##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064061_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2447239
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32696520446103	32.0	32.0	32.0	32.0	32.0
2	31.414049465540554	32.0	32.0	32.0	32.0	32.0
3	31.478758715434005	32.0	32.0	32.0	32.0	32.0
4	31.564457333345864	32.0	32.0	32.0	32.0	32.0
5	31.51241092512828	32.0	32.0	32.0	32.0	32.0
6	35.09387722245355	36.0	36.0	36.0	36.0	36.0
7	35.1143415089413	36.0	36.0	36.0	36.0	36.0
8	35.0632140955583	36.0	36.0	36.0	36.0	36.0
9	35.168202615273785	36.0	36.0	36.0	36.0	36.0
10	35.02617235178093	36.0	36.0	36.0	36.0	36.0
11	35.17008187594264	36.0	36.0	36.0	36.0	36.0
12	35.073274821135165	36.0	36.0	36.0	36.0	36.0
13	35.11694280779278	36.0	36.0	36.0	36.0	36.0
14	35.05827996366518	36.0	36.0	36.0	36.0	36.0
15	35.03832032752012	36.0	36.0	36.0	36.0	36.0
16	35.04724181005615	36.0	36.0	36.0	36.0	36.0
17	35.0113854838044	36.0	36.0	36.0	36.0	36.0
18	35.00275248964241	36.0	36.0	36.0	36.0	36.0
19	35.00648036419818	36.0	36.0	36.0	36.0	36.0
20	34.99481905935628	36.0	36.0	36.0	36.0	36.0
21	34.99221612600976	36.0	36.0	36.0	36.0	36.0
22	34.98034111094176	36.0	36.0	36.0	36.0	36.0
23	34.92898650274861	36.0	36.0	36.0	32.0	36.0
24	34.9025371040589	36.0	36.0	36.0	32.0	36.0
25	34.87224255579451	36.0	36.0	36.0	32.0	36.0
26	34.81681928083036	36.0	36.0	36.0	32.0	36.0
27	34.81736724529153	36.0	36.0	36.0	32.0	36.0
28	34.793428839602505	36.0	36.0	36.0	32.0	36.0
29	34.766157698532915	36.0	36.0	36.0	32.0	36.0
30	34.750810198758685	36.0	36.0	36.0	32.0	36.0
31	34.74842751361841	36.0	36.0	36.0	32.0	36.0
32	34.70588038193245	36.0	36.0	36.0	32.0	36.0
33	34.67635568083052	36.0	36.0	36.0	32.0	36.0
34	34.67362157925728	36.0	36.0	36.0	32.0	36.0
35	34.635319639806326	36.0	36.0	36.0	32.0	36.0
36	34.61049043432211	36.0	36.0	36.0	32.0	36.0
37	34.60137036063907	36.0	36.0	36.0	32.0	36.0
38	34.5665646060724	36.0	36.0	36.0	32.0	36.0
39	34.56506577412341	36.0	36.0	36.0	32.0	36.0
40	34.5455139444901	36.0	36.0	36.0	32.0	36.0
41	34.523342427936136	36.0	36.0	36.0	32.0	36.0
42	34.46560429937575	36.0	36.0	36.0	32.0	36.0
43	34.45127345551456	36.0	36.0	36.0	32.0	36.0
44	34.38734140801123	36.0	36.0	36.0	32.0	36.0
45	34.35631256285144	36.0	36.0	36.0	32.0	36.0
46	34.332258108014784	36.0	36.0	36.0	32.0	36.0
47	34.29875014250754	36.0	36.0	36.0	32.0	36.0
48	34.25077812179358	36.0	36.0	36.0	32.0	36.0
49	34.23676477859335	36.0	36.0	36.0	32.0	36.0
50	33.63254018099581	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	32.0
21	65.0
22	253.0
23	739.0
24	1899.0
25	4221.0
26	8857.0
27	16123.0
28	27241.0
29	41932.0
30	60954.0
31	87106.0
32	126340.0
33	203570.0
34	425298.0
35	1442603.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.39631304390469	17.689992460170696	11.776232578192525	26.137461917732086
2	16.217301833254876	20.328354647209583	36.724348161514726	26.72999535802082
3	17.91508596665288	24.28940008859003	28.874751351324846	28.920762593432244
4	12.254463090854632	15.920798908484215	35.76720540985167	36.05753259080948
5	14.387029628082912	36.852959600594794	33.171709015752036	15.588301755570258
6	34.48833987814795	35.67041103615108	16.5843422972095	13.256906788491479
7	30.572817660413627	30.53367145628783	20.46390295010371	18.429607933194838
