##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064060_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2318578
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.34724042063713	32.0	32.0	32.0	32.0	32.0
2	31.42803045659883	32.0	32.0	32.0	32.0	32.0
3	31.492974141909393	32.0	32.0	32.0	32.0	32.0
4	31.574628500744854	32.0	32.0	32.0	32.0	32.0
5	31.52909412579607	32.0	32.0	32.0	32.0	32.0
6	35.126713011164604	36.0	36.0	36.0	36.0	36.0
7	35.133416689022326	36.0	36.0	36.0	36.0	36.0
8	35.0831225000841	36.0	36.0	36.0	36.0	36.0
9	35.18200120936194	36.0	36.0	36.0	36.0	36.0
10	35.0431052998864	36.0	36.0	36.0	36.0	36.0
11	35.18512165646357	36.0	36.0	36.0	36.0	36.0
12	35.086073878040764	36.0	36.0	36.0	36.0	36.0
13	35.12909033036628	36.0	36.0	36.0	36.0	36.0
14	35.06852864126201	36.0	36.0	36.0	36.0	36.0
15	35.043466728313646	36.0	36.0	36.0	36.0	36.0
16	35.05766163570947	36.0	36.0	36.0	36.0	36.0
17	35.01519034511671	36.0	36.0	36.0	36.0	36.0
18	35.0161870767341	36.0	36.0	36.0	36.0	36.0
19	35.015599647715106	36.0	36.0	36.0	36.0	36.0
20	35.00417928575187	36.0	36.0	36.0	36.0	36.0
21	35.00581606484664	36.0	36.0	36.0	36.0	36.0
22	34.98890440606268	36.0	36.0	36.0	36.0	36.0
23	34.93940251309208	36.0	36.0	36.0	36.0	36.0
24	34.90132227598123	36.0	36.0	36.0	32.0	36.0
25	34.874403190231256	36.0	36.0	36.0	32.0	36.0
26	34.81751401074279	36.0	36.0	36.0	32.0	36.0
27	34.80863356764362	36.0	36.0	36.0	32.0	36.0
28	34.79283767895667	36.0	36.0	36.0	32.0	36.0
29	34.75991879505455	36.0	36.0	36.0	32.0	36.0
30	34.74438125437229	36.0	36.0	36.0	32.0	36.0
31	34.74227953512886	36.0	36.0	36.0	32.0	36.0
32	34.71092712861073	36.0	36.0	36.0	32.0	36.0
33	34.68402055052709	36.0	36.0	36.0	32.0	36.0
34	34.674843805125384	36.0	36.0	36.0	32.0	36.0
35	34.6412805607575	36.0	36.0	36.0	32.0	36.0
36	34.614144100392565	36.0	36.0	36.0	32.0	36.0
37	34.61244564556379	36.0	36.0	36.0	32.0	36.0
38	34.56656752544016	36.0	36.0	36.0	32.0	36.0
39	34.581900630472646	36.0	36.0	36.0	32.0	36.0
40	34.55092862953069	36.0	36.0	36.0	32.0	36.0
41	34.545867337652645	36.0	36.0	36.0	32.0	36.0
42	34.47118406195521	36.0	36.0	36.0	32.0	36.0
43	34.46415820386461	36.0	36.0	36.0	32.0	36.0
44	34.399081247212735	36.0	36.0	36.0	32.0	36.0
45	34.379892761856624	36.0	36.0	36.0	32.0	36.0
46	34.363192008205026	36.0	36.0	36.0	32.0	36.0
47	34.33590804363709	36.0	36.0	36.0	32.0	36.0
48	34.30043328281386	36.0	36.0	36.0	32.0	36.0
49	34.28518773144574	36.0	36.0	36.0	32.0	36.0
50	33.6845355213411	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	6.0
20	26.0
21	80.0
22	281.0
23	707.0
24	1932.0
25	4204.0
26	8482.0
27	15301.0
28	25765.0
29	39593.0
30	57071.0
31	81087.0
32	116599.0
33	189605.0
34	396098.0
35	1381737.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.953201380804266	17.724217862721005	11.742659355173208	26.579921401301526
2	16.459160801339102	19.993711638514963	36.0947204935458	27.45240706660013
3	17.86119708371719	23.73973935636134	28.900899175698104	29.49816438422336
4	12.34847393531725	15.720109480897342	35.443922956225755	36.48749362755965
5	14.561770188451714	36.561116339411484	33.08415761729819	15.792955854838612
6	34.99816481948164	35.47552740414039	16.165934822841464	13.360372953536507
7	30.81195923189094	30.352582640127974	20.40354924593835	18.431908882042734
8	28.800152507269544	32.310493759537096	18.795830892900735	20.09352284029263
9	27.519107855818447	13.552182852292532	18.077513134292182	40.85119615759684
