##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064062_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2278522
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28614689697971	32.0	32.0	32.0	32.0	32.0
2	31.436198553272693	32.0	32.0	32.0	32.0	32.0
3	31.503097622054998	32.0	32.0	32.0	32.0	32.0
4	31.58162440388989	32.0	32.0	32.0	32.0	32.0
5	31.53514120118217	32.0	32.0	32.0	32.0	32.0
6	35.12895376915387	36.0	36.0	36.0	36.0	36.0
7	35.14792132794856	36.0	36.0	36.0	36.0	36.0
8	35.09607675501926	36.0	36.0	36.0	36.0	36.0
9	35.19898469270869	36.0	36.0	36.0	36.0	36.0
10	35.068522928459764	36.0	36.0	36.0	36.0	36.0
11	35.20377595651918	36.0	36.0	36.0	36.0	36.0
12	35.12066374605995	36.0	36.0	36.0	36.0	36.0
13	35.15304219138547	36.0	36.0	36.0	36.0	36.0
14	35.103563187013336	36.0	36.0	36.0	36.0	36.0
15	35.07578070345601	36.0	36.0	36.0	36.0	36.0
16	35.087833692191694	36.0	36.0	36.0	36.0	36.0
17	35.061828676659694	36.0	36.0	36.0	36.0	36.0
18	35.05246295624971	36.0	36.0	36.0	36.0	36.0
19	35.04244286427781	36.0	36.0	36.0	36.0	36.0
20	35.03661715796468	36.0	36.0	36.0	36.0	36.0
21	35.02752003272297	36.0	36.0	36.0	36.0	36.0
22	35.006291797928654	36.0	36.0	36.0	36.0	36.0
23	34.9701341483646	36.0	36.0	36.0	36.0	36.0
24	34.947297414727615	36.0	36.0	36.0	36.0	36.0
25	34.91944778237823	36.0	36.0	36.0	36.0	36.0
26	34.86676758003653	36.0	36.0	36.0	32.0	36.0
27	34.86043496617544	36.0	36.0	36.0	32.0	36.0
28	34.8190607771178	36.0	36.0	36.0	32.0	36.0
29	34.78764084788297	36.0	36.0	36.0	32.0	36.0
30	34.76163890451793	36.0	36.0	36.0	32.0	36.0
31	34.75474408410364	36.0	36.0	36.0	32.0	36.0
32	34.708107711929046	36.0	36.0	36.0	32.0	36.0
33	34.69981417778718	36.0	36.0	36.0	32.0	36.0
34	34.6699105823863	36.0	36.0	36.0	32.0	36.0
35	34.63567654821854	36.0	36.0	36.0	32.0	36.0
36	34.59340791969531	36.0	36.0	36.0	32.0	36.0
37	34.56953849907967	36.0	36.0	36.0	32.0	36.0
38	34.52407525580179	36.0	36.0	36.0	32.0	36.0
39	34.489159639450484	36.0	36.0	36.0	32.0	36.0
40	34.48895775419329	36.0	36.0	36.0	32.0	36.0
41	34.46004295767168	36.0	36.0	36.0	32.0	36.0
42	34.393293547308296	36.0	36.0	36.0	32.0	36.0
43	34.3892593532123	36.0	36.0	36.0	32.0	36.0
44	34.3073834705129	36.0	36.0	36.0	32.0	36.0
45	34.26464348380222	36.0	36.0	36.0	32.0	36.0
46	34.20264013250695	36.0	36.0	36.0	32.0	36.0
47	34.17389957173992	36.0	36.0	36.0	32.0	36.0
48	34.10438126118598	36.0	36.0	36.0	32.0	36.0
49	34.06943097323616	36.0	36.0	36.0	32.0	36.0
50	33.50453539619104	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	1.0
17	5.0
18	4.0
19	4.0
20	14.0
21	72.0
22	269.0
23	723.0
24	1901.0
25	4243.0
26	8802.0
27	16044.0
28	26899.0
29	40704.0
30	56961.0
31	81199.0
32	115520.0
33	185486.0
34	384750.0
35	1354920.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.2712215320911	18.524731698174655	12.168268511190966	26.035778258543278
2	14.73898201537677	20.899871933401858	39.730270151178274	24.630875900043097
3	18.16654107305949	25.472314619227536	29.498844202337228	26.86230010537574
4	11.694861844651928	16.71912757480507	37.34793870763591	34.23807187290709
5	13.030815590106219	38.247600857046805	34.24768336667366	14.473900186173317