8	28.612080797993166	32.64033467920379	18.911679652048697	19.835904870754348
9	27.70820206160965	13.79679096311152	18.05571560149623	40.439291373782595
10	15.810457898261813	26.64143514044401	31.40875521973453	26.13935174155965
11	37.548713099712046	20.929480879865743	22.315452079222407	19.20635394119981
12	25.02475240444632	23.492698091766346	28.605484470854275	22.877065032933057
13	29.65908705500071	19.410695306475954	25.138330262505704	25.79188737601763
14	23.89019240560537	19.431634021541125	25.144469697526915	31.533703875326598
15	25.390182675738	27.064720490142914	22.14657783854755	25.398518995571536
16	26.047819040824184	25.654516583266112	23.4246040210426	24.873060354867103
17	24.18656289802508	25.800422435242325	24.96065974757676	25.052354919155835
18	24.9879762164044	24.81226756912807	25.922419320105604	24.277336894361916
19	25.740702678163167	24.658543492109672	25.41784878211632	24.182905047610838
20	26.15073242171538	23.961476350346352	24.81881511459515	25.068976113343115
21	27.258392008299964	23.882751133011528	24.37273188274623	24.486124975942275
22	26.2847555364501	23.86692578893086	24.903115187909954	24.945203486709087
23	24.70439976070846	23.894829406707355	25.473516114585344	25.927254717998842
24	25.026049860454474	24.65898178757207	25.179978996661532	25.134989355311927
25	25.13139474159511	24.19988345893235	25.347110132943445	25.321611666529094
26	25.011115088569273	24.982673538406715	25.454329245269143	24.55188212775487
27	25.480829348386635	24.590397061696045	24.75270991358843	25.176063676328887
28	24.809165296361797	24.43113278172433	25.561470841111106	25.198231080802763
29	24.839539993535425	24.689571642169795	25.40026634706109	25.07062201723369
30	24.769763443865678	24.85202216286082	25.555859309394535	24.822355083878968
31	25.348315653628717	24.57068925920144	24.684826130273773	25.396168956896066
32	25.20806695410084	24.607618027197958	24.516451458808117	25.667863559893085
33	24.716791615348217	24.324782664483983	25.180843908271623	25.777581811896184
34	25.469145675335625	24.457647044881803	25.201733764145473	24.8714735156371
35	25.862292305925745	24.28156780344962	25.254201896150203	24.601937994474433
36	24.936822145758583	24.927668304005625	24.848798149616275	25.28671140061952
37	25.73417577720514	24.704903549657033	24.782912152261023	24.778008520876803
38	24.96333356626582	24.578954100449476	25.189645864978438	25.268066468306266
39	25.574658510781322	24.491438813084123	24.606674951137016	25.327227724997535
40	25.734434788009747	24.677589134958165	25.083074941524718	24.504901135507364
41	24.924575804024176	25.09460970864582	25.282623646440626	24.698190840889374
42	25.946153132622236	25.0027071766538	24.77734748992624	24.27379220079773
43	25.317915390373948	24.242642179281397	25.045889329484567	25.39355310086009
44	25.059557521328337	24.82990862262148	24.5984617610066	25.512072095043585
45	25.03764495606624	25.086639585453945	24.71119116609261	25.164524292387195
46	25.005679932919584	24.794950335320358	24.838877874086922	25.36049185767314
47	25.13402133471724	24.663521556400518	25.143665068231456	25.05879204065079
48	25.898314559450537	25.2239912324074	24.146389701838107	24.731304506303957
49	25.063622901302367	25.253715217846125	24.399239630733824	25.28342225011768
50	25.1428765234646	25.390586371953788	24.501576892618477	24.964960211963135
