##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4064061_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2447239 Sequences flagged as poor quality 0 Sequence length 25 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.227984679878016 32.0 32.0 32.0 32.0 32.0 2 30.81999796505368 32.0 32.0 32.0 32.0 32.0 3 30.836267320028817 32.0 32.0 32.0 32.0 32.0 4 30.85953721724768 32.0 32.0 32.0 32.0 32.0 5 30.763006800725226 32.0 32.0 32.0 32.0 32.0 6 34.413020959538485 36.0 36.0 36.0 32.0 36.0 7 34.3436705609873 36.0 36.0 36.0 32.0 36.0 8 34.306222645193216 36.0 36.0 36.0 32.0 36.0 9 34.433709989093835 36.0 36.0 36.0 32.0 36.0 10 34.124859893128544 36.0 36.0 36.0 32.0 36.0 11 34.43543560722921 36.0 36.0 36.0 32.0 36.0 12 34.225089580543624 36.0 36.0 36.0 32.0 36.0 13 34.320832987705735 36.0 36.0 36.0 32.0 36.0 14 34.21439181052607 36.0 36.0 36.0 32.0 36.0 15 34.151162187264916 36.0 36.0 36.0 32.0 36.0 16 34.147458830134696 36.0 36.0 36.0 32.0 36.0 17 34.07228227402391 36.0 36.0 36.0 32.0 36.0 18 34.07158271014805 36.0 36.0 36.0 32.0 36.0 19 34.08094428047281 36.0 36.0 36.0 32.0 36.0 20 34.069766377538116 36.0 36.0 36.0 32.0 36.0 21 34.054027824826264 36.0 36.0 36.0 32.0 36.0 22 34.0300587723553 36.0 36.0 36.0 32.0 36.0 23 33.97924640789069 36.0 36.0 36.0 32.0 36.0 24 33.97012306521758 36.0 36.0 36.0 32.0 36.0 25 33.558784001072226 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 6.0 4 9.0 5 28.0 6 95.0 7 39.0 8 111.0 9 172.0 10 211.0 11 71.0 12 156.0 13 129.0 14 208.0 15 416.0 16 635.0 17 864.0 18 1109.0 19 1463.0 20 2410.0 21 3540.0 22 5738.0 23 9096.0 24 13332.0 25 19733.0 26 28478.0 27 37279.0 28 50762.0 29 68811.0 30 90546.0 31 123504.0 32 173267.0 33 243647.0 34 513400.0 35 1057974.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.73984212417423 16.946777509214737 11.259740519867737 26.053639846743295 2 16.77963642901948 19.462509935168555 37.272093873806504 26.48575976200546 3 18.87873854613094 23.656305471377994 28.41331836116914 29.05163762132193 4 12.576154759302128 15.268750855585363 35.544703952140345 36.61039043297217 5 14.723817572399655 36.51261400499051 33.36534477136069 15.39822365124914 6 34.469583810290175 35.249211565316145 16.862796222379245 13.418408402014437 7 30.673875246555177 30.462489690253946 20.44006778070506 18.423567282485813 8 28.63108396227649 32.41933558750049 19.053421472577206 19.89615897764581 9 27.552351151516834 13.956873002414477 18.304812847341744 40.18596299872694 10 15.782919087941917 26.45244828695756 31.38757725832256 26.377055366777967 11 37.170886270020546 20.991976450627064 22.403671412655743 19.433465866696647 12 24.957752329908406 23.447548899148178 28.671826477278255 22.922872293665158 13 29.481841536169934 19.396668219039064 25.253637069507985 25.867853175283017 14 23.930981242645117 19.360301988752294 25.145543891327225 31.563172877275363 15 25.34386907032588 26.952727790132435 22.21336339338393 25.490039746157755 16 25.966289451776305 25.629363881251727 23.49798253547941 24.906364131492566 17 24.11036422812096 25.736020476587672 25.042032612073335 25.11158268321803 18 24.891592022874043 24.820069747508782 25.90378147961554 24.384556750001636 19 25.58746821705553 24.791023817682646 25.404110580520744 24.21739738474108 20 26.00233339017547 24.026476167809438 24.803823063104176 25.167367378910914 21 27.021201598380028 23.946953476541992 24.41393822715905 24.617906697918933 22 26.19200774027548 23.90685170592351 24.777060192010193 25.124080361790813 23 24.63472306512006 23.96911095225554 25.43819327779205 25.957972704832354 24 24.910076355356654 24.708105968410997 25.182219081642 25.19959859459035 25 25.01309589383923 24.337997965996067 25.12968104086696 25.51922509929775 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 166.0 1 166.0 2 503.5 3 841.0 4 841.0 5 841.0 6 2186.5 7 3532.0 8 3532.0 9 3532.0 10 3744.5 11 3957.0 12 3957.0 13 3957.0 14 4688.5 15 5420.0 16 5420.0 17 5420.0 18 9673.5 19 13927.0 20 13927.0 21 13927.0 22 22747.0 23 