##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064059_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1873403
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25408201011742	32.0	32.0	32.0	32.0	32.0
2	30.845093127319643	32.0	32.0	32.0	32.0	32.0
3	30.86596316969707	32.0	32.0	32.0	32.0	32.0
4	30.895326312597984	32.0	32.0	32.0	32.0	32.0
5	30.801406851595733	32.0	32.0	32.0	32.0	32.0
6	34.46068037683296	36.0	36.0	36.0	32.0	36.0
7	34.4033083111322	36.0	36.0	36.0	32.0	36.0
8	34.367068911494215	36.0	36.0	36.0	32.0	36.0
9	34.5015407789995	36.0	36.0	36.0	32.0	36.0
10	34.20008722095566	36.0	36.0	36.0	32.0	36.0
11	34.48085596105056	36.0	36.0	36.0	32.0	36.0
12	34.29197134839647	36.0	36.0	36.0	32.0	36.0
13	34.38222742250333	36.0	36.0	36.0	32.0	36.0
14	34.282016202600296	36.0	36.0	36.0	32.0	36.0
15	34.21328406114434	36.0	36.0	36.0	32.0	36.0
16	34.212768421957264	36.0	36.0	36.0	32.0	36.0
17	34.148100008380474	36.0	36.0	36.0	32.0	36.0
18	34.13656218122849	36.0	36.0	36.0	32.0	36.0
19	34.15196303197977	36.0	36.0	36.0	32.0	36.0
20	34.13088748123068	36.0	36.0	36.0	32.0	36.0
21	34.12180561256708	36.0	36.0	36.0	32.0	36.0
22	34.1015211356019	36.0	36.0	36.0	32.0	36.0
23	34.044056724580884	36.0	36.0	36.0	32.0	36.0
24	34.03203955582435	36.0	36.0	36.0	32.0	36.0
25	33.64128060006309	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	8.0
4	13.0
5	21.0
6	84.0
7	31.0
8	95.0
9	117.0
10	176.0
11	62.0
12	111.0
13	93.0
14	171.0
15	272.0
16	511.0
17	677.0
18	874.0
19	1196.0
20	1836.0
21	2687.0
22	4431.0
23	6597.0
24	9927.0
25	14630.0
26	21203.0
27	27654.0
28	37449.0
29	50903.0
30	66584.0
31	90362.0
32	126736.0
33	178214.0
34	381478.0
35	848198.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.2900112086012	17.0534675375093	11.320900810554347	26.335620443335156
2	16.61203095898879	19.587822029053278	37.78682411999677	26.01332289196116
3	18.698151510088522	23.86932192143878	28.59968174949169	28.832844818981012
4	12.657980539487928	15.435331270698413	35.84484757640397	36.06184061340969
5	14.63836624876217	36.44935098906437	33.752091969240375	15.160190792933081
6	34.14251982081588	35.47631084847666	16.938195783909197	13.442973546798267
7	30.150370640885182	30.327415465901254	21.030159553906838	18.492054339306726
8	27.925104592456528	33.21119533091799	19.42704425856163	19.43665581806386
9	27.55861484086862	14.174318736989166	18.331109898220433	39.93595652392178
10	15.820428707393905	26.640606390126504	31.890440019526512	25.648524882953076
11	37.10156878369328	20.94428238320108	22.743518232690988	19.210630600414653
12	24.93828268293803	23.40264046498163	29.03379149962131	22.625285352459027
13	29.630763182717956	19.575138367662188	25.110592644106944	25.68350580551291
14	23.549853751503612	19.62357837701559	25.236839237755827	31.589728633724967
15	24.959635272709598	27.36111622958464	22.212270129354064	25.466978368351707
16	25.633336680647044	25.654382025352696	23.90339866292622	24.808882631074038
17	24.06645209601627	25.93915608145424	25.24197950504896	24.75241231748053
18	24.7056680436614	25.010590554987814	26.169915772367975	24.11382562898281
19	25.60764867300459	24.88158456333857	25.24851616790713	24.262250595749705
20	25.89283472521156	24.43236422710025	24.870895348172557	24.80390569951563
21	26.5625	24.227564102564102	24.508867521367524	24.701068376068374
22	25.961961204766627	24.359498293473425	24.848885989921637	24.82965451183831
23	24.54062128046943	24.371753257160197	25.401362056033626	25.686263406336746
24	24.958733753919628	24.83741900330664	25.241961762616256	24.96188548015748
25	24.976495174031868	24.603195801610724	25.227889971772843	25.19241905258457
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	138.0
1	138.0
2	351.5
3	565.0
4	565.0
5	565.0
6	1528.0
7	2491.0
8	2491.0
9	2491.0
10	2833.5
11	3176.0
12	3176.0
13	3176.0
14	3890.0
15	4604.0
16	4604.0
17	4604.0
18	8070.5
19	11537.0
20	11537.0
21	11537.0
22	18784.0
23	26031.0
24	26031.0