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20.0
1	31.5
2	43.0
3	117.0
4	191.0
5	237.5
6	284.0
7	286.0
8	288.0
9	430.0
10	572.0
11	877.0
12	1182.0
13	2283.5
14	3385.0
15	4627.0
16	5869.0
17	6372.5
18	6876.0
19	6833.5
20	6791.0
21	7247.0
22	7703.0
23	8856.5
24	10010.0
25	11278.5
26	12547.0
27	15175.0
28	17803.0
29	21461.5
30	25120.0
31	29588.5
32	34057.0
33	39537.0
34	45017.0
35	52845.0
36	60673.0
37	72391.5
38	84110.0
39	93381.5
40	102653.0
41	114258.0
42	125863.0
43	131043.0
44	136223.0
45	149141.5
46	162060.0
47	173616.0
48	185172.0
49	191309.5
50	197447.0
51	192251.0
52	187055.0
53	185080.0
54	183105.0
55	182318.0
56	181531.0
57	175825.5
58	170120.0
59	154407.0
60	138694.0
61	121925.5
62	105157.0
63	91557.5
64	77958.0
65	65919.5
66	53881.0
67	46455.5
68	39030.0
69	35021.0
70	31012.0
71	23849.5
72	16687.0
73	14255.5
74	11824.0
75	9273.5
76	6723.0
77	5779.5
78	4836.0
79	4034.5
80	3233.0
81	2596.0
82	1959.0
83	1621.0
84	1283.0
85	932.0
86	581.0
87	417.0
88	253.0
89	193.5
90	134.0
91	113.0
92	92.0
93	62.0
94	32.0
95	35.5
96	39.0
97	36.5
98	34.0
99	30.5
100	27.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009561795966801772
2	2.860366314855231E-4
3	4.494861351915362E-4
4	0.0
5	0.0
6	3.677613833385296E-4
7	1.2258712777950988E-4
8	0.0
9	0.0010215593981625823
10	0.0014710455333541185
11	8.172475185300659E-5
12	8.172475185300659E-5
13	6.537980148240527E-4
14	5.312108870445429E-4
15	0.0047400356074743825
16	5.312108870445429E-4
17	0.0
18	2.4517425555901977E-4
19	8.172475185300659E-5
20	6.129356388975494E-4
21	0.0
22	1.2258712777950988E-4
23	6.129356388975494E-4
24	3.677613833385296E-4
25	0.001348458405574609
26	0.004699173231547879
27	0.004617448479694872
28	0.0038819257130178132
29	0.0022882930518841847
30	0.00335071482597327
31	0.007232640538991084
32	0.002860366314855231
33	0.004127099968576833
34	0.003187265322267257
35	0.002860366314855231
36	0.00731436529084409
37	0.0029829534426347405
38	0.007273502914917587
39	0.0036776138333852967
40	0.002083981172251668
41	0.003268990074120264
42	0.001634495037060132
43	0.0019205316685456549
44	0.0010215593981625823
45	0.0013075960296481054
46	0.001103284150015589
47	0.002206568300031178
48	0.0018388069166926483
49	2.860366314855231E-4
50	8.581098944565692E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2447239.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.92615877466349
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.08979346532006	40.271404900768815
2	13.808585030321243	14.61670727536447
3	4.5009125348627945	7.146480343530641
4	1.9243142823540635	4.0738625296089515
5	0.974906339595448	2.5799023859927344
6	0.6057325159349073	1.923545718800837
7	0.3910488991054316	1.4487701285898005
8	0.2711491420580516	1.1480706035342578
9	0.20161117778867607	0.960345468575134
>10	1.0920641216392448	10.16472309491403
>50	0.06822229786150207	2.502138358439616
>100	0.058766906938034214	6.646094591689977
>500	0.008854425476877297	3.288472260429298
>1k	0.004038860743837916	3.2294823397613115
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2552	0.1042807833644364	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2528	0.10330008634220032	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0431187963251647E-4
2	0.0	0.0	0.0	0.0	2.0431187963251647E-4
