FastQCFastQC Report
Thu 2 Feb 2017
SRR4064059_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064059_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1873403
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28450.023.586281
TAGGACG4800.018.7909954
AACGCAG38250.017.6568856
TGTAGGA7100.017.0420992
GTGTATA3250.016.9238261
CGAGCCG5550.016.64805215
AGGACGT5500.016.3994125
TTAGGAC4050.016.2957133
CGTTTAT5300.016.18896331
TCAACGC41450.016.0814514
ATCAACG41900.015.9087383
TATCAAC43250.015.8703772
CAACGCA42300.015.8103095
ATAACGC5550.015.4589063
GGTATCA14550.015.4233631
ACGCAGA43950.015.3168587
TCGTTTA5500.015.20026730
CGCAGAG44600.015.1922828
CAATACG4850.014.96973638
GTAGGAC7350.014.965453
Produced by FastQC (version 0.11.5)