10	16.038208247629886	26.241709822100557	30.914055595786515	26.80602633448304
11	37.91608477264944	20.64575787400726	22.08344942460422	19.35470792873908
12	24.914063558031792	23.342019706940558	28.14760279378618	23.59631394124147
13	29.57111495447625	18.79999309919476	25.214852258072863	26.414039688256125
14	24.04103731044914	19.20362222798247	24.457414107729196	32.297926353839195
15	25.545673067591647	26.822335620908127	21.671259500350878	25.96073181114935
16	26.473112621627216	25.29587330066938	22.93785798081568	25.293156096887724
17	24.681119203235777	25.734566617987404	24.313393812931892	25.27092036584493
18	25.61597154801682	24.40043940820496	25.351714179368084	24.631874864410136
19	26.099704215256075	24.469351473187444	24.74529647050908	24.685647841047402
20	26.413429352577438	23.607035909742372	24.12321769992879	25.856317037751403
21	27.724363812647233	23.65618064175542	23.737739252248577	24.88171629334877
22	26.725651197675816	23.477275256256647	24.355401207811354	25.44167233825619
23	24.90345308859543	23.41399539887223	25.117249398549617	26.56530211398272
24	25.38728612895017	24.392319403770426	24.519251090111577	25.701143377167824
25	25.410399239174737	23.84941353052682	24.7127172977764	26.027469932522045
26	25.42007645635203	24.739793491073982	24.895759963458296	24.94437008911569
27	26.07513999842134	24.072050824290173	24.19489315779417	25.65791601949432
28	25.269184518849386	23.971310563281868	25.192410212880485	25.567094704988257
29	25.166959381124578	24.177538507190768	25.08910826179369	25.566393849890968
30	25.07621385772378	24.3777809216054	25.26250002156589	25.283505199104926
31	25.80711392073099	24.242964064624324	23.9977932904989	25.952128724145783
32	25.649128830291414	24.08936498379119	23.90903225027302	26.352473935644376
33	25.122958297048616	23.810109171181498	24.555126680581022	26.511805851188868
34	25.960036333742504	23.878437235012548	24.749040977250004	25.412485453994943
35	26.395625126967154	23.612203166426067	24.907602415858783	25.084569290747993
36	25.149122303796716	24.47706369783631	24.385405618273644	25.988408380093333
37	26.303864753469064	24.13228081011278	24.287295362675394	25.27655907374276
38	25.27157937234702	23.857749129457904	24.80608251527034	26.064588982924736
39	26.125673825673566	23.942344934909883	24.040987233866357	25.890994005550194
40	26.233476974622032	24.150541706365345	24.578575781742302	25.037405537270317
41	25.025382812493262	24.712637233243246	24.999115806871515	25.262864147391976
42	26.594491593592316	24.51554412301938	24.38753192217997	24.502432361208335
43	25.71642006053805	23.492004889279556	24.713850342154682	26.077724708027716
44	25.34828925184609	24.286805486367108	24.273995739573103	26.090909522213696
45	25.405944420291355	24.45707305964433	24.38815049444824	25.748832025616075
46	25.216449562960364	24.279054962705203	24.571090429638474	25.933405044695963
47	25.502764032256604	23.970458906558587	24.92627881509558	25.60049824608923
48	26.311265270491052	24.692002325617505	23.913058480309026	25.083673923582417
49	25.296853872977465	24.859341877411243	24.13751609292817	25.706288156683122
50	25.44399650987711	24.898912808604372	24.108545754812397	25.548544926706125
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	18.0
1	36.0
2	54.0
3	142.5
4	231.0
5	276.0
6	321.0
7	281.0
8	241.0
9	342.0
10	443.0
11	658.0
12	873.0
13	1731.5
14	2590.0
15	3412.0