6	32.36807098652325	37.215961083459106	17.519509400193193	12.896458529824443
7	28.81592928044113	31.305482810998893	21.46537161606147	18.41321629249851
8	26.719821006775447	35.32421455662925	19.55056830699901	18.40539612959629
9	27.63128039004591	13.937013852528482	18.721124107472413	39.71058164995319
10	15.270555664789404	27.643103169810264	32.94524107130318	24.14110009409716
11	36.25639778768869	20.905130606594977	24.278326037668275	18.560145568048057
12	24.978033967693037	23.613945555839365	30.56324330112819	20.84477717533941
13	29.894918216326367	20.487370918990518	25.68085034480852	23.936860519874603
14	22.70381651247301	20.74012955778312	26.36167336692239	30.194380562821483
15	24.008779733755144	29.50892917883331	22.97754285957561	23.504748227835933
16	23.83577119021955	26.20601061047073	26.315643948836602	23.642574250473118
17	22.480493934225784	26.92864058367661	26.711306715493638	23.879558766603964
18	23.011688309408402	25.610090076172387	28.923738082155225	22.454483532263982
19	25.172513222392944	24.81763389496959	26.466378848384544	23.54347403425292
20	25.392625349570856	25.210488617990368	26.54087674917095	22.856009283267824
21	25.65048746511993	24.560043747657474	25.51706764297207	24.272401144250527
22	25.091561667907968	25.748918486086797	25.97744412050108	23.182075725504152
23	23.370151269975235	25.762285775478617	26.768513971197834	24.09904898334832
24	24.41936963329186	25.335668187393985	26.921218425158493	23.323743754155668
25	24.09690692239327	25.501179517001503	27.065164199738863	23.336749360866367
26	22.769686824361987	26.61900753247232	27.60544451769266	23.00586112547303
27	23.525652645453064	26.134786060803698	26.80486714030709	23.534694153436146
28	22.99148221412398	26.385868384473554	26.549052430884885	24.073596970517585
29	23.57185806140139	25.45432201716079	26.366389431411708	24.607430490026115
30	23.140104484640897	25.974128574130944	26.880019100947543	24.005747840280613
31	24.277916621677733	25.36067958309961	26.235922852535488	24.12548094268717
32	23.51912866660025	26.469218618536637	26.156504519102768	23.855148195760343
33	23.023917128136347	26.0206135070569	27.151973153195936	23.803496211610824
34	23.87988299037282	26.03257923715516	27.1689244199443	22.91861335252771
35	23.930420905855446	26.210223385004582	26.942346154808348	22.91700955433162
36	22.97303525146543	26.427604247820234	26.889518488200864	23.70984201251347
37	23.751221237819443	26.30276339894295	25.86733121900821	24.078684144229392
38	23.62823010146888	26.717768334230186	26.125010643742314	23.528990920558616
39	24.49130608624436	25.555765738996932	26.05799687767865	23.894931297080056
40	25.139425252396247	25.766866318975833	26.400934665987265	22.69277376264066
41	23.87366964838646	25.519445550221004	27.479427801227068	23.127457000165464
42	24.320651875753523	26.92639054874958	26.40538325921199	22.347574316284913
43	23.076993974079326	26.396385325368993	27.014513998305894	23.512106702245784
44	22.90743813767645	26.751875257572237	26.160079034903426	24.180607569847886
45	23.13219942049136	26.83203115056428	26.185328653024246	23.85044077592011