31567.0 24 31567.0 25 31567.0 26 47606.0 27 63645.0 28 63645.0 29 63645.0 30 80056.0 31 96467.0 32 96467.0 33 96467.0 34 121903.0 35 147339.0 36 147339.0 37 147339.0 38 171533.0 39 195727.0 40 195727.0 41 195727.0 42 226994.5 43 258262.0 44 258262.0 45 258262.0 46 289727.0 47 321192.0 48 321192.0 49 321192.0 50 330776.0 51 340360.0 52 340360.0 53 340360.0 54 316862.0 55 293364.0 56 293364.0 57 293364.0 58 269294.5 59 245225.0 60 245225.0 61 245225.0 62 214209.5 63 183194.0 64 183194.0 65 183194.0 66 150209.0 67 117224.0 68 117224.0 69 117224.0 70 88548.5 71 59873.0 72 59873.0 73 59873.0 74 45826.0 75 31779.0 76 31779.0 77 31779.0 78 25457.0 79 19135.0 80 19135.0 81 19135.0 82 13265.5 83 7396.0 84 7396.0 85 7396.0 86 5669.0 87 3942.0 88 3942.0 89 3942.0 90 2842.5 91 1743.0 92 1743.0 93 1743.0 94 1206.0 95 669.0 96 669.0 97 669.0 98 981.0 99 1293.0 100 1293.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03620406507088192 2 0.005066934614886409 3 0.0012667336537216022 4 0.0034324395778262766 5 0.00804988805752115 6 0.012340437529803995 7 0.021452747361414233 8 0.032975937372688155 9 0.04744121845067033 10 0.056635253034133574 11 0.05369316196742534 12 0.06300978367866808 13 0.06211081140828501 14 0.06476686584350771 15 0.059985967860106834 16 0.06517548960277275 17 0.062069949032358504 18 0.07547280833625158 19 0.07130484599174826 20 0.07641264298256117 21 0.07204036875842532 22 0.0755136707121781 23 0.080539742951138 24 0.07481901032142754 25 0.07420607468252999 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2447239.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.13233158244035 #Duplication Level Percentage of deduplicated Percentage of total 1 75.3462400822997 37.772826912960056 2 14.004789179828114 14.041854698106315 3 4.628733478777715 6.96147604694481 4 1.981640674272276 3.9737706943947364 5 1.0630181730499533 2.6645789764750107 6 0.6685265852435235 2.0108877865862937 7 0.43263064802280354 1.5182148169583658 8 0.29760483537672083 1.1935699430114675 9 0.22247929144866394 1.0038065048217724 >10 1.1897030056698465 10.58257433201374 >50 0.08215000356057375 2.863201807776392 >100 0.0662573470325495 7.181990342890061 >500 0.010899749952310753 3.7957155797276854 >1k 0.005244992458254266 4.1818730557127415 >5k 8.19530071602229E-5 0.253658501620758 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6174 0.25228430897023135 No Hit TATCAACGCAGAGTACTTTTTTTTT 4219 0.17239836403391742 No Hit GTTCAAAGCAGGCCCGAGCCGCCTG 2609 0.10660993879224712 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2530 0.10338181109405334 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.172475185300659E-5 2 0.0 0.0 0.0 0.0 8.172475185300659E-5 3 0.0 0.0 0.0 0.0 8.172475185300659E-5 4 0.0 0.0 0.0 4.0862375926503295E-5 8.172475185300659E-5 5 0.0 0.0 0.0 4.0862375926503295E-5 8.172475185300659E-5 6 0.0 0.0 0.0 4.0862375926503295E-5 2.860366314855231E-4 7 0.0 0.0 0.0 4.0862375926503295E-5 2.860366314855231E-4 8 0.0 0.0 0.0 8.172475185300659E-5 2.860366314855231E-4 9 0.0 0.0 0.0 8.172475185300659E-5 3.2689900741202636E-4 10 0.0 0.0 0.0 8.172475185300659E-5 3.677613833385297E-4 11 0.0 0.0 0.0 8.172475185300659E-5 3.677613833385297E-4 12 0.0 0.0 0.0 8.172475185300659E-5 8.581098944565693E-4 13 0.0 0.0 0.0 8.172475185300659E-5 9.80697022236079E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGACGG 40 0.0052845124 14.248266 5 CGTTATT 210 0.0 12.66176 2 GCGTTAT 235 0.0 12.528084 1 CGCGCTA 140 5.2750693E-11 12.216548 16 ACGCGCG 140 5.2750693E-11 12.214549 13 CGGTTCT 465 0.0 12.054269 12 GGTTCTA 465 0.0 12.054023 13 GTATCAA 4170 0.0 11.364632 1 CGGTCCA 485 0.0 11.360837 10 AACCGCG 210 0.0 11.3099985 7 GTATTAA 315 0.0 11.155319 1 ACGGTAT 390 0.0 10.962222 9 CGCATCG 420 0.0 10.857376 13 TAATATA 455 0.0 10.855378 4 GTATTAG 385 0.0 10.853825 1 CCGTCGT 465 0.0 10.828632 9 GGTTAAA 185 5.456968E-12 10.780488 1 CGAACGA 195 1.8189894E-12 10.7199335 16 GTCTAGC 160 5.475158E-10 10.684234 1 CGACCAT 420 0.0 10.630964 10 >>END_MODULE