FastQCFastQC Report
Thu 2 Feb 2017
SRR4064062_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064062_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2278522
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23430.1028298168725165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC17300.020.3448734
TGTAGGA28900.019.029322
GTCCTAC24400.018.9351061
GATATAC13150.018.9056221
CTGTAGG29500.018.7938961
GTATCGA951.59944E-518.5245429
GTATCAA63950.018.4056661
AGGACCT21300.018.3832155
GTAGGAC28600.018.3828543
TTAGGAC15750.018.296663
CGTCGTA4000.018.14826210
TCCTACA26550.018.0623022
ATACACT13850.017.947744
TAGGACG28050.017.8022174
TAGGACA8700.017.6994534
ACTGTTC13950.017.5033158
CCTACAG26700.017.4664923
AGGACGT28550.017.3363445
GTCCTAA14350.017.3246631
GGACGTG28450.017.1653166
Produced by FastQC (version 0.11.5)