46	23.40425158394236	25.76980739892877	26.01865619892455	24.80728481820432
47	24.302130683252056	25.884990416818997	26.201386807205356	23.611492092723584
48	23.72290564777375	26.313983093062767	25.586214978189343	24.376896280974144
49	23.907068930255175	26.29433497212647	25.83061005764303	23.967986039975315
50	23.409200730482823	27.3794565091927	25.30466409157281	23.90667866875167
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	253.0
1	177.0
2	101.0
3	256.0
4	411.0
5	473.5
6	536.0
7	508.0
8	480.0
9	697.5
10	915.0
11	1361.5
12	1808.0
13	3274.5
14	4741.0
15	6586.5
16	8432.0
17	9146.5
18	9861.0
19	9962.0
20	10063.0
21	10886.0
22	11709.0
23	13346.5
24	14984.0
25	16654.5
26	18325.0
27	24179.0
28	30033.0
29	37008.5
30	43984.0
31	51454.5
32	58925.0
33	70882.5
34	82840.0
35	97194.5
36	111549.0
37	116914.0
38	122279.0
39	120734.5
40	119190.0
41	121506.0
42	123822.0
43	123606.5
44	123391.0
45	131956.0
46	140521.0
47	146629.0
48	152737.0
49	155498.0
50	158259.0
51	153696.0
52	149133.0
53	146750.0
54	144367.0
55	142575.5
56	140784.0
57	134525.5
58	128267.0
59	116786.0
60	105305.0
61	92332.5
62	79360.0
63	67899.5
64	56439.0
65	48434.5
66	40430.0
67	34775.5
68	29121.0
69	25361.0
70	21601.0
71	16796.0
72	11991.0
73	10147.0
74	8303.0
75	6530.5
76	4758.0
77	4073.5
78	3389.0
79	2756.5
80	2124.0
81	1729.0
82	1334.0
83	1074.5
84	815.0
85	615.0
86	415.0
87	288.0
88	161.0
89	118.5
90	76.0
91	67.5
92	59.0
93	50.0
94	41.0
95	44.5
96	48.0
97	37.5
98	27.0
99	26.0
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00926038897144728
2	8.777619878149081E-4
3	3.9499289451670863E-4
4	0.0
5	0.0
6	2.1944049695372703E-4
7	4.388809939074541E-5
8	0.0
9	9.216500872056536E-4
10	0.0011410905841593804
11	0.0
12	1.7555239756298163E-4
13	4.8276909329819943E-4
14	8.777619878149081E-5
15	0.004081593243339322
16	7.899857890334173E-4
17	0.0
18	2.6332859634447246E-4
19	4.388809939074541E-5
20	6.144333914704357E-4
21	0.0
22	1.3166429817223623E-4
23	5.705452920796902E-4
24	3.0721669573521783E-4
25	0.002062740671365034
26	0.005266571926889448
27	0.006188222014095102
28	0.004959355231154231
29	0.003159943156133669
30	0.004432698038465285
31	0.008075410287897154
32	0.0036427122494318686
33	0.004959355231154231
34	0.0033793836530873964
35	0.0034671598518688867
36	0.007768193592161937
37	0.004213257541511559
38	0.008250962685460135
39	0.003906040845776341
40	0.00267717406283547
41	0.0036427122494318686
42	0.0022382930689280158
43	0.001404419180503853
44	0.0015360834786760892
45	0.0017555239756298162
46	0.001316642981722362
47	0.0022382930689280158
48	0.0021505168701465246
49	7.022095902519265E-4
50	0.0012727548823316167
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2278522.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.853235082126865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.20809316212745	33.11831151673022
2	15.818691773348498	15.13951152243504
3	6.27612981012764	9.009993456299464
4	3.09258669635338	5.919611127698256
5	1.6735683841641444	4.0042830656711
6	1.0190571721479624	2.925910945255432
7	0.6364327486010546	2.131875615293929
8	0.4340836360965092	1.6617845026744529
9	0.3175796898727458	1.3677494001110504