16	4234.0
17	4318.5
18	4403.0
19	4347.5
20	4292.0
21	4941.0
22	5590.0
23	6412.5
24	7235.0
25	9052.5
26	10870.0
27	13352.0
28	15834.0
29	18799.0
30	21764.0
31	24921.5
32	28079.0
33	32354.0
34	36629.0
35	43379.5
36	50130.0
37	62078.0
38	74026.0
39	82695.0
40	91364.0
41	102033.5
42	112703.0
43	116424.5
44	120146.0
45	134063.5
46	147981.0
47	159923.5
48	171866.0
49	179452.5
50	187039.0
51	180945.5
52	174852.0
53	174249.5
54	173647.0
55	176265.0
56	178883.0
57	175512.0
58	172141.0
59	157748.5
60	143356.0
61	125997.5
62	108639.0
63	95279.5
64	81920.0
65	69853.0
66	57786.0
67	49984.5
68	42183.0
69	38314.0
70	34445.0
71	26363.5
72	18282.0
73	15590.0
74	12898.0
75	9950.0
76	7002.0
77	6108.0
78	5214.0
79	4365.0
80	3516.0
81	2821.0
82	2126.0
83	1776.0
84	1426.0
85	1029.5
86	633.0
87	453.5
88	274.0
89	204.5
90	135.0
91	112.0
92	89.0
93	73.5
94	58.0
95	64.0
96	70.0
97	45.5
98	21.0
99	23.5
100	26.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00944544457853046
2	3.8816895528207376E-4
3	4.312988392023042E-4
4	0.0
5	0.0
6	2.156494196011521E-4
7	4.312988392023041E-5
8	0.0
9	0.001250766633686682
10	9.057275623248388E-4
11	0.0
12	1.7251953568092165E-4
13	3.450390713618433E-4
14	2.156494196011521E-4
15	0.004787417115145577
16	7.763379105641475E-4
17	0.0
18	2.156494196011521E-4
19	0.0
20	6.469482588034563E-4
21	0.0
22	1.7251953568092165E-4
23	6.900781427236866E-4
24	3.450390713618433E-4
25	0.0018545850085699078
26	0.005650014793550185
27	0.006857651543316637
28	0.00405420908850166
29	0.002156494196011521
30	0.004399248159863502
31	0.008367197480524701
32	0.0037091700171398155
33	0.004701157347305115
34	0.00310535164225659
35	0.002803442454814977
36	0.007720249221721245
37	0.0037091700171398155
38	0.008410327364444931
39	0.004312988392023042
40	0.00306222175833636
41	0.0034503907136184337
42	0.0021133643120912903
43	0.001552675821128295
44	0.001337026401527143
45	0.0014664160532878343
46	0.00103511721408553
47	0.0019408447764103689
48	0.0023290137316924423
49	5.606884909629955E-4
50	0.0015095459372080646
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2318578.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.21518191593564
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.99624731393646	37.40169136483817
2	13.502959594094513	13.2910122565378
3	4.387479663206232	6.477918293314882
4	1.9435984314590566	3.826182015031385
5	1.0592718426785357	2.606612821792624
6	0.6502752665958573	1.92020493245692
7	0.43391907789052353	1.4948784448624013
8	0.3222062151637974	1.268594999498511
9	0.24107799685344025	1.0678227723964278
>10	1.2945605967662472	11.22416190385332
>50	0.08138994105925446	2.7831275185134015
>100	0.06837988623057194	7.320998876110409
>500	0.012452220584128811	4.311860746048887
>1k	0.006181953481482349	5.004933054744864
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2947	0.12710376791291905	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2932	0.12645681965411557	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2886	0.12447284499378498	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2760	0.11903847961983595	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	2679	0.11554495902229729	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2670	0.11515679006701522	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	2648	0.11420793262077016	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	2469	0.1064876833990489	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	2443	0.10536630641712291	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	2321	0.1001044605788548	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	2320	0.10006133069493457	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2938965176069125E-4
