FastQCFastQC Report
Thu 2 Feb 2017
SRR4064060_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064060_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2318578
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA29470.12710376791291905No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA29320.12645681965411557No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT28860.12447284499378498No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC27600.11903847961983595No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC26790.11554495902229729No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT26700.11515679006701522No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG26480.11420793262077016No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA24690.1064876833990489No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT24430.10536630641712291No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC23210.1001044605788548No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA23200.10006133069493457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32600.025.1053261
GGTATCA15250.022.2173081
TACCGTC6950.018.9915397
CGTCGTA7350.018.55698610
ATACCGT7350.018.5565856
ACCGTCG7150.018.4603088
CCGTCGT7700.017.1428249
AACGCAG47950.016.9748866
GTATAGT1854.2200554E-1016.6493341
GTATTAC2251.8189894E-1216.6229061
ATTCGTG2800.016.498944
TCAACGC50100.016.290334
CAACGCA50650.016.1134385
ATCAACG51500.015.8047723
TATCAAC52300.015.7726472
CGCAATA8800.015.75166736
CGAATGC8700.015.67711543
ATACGAA8850.015.66064840
TACGAAT8950.015.4856741
TAACGGC1452.2851382E-615.1740236
Produced by FastQC (version 0.11.5)