>10	1.3639412614298108	11.250185033962708
>50	0.08266190053223837	2.7512297199276476
>100	0.0674853074967707	6.629330313897906
>500	0.006828055904080187	2.239392441786289
>1k	0.0028604017976551583	1.8508313382564918
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2343	0.1028298168725165	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.777619878149081E-5
2	0.0	0.0	0.0	8.777619878149081E-5	8.777619878149081E-5
3	0.0	0.0	0.0	8.777619878149081E-5	8.777619878149081E-5
4	0.0	0.0	0.0	8.777619878149081E-5	8.777619878149081E-5
5	0.0	0.0	0.0	8.777619878149081E-5	8.777619878149081E-5
6	0.0	0.0	0.0	8.777619878149081E-5	2.1944049695372703E-4
7	0.0	0.0	0.0	1.316642981722362E-4	2.1944049695372703E-4
8	0.0	0.0	0.0	1.316642981722362E-4	3.0721669573521783E-4
9	0.0	0.0	0.0	1.316642981722362E-4	3.9499289451670863E-4
10	0.0	0.0	0.0	2.1944049695372703E-4	4.3888099390745406E-4
11	0.0	0.0	0.0	2.1944049695372703E-4	4.3888099390745406E-4
12	0.0	0.0	0.0	3.0721669573521783E-4	5.266571926889448E-4
13	0.0	0.0	0.0	3.0721669573521783E-4	6.58321490861181E-4
14	0.0	0.0	0.0	3.0721669573521783E-4	7.022095902519265E-4
15	0.0	0.0	0.0	3.5110479512596326E-4	7.460976896426719E-4
16	0.0	0.0	0.0	3.9499289451670863E-4	7.899857890334173E-4
17	0.0	0.0	0.0	6.144333914704357E-4	7.899857890334173E-4
18	0.0	0.0	0.0	6.58321490861181E-4	7.899857890334173E-4
19	0.0	0.0	0.0	7.022095902519265E-4	7.899857890334173E-4
20	0.0	0.0	0.0	9.216500872056535E-4	8.338738884241626E-4
21	0.0	0.0	0.0	0.0010094262859871442	9.655381865963989E-4
22	0.0	0.0	0.0	0.001184978683550126	0.0010094262859871442
23	0.0	0.0	0.0	0.002062740671365034	0.0012727548823316167
24	0.0	0.0	0.0	0.0037743765476041047	0.001316642981722362
25	0.0	0.0	0.0	0.004696026634809758	0.0013605310811131074
26	0.0	0.0	0.0	0.005573788622624667	0.0014483072798945984
27	0.0	0.0	0.0	0.006670991107393301	0.0014483072798945984
28	0.0	0.0	0.0	0.010006486661089952	0.0014483072798945984
29	0.0	0.0	0.0	0.014746401395290456	0.0014921953792853438
30	0.0	0.0	0.0	0.0248406642551619	0.0014921953792853438
31	0.0	0.0	0.0	0.05319237646158343	0.0015360834786760892
32	0.0	0.0	0.0	0.08277295545094583	0.00162385967745758
33	0.0	0.0	0.0	0.1094130317811283	0.00162385967745758
34	0.0	0.0	0.0	0.140836910944902	0.0017994120750205616
35	0.0	0.0	0.0	0.17348965689161658	0.0017994120750205616
36	0.0	0.0	0.0	0.22224933531473473	0.0017994120750205616
37	0.0	0.0	0.0	0.29870240445341323	0.0017994120750205616
38	0.0	0.0	0.0	0.3978456209771071	0.001843300174411307
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	1730	0.0	20.344873	4
TGTAGGA	2890	0.0	19.02932	2
GTCCTAC	2440	0.0	18.935106	1
GATATAC	1315	0.0	18.905622	1
CTGTAGG	2950	0.0	18.793896	1
GTATCGA	95	1.59944E-5	18.524542	9
GTATCAA	6395	0.0	18.405666	1
AGGACCT	2130	0.0	18.383215	5
GTAGGAC	2860	0.0	18.382854	3
TTAGGAC	1575	0.0	18.29666	3
CGTCGTA	400	0.0	18.148262	10
TCCTACA	2655	0.0	18.062302	2
ATACACT	1385	0.0	17.94774	4
TAGGACG	2805	0.0	17.802217	4
TAGGACA	870	0.0	17.699453	4
ACTGTTC	1395	0.0	17.503315	8
CCTACAG	2670	0.0	17.466492	3
AGGACGT	2855	0.0	17.336344	5
GTCCTAA	1435	0.0	17.324663	1
GGACGTG	2845	0.0	17.165316	6
>>END_MODULE