2	0.0	0.0	0.0	0.0	1.2938965176069125E-4
3	0.0	0.0	0.0	0.0	1.2938965176069125E-4
4	0.0	0.0	0.0	0.0	1.2938965176069125E-4
5	0.0	0.0	0.0	0.0	1.7251953568092168E-4
6	0.0	0.0	0.0	0.0	2.1564941960115208E-4
7	0.0	0.0	0.0	4.312988392023042E-5	2.1564941960115208E-4
8	0.0	0.0	0.0	4.312988392023042E-5	2.1564941960115208E-4
9	0.0	0.0	0.0	4.312988392023042E-5	2.1564941960115208E-4
10	0.0	0.0	0.0	4.312988392023042E-5	2.587793035213825E-4
11	0.0	0.0	0.0	8.625976784046084E-5	2.587793035213825E-4
12	0.0	0.0	0.0	8.625976784046084E-5	4.3129883920230415E-4
13	0.0	0.0	0.0	8.625976784046084E-5	5.17558607042765E-4
14	0.0	0.0	0.0	8.625976784046084E-5	5.17558607042765E-4
15	0.0	0.0	0.0	8.625976784046084E-5	6.038183748832258E-4
16	4.312988392023042E-5	0.0	0.0	2.1564941960115208E-4	6.038183748832258E-4
17	4.312988392023042E-5	0.0	0.0	2.1564941960115208E-4	6.038183748832258E-4
18	4.312988392023042E-5	0.0	0.0	2.1564941960115208E-4	6.900781427236867E-4
19	4.312988392023042E-5	0.0	0.0	2.1564941960115208E-4	6.900781427236867E-4
20	4.312988392023042E-5	0.0	0.0	2.587793035213825E-4	8.19467794484378E-4
21	4.312988392023042E-5	0.0	0.0	2.587793035213825E-4	8.625976784046083E-4
22	4.312988392023042E-5	0.0	0.0	3.019091874416129E-4	9.057275623248388E-4
23	4.312988392023042E-5	0.0	0.0	6.038183748832258E-4	9.057275623248388E-4
24	4.312988392023042E-5	0.0	0.0	7.763379105641475E-4	9.057275623248388E-4
25	4.312988392023042E-5	0.0	0.0	0.001121376981925991	9.057275623248388E-4
26	4.312988392023042E-5	0.0	0.0	0.0014232861693676037	9.488574462450692E-4
27	4.312988392023042E-5	0.0	0.0	0.0019408447764103687	9.488574462450692E-4
28	4.312988392023042E-5	0.0	0.0	0.003493520597538664	9.488574462450692E-4
29	4.312988392023042E-5	0.0	0.0	0.007073300962917788	9.488574462450692E-4
30	4.312988392023042E-5	0.0	0.0	0.014750420300718802	9.488574462450692E-4
31	4.312988392023042E-5	0.0	0.0	0.03484894620754618	9.488574462450692E-4
32	4.312988392023042E-5	0.0	0.0	0.05408487443596894	9.488574462450692E-4
33	4.312988392023042E-5	0.0	0.0	0.07461469918199862	9.488574462450692E-4
34	4.312988392023042E-5	0.0	0.0	0.09958690197181204	0.0010782470980057605
35	4.312988392023042E-5	0.0	0.0	0.12831140466268548	0.0010782470980057605
36	4.312988392023042E-5	0.0	0.0	0.17165693800251705	0.0010782470980057605
37	4.312988392023042E-5	0.0	0.0	0.23337580189236679	0.0010782470980057605
38	4.312988392023042E-5	0.0	0.0	0.31864358240266233	0.0010782470980057605
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3260	0.0	25.105326	1
GGTATCA	1525	0.0	22.217308	1
TACCGTC	695	0.0	18.991539	7
CGTCGTA	735	0.0	18.556986	10
ATACCGT	735	0.0	18.556585	6
ACCGTCG	715	0.0	18.460308	8
CCGTCGT	770	0.0	17.142824	9
AACGCAG	4795	0.0	16.974886	6
GTATAGT	185	4.2200554E-10	16.649334	1
GTATTAC	225	1.8189894E-12	16.622906	1
ATTCGTG	280	0.0	16.4989	44
TCAACGC	5010	0.0	16.29033	4
CAACGCA	5065	0.0	16.113438	5
ATCAACG	5150	0.0	15.804772	3
TATCAAC	5230	0.0	15.772647	2
CGCAATA	880	0.0	15.751667	36
CGAATGC	870	0.0	15.677115	43
ATACGAA	885	0.0	15.660648	40
TACGAAT	895	0.0	15.48567	41
TAACGGC	145	2.2851382E-6	15.17402	36
>>END_MODULE