FastQCFastQC Report
Thu 2 Feb 2017
SRR4064059_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064059_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1873403
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37220.19867588554091137No Hit
TATCAACGCAGAGTACTTTTTTTTT25450.1358490404894195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA1400.013.57322216
AACCGCG1750.012.4856987
CCGTCGT2400.012.2711439
CGTCGTA2400.012.27081510
ACCGTCG2650.011.8304888
CGGACAT1850.011.8095335
CGGTCCA3300.011.80301410
CGACCAT2100.011.761888510
CTTACAC1701.8189894E-1211.7324573
GAACCGC2450.011.6313946
GTCGTAG2550.011.54900411
CCCTTAA1406.82121E-1011.5344661
AATTCCG1657.2759576E-1211.5139055
GTATCAA25000.011.4367621
ACGAACG1751.8189894E-1211.40150715
TTCCGAT1751.8189894E-1211.3999847
AGGCCCG3200.011.28123510
TTTTCGG2200.011.22875616
ATACCGT3150.011.1575236
AACCGCT600.005876483411.0833177
Produced by FastQC (version 0.11.5)