0	36.0	32.0	36.0
12	34.345776779859925	36.0	36.0	36.0	32.0	36.0
13	34.41534468396618	36.0	36.0	36.0	32.0	36.0
14	34.326978190247885	36.0	36.0	36.0	32.0	36.0
15	34.271494416117115	36.0	36.0	36.0	32.0	36.0
16	34.26265886394777	36.0	36.0	36.0	32.0	36.0
17	34.2049846347764	36.0	36.0	36.0	32.0	36.0
18	34.198138091271446	36.0	36.0	36.0	32.0	36.0
19	34.19288644129835	36.0	36.0	36.0	32.0	36.0
20	34.16268089577366	36.0	36.0	36.0	32.0	36.0
21	34.14810697460898	36.0	36.0	36.0	32.0	36.0
22	34.12171311051638	36.0	36.0	36.0	32.0	36.0
23	34.087278507734396	36.0	36.0	36.0	32.0	36.0
24	34.066704205621015	36.0	36.0	36.0	32.0	36.0
25	33.68348297712289	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	8.0
4	23.0
5	36.0
6	90.0
7	35.0
8	127.0
9	151.0
10	194.0
11	63.0
12	146.0
13	137.0
14	233.0
15	401.0
16	665.0
17	805.0
18	1173.0
19	1509.0
20	2219.0
21	3439.0
22	5284.0
23	7952.0
24	11824.0
25	17061.0
26	24339.0
27	32211.0
28	44100.0
29	59243.0
30	78028.0
31	106089.0
32	150765.0
33	212959.0
34	467580.0
35	1049632.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.136952470017974	17.587037317544887	11.623869705810684	25.65214050662645
2	15.357892759454913	20.12739686585978	40.33779086302972	24.17691951165559
3	18.813024472455318	24.976475545100243	29.152680734524772	27.057819247919667
4	12.002689570147176	16.07891570997761	37.24405084024928	34.67434387962594
5	13.486295471444034	37.84478588841319	34.388565499333275	14.280353140809499
6	32.395901872126856	36.75831606466653	17.81762093151161	13.028161131695
7	28.954229285969834	31.190021005406095	21.343336325744026	18.512413382880045
8	26.88993429732382	35.06222326616718	19.67721612075419	18.370626315754812
9	27.7799147269462	14.008667817106424	18.83805584462916	39.37336161131822
10	15.3595778671859	27.440521201933255	32.98443964907751	24.21546128180334
11	36.17122742674065	20.993065326103565	24.304995670220073	18.53071157693571
12	25.107310085083924	23.47352666568879	30.59766908529735	20.821494163929934
13	29.91657147725621	20.479008045097164	25.5471689329871	24.057251544659533
14	22.75797342871159	20.76115221595024	26.282439506203204	30.19843484913497
15	23.92448799990163	29.543270793114896	22.9919179605366	23.54032324644687
16	23.77333962823483	26.256502596646868	26.33524742256722	23.634910352551085
17	22.437093584614985	26.829355053614883	26.739942022060774	23.993609339709355
18	22.92277378713356	25.734194106066905	28.877715865936608	22.46531624086293
19	25.084405707833053	24.937181598216092	26.284334906739765	23.69407778721109
20	25.388449920651894	25.27033918294075	26.443057139857785	22.898153756549565
21	25.65302199045047	24.662843399021682	25.336915029728637	24.347219580799216
22	25.058560867504635	25.899254663895977	25.804292282088348	23.237892186511036
23	23.331962885460428	25.90682360051796	26.659515739945217	24.101697774076396
24	24.35706203969191	25.49245938044639	26.841276283240568	23.30920229662113
25	24.020604927139146	25.622546653390355	26.976414052215958	23.38043436725454
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	566.0
1	566.0
2	961.0
3	1356.0
4	1356.0
5	1356.0
6	3672.5
7	5989.0
8	5989.0
9	5989.0
10	5931.0
11	5873.0
12	5873.0
13	5873.0
14	6280.0
15	6687.0
16	6687.0
17	6687.0
18	11941.5
19	17196.0
20	17196.0
21	17196.0
22	29105.0
23	41014.0
24	41014.0
25	41014.0
26	64579.0
27	88144.0
28	88144.0
29	88144.0
30	116156.5
31	144169.0
32	144169.0
33	144169.0
34	161031.0
35	177893.0
36	177893.0
37	177893.0
38	193843.0
39	209793.0
40	209793.0
41	209793.0
42	231253.5
43	252714.0
44	252714.0
45	252714.0
46	275031.5
47	297349.0
48	297349.0
49	297349.0
50	294691.0
51	292033.0
52	292033.0
53	292033.0
54	263251.5
55	234470.0
56	234470.0
57	234470.0
58	212251.5
59	190033.0
60	190033.0
61	190033.0
62	163732.5
63	137432.0
64	137432.0
65	137432.0
66	112137.0
67	86842.0
68	86842.0
69	86842.0
70	65424.0
71	44006.0
72	44006.0
73	44006.0
74	33211.0
75	22416.0
76	22416.0
77	22416.0
78	17544.5
79	12673.0
80	12673.0
81	12673.0
82	8661.0
83	4649.0
84	4649.0
85	4649.0
