FastQCFastQC Report
Thu 2 Feb 2017
SRR4064061_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064061_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2447239
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA25520.1042807833644364No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT25280.10330008634220032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49650.021.5352441
TAACGGC1601.8189894E-1220.62758636
ACCGCGT550.004487482419.9980138
AACGCAG62300.017.1604126
TAGGACG1954.9112714E-1116.9213964
GTAAACG5750.016.45289227
TAAACGC5900.016.40716228
CGCAATA6950.016.1459136
TTAACGG2051.05501385E-1016.09957935
GGTATCA26150.016.0692061
TCTAGAT5850.015.7936252
CTAGCGG7200.015.58433229
TAATACT4700.015.4452734
TACGACG6600.015.3318095
TATCAAC71400.015.3125162
CAATACG7500.015.25438
TCAACGC70600.015.2364464
GCATTCG9050.015.07217422
TCTAGCG7650.014.95459528
ATCAACG72150.014.9399163
Produced by FastQC (version 0.11.5)