3	0.0	0.0	0.0	0.0	2.0431187963251647E-4
4	0.0	0.0	0.0	0.0	2.0431187963251647E-4
5	0.0	0.0	0.0	0.0	2.4517425555901977E-4
6	0.0	0.0	0.0	0.0	2.4517425555901977E-4
7	0.0	4.0862375926503295E-5	0.0	0.0	2.4517425555901977E-4
8	0.0	4.0862375926503295E-5	0.0	4.0862375926503295E-5	2.4517425555901977E-4
9	0.0	4.0862375926503295E-5	0.0	4.0862375926503295E-5	2.4517425555901977E-4
10	0.0	4.0862375926503295E-5	0.0	2.860366314855231E-4	2.4517425555901977E-4
11	0.0	4.0862375926503295E-5	0.0	2.860366314855231E-4	2.4517425555901977E-4
12	0.0	4.0862375926503295E-5	0.0	2.860366314855231E-4	5.720732629710462E-4
13	0.0	4.0862375926503295E-5	0.0	2.860366314855231E-4	6.537980148240527E-4
14	0.0	4.0862375926503295E-5	0.0	2.860366314855231E-4	6.94660390750556E-4
15	0.0	4.0862375926503295E-5	0.0	2.860366314855231E-4	8.581098944565693E-4
16	0.0	4.0862375926503295E-5	0.0	2.860366314855231E-4	8.989722703830725E-4
17	0.0	4.0862375926503295E-5	0.0	3.2689900741202636E-4	8.989722703830725E-4
18	0.0	4.0862375926503295E-5	0.0	3.677613833385297E-4	8.989722703830725E-4
19	0.0	4.0862375926503295E-5	0.0	3.677613833385297E-4	8.989722703830725E-4
20	0.0	4.0862375926503295E-5	0.0	3.677613833385297E-4	0.0011850089018685956
21	0.0	4.0862375926503295E-5	0.0	4.0862375926503295E-4	0.0013075960296481054
22	0.0	4.0862375926503295E-5	0.0	4.903485111180395E-4	0.001389320781501112
23	0.0	4.0862375926503295E-5	0.0	8.581098944565693E-4	0.0014301831574276155
24	0.0	4.0862375926503295E-5	0.0	0.0011441465259420924	0.0014710455333541187
25	0.0	4.0862375926503295E-5	0.0	0.0012667336537216022	0.0014710455333541187
26	0.0	4.0862375926503295E-5	0.0	0.001511907909280622	0.0016344950370601318
27	0.0	4.0862375926503295E-5	0.0	0.0017570821648396418	0.0016344950370601318
28	0.0	4.0862375926503295E-5	0.0	0.0023291554278106878	0.0016344950370601318
29	0.0	4.0862375926503295E-5	0.0	0.0037593385852383032	0.0016344950370601318
30	0.0	4.0862375926503295E-5	0.0	0.0076821266741826195	0.0016344950370601318
31	0.0	4.0862375926503295E-5	0.0	0.01785685827988194	0.001675357412986635
32	0.0	4.0862375926503295E-5	0.0	0.02925746116337636	0.0017570821648396418
33	0.0	4.0862375926503295E-5	0.0	0.03930960564129617	0.0017570821648396418
34	0.0	4.0862375926503295E-5	0.0	0.0520178045544387	0.0018388069166926483
35	0.0	8.172475185300659E-5	0.0	0.06754550740650996	0.0018388069166926483
36	0.0	8.172475185300659E-5	0.0	0.09210379533833843	0.0018796692926191516
37	4.0862375926503295E-5	8.172475185300659E-5	0.0	0.1275723376425433	0.0018796692926191516
38	4.0862375926503295E-5	8.172475185300659E-5	0.0	0.17824168379140737	0.001961394044472158
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4965	0.0	21.535244	1
TAACGGC	160	1.8189894E-12	20.627586	36
ACCGCGT	55	0.0044874824	19.998013	8
AACGCAG	6230	0.0	17.160412	6
TAGGACG	195	4.9112714E-11	16.921396	4
GTAAACG	575	0.0	16.452892	27
TAAACGC	590	0.0	16.407162	28
CGCAATA	695	0.0	16.14591	36
TTAACGG	205	1.05501385E-10	16.099579	35
GGTATCA	2615	0.0	16.069206	1
TCTAGAT	585	0.0	15.793625	2
CTAGCGG	720	0.0	15.584332	29
TAATACT	470	0.0	15.445273	4
TACGACG	660	0.0	15.331809	5
TATCAAC	7140	0.0	15.312516	2
CAATACG	750	0.0	15.254	38
TCAACGC	7060	0.0	15.236446	4
GCATTCG	905	0.0	15.072174	22
TCTAGCG	765	0.0	14.954595	28
ATCAACG	7215	0.0	14.939916	3
>>END_MODULE