86	3610.5
87	2572.0
88	2572.0
89	2572.0
90	1800.5
91	1029.0
92	1029.0
93	1029.0
94	745.0
95	461.0
96	461.0
97	461.0
98	812.0
99	1163.0
100	1163.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.039806506147406086
2	0.005705452920796903
3	0.0018433001744113072
4	0.003993817044557832
5	0.00983093426352697
6	0.01364919891052182
7	0.023567909372830282
8	0.03673433919005391
9	0.049681328510323794
10	0.05810784359334692
11	0.05573788622624666
12	0.06477883470074021
13	0.06508605139647544
14	0.06815821835382761
15	0.060302248562884186
16	0.06789488975748313
17	0.06359385601719009
18	0.07934968369846769
19	0.07553141905147284
20	0.08097354337592527
21	0.07728694302710265
22	0.08035910998445484
23	0.08312406024607179
24	0.0783841455118713
25	0.07834025741248055
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2278522.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.05045920892527
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.58926059991146	29.097487064179084
2	16.22515501396956	13.970007481747714
3	6.651080245615669	8.58996176427498
4	3.3117713375998634	5.702931075145232
5	1.8030790278501603	3.881169006946598
6	1.1089767260443193	2.8645174384931082
7	0.7194427334745026	2.1680638035421236
8	0.47775854713851473	1.6454179876241621
9	0.3305797909692814	1.2808450625776282
>10	1.5672487736128453	11.75061895581749
>50	0.10613556355824716	3.170740774007582
>100	0.09324321282073811	8.174860269200305
>500	0.010538666828941856	3.0445980716158765
>1k	0.00552512629866853	4.029687644440195
>5k	2.046343073580937E-4	0.6290936003879897
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	8391	0.3682650419877447	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5891	0.25854479351088117	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4578	0.20091971901083247	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4062	0.17827345972520783	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3473	0.1524233691840588	No Hit
GTCCTACAGTGGACATTTCTAAATT	3469	0.15224781678649582	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3453	0.15154560719624388	No Hit
GTACATGGGGTGGTATCAACGCAAA	3269	0.14347019690834673	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	3099	0.13600922001192	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2914	0.12788992162463209	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2728	0.11972673513795346	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2331	0.10230315967982753	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.388809939074541E-5
2	0.0	0.0	0.0	0.0	4.388809939074541E-5
3	0.0	0.0	0.0	0.0	4.388809939074541E-5
4	0.0	0.0	0.0	0.0	4.388809939074541E-5
5	0.0	4.388809939074541E-5	0.0	0.0	8.777619878149081E-5
6	0.0	4.388809939074541E-5	0.0	0.0	1.7555239756298163E-4
7	0.0	4.388809939074541E-5	0.0	4.388809939074541E-5	1.7555239756298163E-4
8	0.0	4.388809939074541E-5	0.0	4.388809939074541E-5	2.1944049695372703E-4
9	0.0	4.388809939074541E-5	0.0	4.388809939074541E-5	2.633285963444724E-4
10	0.0	4.388809939074541E-5	0.0	8.777619878149081E-5	2.633285963444724E-4
11	0.0	4.388809939074541E-5	0.0	8.777619878149081E-5	2.633285963444724E-4
12	0.0	4.388809939074541E-5	0.0	8.777619878149081E-5	3.0721669573521783E-4
13	0.0	4.388809939074541E-5	0.0	8.777619878149081E-5	4.3888099390745406E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACAT	40	0.005280059	14.250017	11
CGAACGA	135	1.8189894E-12	13.3721485	16
CGTCTTA	80	1.9980744E-6	13.063951	15
ATCGAGC	95	7.392555E-8	13.000015	11
GCTCGTA	140	3.6379788E-12	12.893156	9
CCGATAA	170	0.0	12.853238	9
ATTCCGA	170	0.0	12.851545	6
AATTCCG	185	0.0	12.836161	5
CCAACGA	255	0.0	12.669743	19
TCCAACG	315	0.0	12.669186	18
CCGTCGT	225	0.0	12.66696	9
TCTTATA	230	0.0	12.387237	2
CAATCGG	100	1.439821E-7	12.352456	18
GTATCGA	100	1.4431316E-7	12.350285	9
TCCGATA	170	0.0	12.294132	8
TACCGTC	280	0.0	12.213495	7
CGTCGTA	245	0.0	12.020686	10
ACCGTCG	245	0.0	12.020423	8
CGATAAC	175	0.0	11.943133	10
TATCGAG	105	2.72099E-7	11.7621765	10
>>END_MODULE