FastQCFastQC Report
Thu 2 Feb 2017
SRR4064060_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064060_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2318578
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT42440.1830432273574579No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG30410.13115797700142068No Hit
TATCAACGCAGAGTACTTTTTTTTT28760.12404154615458268No Hit
GATTAAGAGGGACGGCCGGGGGCAT27930.12046176578920356No Hit
GGGTAGGCACACGCTGAGCCAGTCA27380.11808962217359088No Hit
GCGCAAGACGGACCAGAGCGAAAGC27010.11649381646854236No Hit
GAATAGGACCGCGGTTCTATTTTGT26980.11636442681678166No Hit
GTCTTGCGCCGGTCCAAGAATTTCA26690.11511366018309498No Hit
GTATCTGATCGTCTTCGAACCTCCG26170.112870906219243No Hit
ATCAGATACCGTCGTAGTTCCGACC25660.11067128213931125No Hit
CTATTGGAGCTGGAATTACCGCGGC25520.11006746376442803No Hit
GAACTACGACGGTATCTGATCGTCT24230.1045037087387183No Hit
GAATAACGCCGCCGCATCGCCAGTC24150.10415866966735644No Hit
GTACATGGGGAATAATTGCAATCCC23670.10208843523918538No Hit
GTCCTATTCCATTATTCCTAGCTGC23650.10200217547134494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTAC1300.014.6088781
CGTGCGG551.9585203E-413.82008210
CGTCTTA1400.013.57446715
CGACCAT4350.012.88682810
GGTATCA12100.012.8703021
GCGTTAT2600.012.7827691
CGAACGA2550.012.66922916
CGTCGTA5250.012.48743110
TTGCGCG2700.012.31757318
TAGAACC2950.012.2336884
CCGACCA4750.012.2016769
ACGAACG2650.012.19140815
ACTCTAA4450.012.17021310
CCGTCGT5550.011.9836299
AACGAAC2700.011.96512414
GACCATA4700.011.92717111
CGTTATT2950.011.9081522
ATAACGA3050.011.83769212
CGATAAC3050.011.83769210
ATCGCCA5000.011.78238316
Produced by FastQC (version 0.11.5)