FastQCFastQC Report
Thu 2 Feb 2017
SRR4064061_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064061_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2447239
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61740.25228430897023135No Hit
TATCAACGCAGAGTACTTTTTTTTT42190.17239836403391742No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG26090.10660993879224712No Hit
GTACTTTTTTTTTTTTTTTTTTTTT25300.10338181109405334No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG400.005284512414.2482665
CGTTATT2100.012.661762
GCGTTAT2350.012.5280841
CGCGCTA1405.2750693E-1112.21654816
ACGCGCG1405.2750693E-1112.21454913
CGGTTCT4650.012.05426912
GGTTCTA4650.012.05402313
GTATCAA41700.011.3646321
CGGTCCA4850.011.36083710
AACCGCG2100.011.30999857
GTATTAA3150.011.1553191
ACGGTAT3900.010.9622229
CGCATCG4200.010.85737613
TAATATA4550.010.8553784
GTATTAG3850.010.8538251
CCGTCGT4650.010.8286329
GGTTAAA1855.456968E-1210.7804881
CGAACGA1951.8189894E-1210.719933516
GTCTAGC1605.475158E-1010.6842341
CGACCAT4200.010.63096410
Produced by FastQC (version 0.11.5)