25	26031.0
26	39145.5
27	52260.0
28	52260.0
29	52260.0
30	65952.0
31	79644.0
32	79644.0
33	79644.0
34	99558.0
35	119472.0
36	119472.0
37	119472.0
38	137119.5
39	154767.0
40	154767.0
41	154767.0
42	176788.0
43	198809.0
44	198809.0
45	198809.0
46	220895.5
47	242982.0
48	242982.0
49	242982.0
50	248917.0
51	254852.0
52	254852.0
53	254852.0
54	238941.0
55	223030.0
56	223030.0
57	223030.0
58	204238.5
59	185447.0
60	185447.0
61	185447.0
62	161256.0
63	137065.0
64	137065.0
65	137065.0
66	111859.0
67	86653.0
68	86653.0
69	86653.0
70	65353.5
71	44054.0
72	44054.0
73	44054.0
74	33274.0
75	22494.0
76	22494.0
77	22494.0
78	17826.5
79	13159.0
80	13159.0
81	13159.0
82	9039.0
83	4919.0
84	4919.0
85	4919.0
86	3779.0
87	2639.0
88	2639.0
89	2639.0
90	1893.0
91	1147.0
92	1147.0
93	1147.0
94	808.5
95	470.0
96	470.0
97	470.0
98	734.0
99	998.0
100	998.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.039180037610700956
2	0.00459057661378785
3	0.0019216367220507282
4	0.003789894646266713
5	0.00896763803623673
6	0.01313118426734664
7	0.023006261866773993
8	0.03512324897526053
9	0.049375387997136755
10	0.05748896526801762
11	0.05321866144123822
12	0.06175926909479701
13	0.06496199696488156
14	0.06656336089992383
15	0.05871667761821669
16	0.06699039128260177
17	0.06069169313810215
18	0.07750601445604603
19	0.07430328658596148
20	0.07857359041274088
21	0.07489045336214366
22	0.07857359041274088
23	0.08150942429365171
24	0.07542424134049107
25	0.07723912046687231
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1873403.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.571145751488636
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.61888215811722	43.13370213476353
2	13.41514337292153	14.909897753074786
3	3.994154677365684	6.658792551896356
4	1.6879945827236165	3.752151719370294
5	0.8844156538548885	2.457399560263407
6	0.5515105331807901	1.8388843333722542
7	0.37239646971654994	1.4486148947170754
8	0.2374279680413453	1.055531537400431
9	0.19975200373963473	0.9990402942570432
>10	0.9015400934428333	8.835826194730691
>50	0.06421799659327497	2.4956731665593415
>100	0.06185447539268931	7.258938628621146
>500	0.008008389527312441	2.995677924478938
>1k	0.0027016253827078112	2.1598693064947305
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3722	0.19867588554091137	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2545	0.1358490404894195	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6013639350422734E-4
2	0.0	0.0	0.0	0.0	1.6013639350422734E-4
3	0.0	0.0	0.0	0.0	1.6013639350422734E-4
4	0.0	0.0	0.0	5.337879783474244E-5	1.6013639350422734E-4
5	0.0	0.0	0.0	5.337879783474244E-5	2.1351519133896977E-4
6	5.337879783474244E-5	0.0	0.0	5.337879783474244E-5	2.668939891737122E-4
7	5.337879783474244E-5	0.0	0.0	5.337879783474244E-5	2.668939891737122E-4
8	5.337879783474244E-5	0.0	0.0	1.0675759566948488E-4	2.668939891737122E-4
9	5.337879783474244E-5	0.0	0.0	1.0675759566948488E-4	2.668939891737122E-4
10	5.337879783474244E-5	0.0	0.0	1.0675759566948488E-4	2.668939891737122E-4
11	5.337879783474244E-5	0.0	0.0	1.0675759566948488E-4	2.668939891737122E-4
12	5.337879783474244E-5	0.0	0.0	1.0675759566948488E-4	2.668939891737122E-4
13	5.337879783474244E-5	0.0	0.0	1.0675759566948488E-4	3.202727870084547E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	140	0.0	13.573222	16
AACCGCG	175	0.0	12.485698	7
CCGTCGT	240	0.0	12.271143	9
CGTCGTA	240	0.0	12.270815	10
ACCGTCG	265	0.0	11.830488	8
CGGACAT	185	0.0	11.809533	5
CGGTCCA	330	0.0	11.803014	10
CGACCAT	210	0.0	11.7618885	10
CTTACAC	170	1.8189894E-12	11.732457	3
GAACCGC	245	0.0	11.631394	6
GTCGTAG	255	0.0	11.549004	11
CCCTTAA	140	6.82121E-10	11.534466	1
AATTCCG	165	7.2759576E-12	11.513905	5
GTATCAA	2500	0.0	11.436762	1
ACGAACG	175	1.8189894E-12	11.401507	15
TTCCGAT	175	1.8189894E-12	11.399984	7
AGGCCCG	320	0.0	11.281235	10
TTTTCGG	220	0.0	11.228756	16
ATACCGT	315	0.0	11.157523	6
AACCGCT	60	0.0058764834	11.083317	7
>>END_MODULE