##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064059_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1873403
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3509244940891	32.0	32.0	32.0	32.0	32.0
2	31.418171637389285	32.0	32.0	32.0	32.0	32.0
3	31.490327494938356	32.0	32.0	32.0	32.0	32.0
4	31.57279186592527	32.0	32.0	32.0	32.0	32.0
5	31.526297331647275	32.0	32.0	32.0	32.0	32.0
6	35.118782237457715	36.0	36.0	36.0	36.0	36.0
7	35.138977571830516	36.0	36.0	36.0	36.0	36.0
8	35.085472266244906	36.0	36.0	36.0	36.0	36.0
9	35.18951608383247	36.0	36.0	36.0	36.0	36.0
10	35.04803451259553	36.0	36.0	36.0	36.0	36.0
11	35.1907528705783	36.0	36.0	36.0	36.0	36.0
12	35.09942228127103	36.0	36.0	36.0	36.0	36.0
13	35.13697319797182	36.0	36.0	36.0	36.0	36.0
14	35.07948102997593	36.0	36.0	36.0	36.0	36.0
15	35.06157404466631	36.0	36.0	36.0	36.0	36.0
16	35.06856239687883	36.0	36.0	36.0	36.0	36.0
17	35.03247619439063	36.0	36.0	36.0	36.0	36.0
18	35.03609687824777	36.0	36.0	36.0	36.0	36.0
19	35.0304776921997	36.0	36.0	36.0	36.0	36.0
20	35.025056007703625	36.0	36.0	36.0	36.0	36.0
21	35.02490654706969	36.0	36.0	36.0	36.0	36.0
22	35.00036831370506	36.0	36.0	36.0	36.0	36.0
23	34.95764125497824	36.0	36.0	36.0	36.0	36.0
24	34.9321443384045	36.0	36.0	36.0	36.0	36.0
25	34.90528893142586	36.0	36.0	36.0	32.0	36.0
26	34.84406345030941	36.0	36.0	36.0	32.0	36.0
27	34.840657349219576	36.0	36.0	36.0	32.0	36.0
28	34.82008516053406	36.0	36.0	36.0	32.0	36.0
29	34.79157287567064	36.0	36.0	36.0	32.0	36.0
30	34.7743368618498	36.0	36.0	36.0	32.0	36.0
31	34.77257803046115	36.0	36.0	36.0	32.0	36.0
32	34.73119611743976	36.0	36.0	36.0	32.0	36.0
33	34.712345394984425	36.0	36.0	36.0	32.0	36.0
34	34.699294278913825	36.0	36.0	36.0	32.0	36.0
35	34.6623385358089	36.0	36.0	36.0	32.0	36.0
36	34.64173271848075	36.0	36.0	36.0	32.0	36.0
37	34.631715119491105	36.0	36.0	36.0	32.0	36.0
38	34.58769789522062	36.0	36.0	36.0	32.0	36.0
39	34.58854074643843	36.0	36.0	36.0	32.0	36.0
40	34.570540348232605	36.0	36.0	36.0	32.0	36.0
41	34.547123603410476	36.0	36.0	36.0	32.0	36.0
42	34.49285231207594	36.0	36.0	36.0	32.0	36.0
43	34.47770981470618	36.0	36.0	36.0	32.0	36.0
44	34.426275072688576	36.0	36.0	36.0	32.0	36.0
45	34.39674218521055	36.0	36.0	36.0	32.0	36.0
46	34.38181320303213	36.0	36.0	36.0	32.0	36.0
47	34.3467187786077	36.0	36.0	36.0	32.0	36.0
48	34.306521874898245	36.0	36.0	36.0	32.0	36.0
49	34.28368162109274	36.0	36.0	36.0	32.0	36.0
50	33.696487621723676	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	13.0
21	59.0
22	181.0
23	511.0
24	1435.0
25	3164.0
26	6596.0
27	11870.0
28	20369.0
29	31234.0
30	45494.0
31	64891.0
32	94055.0
33	151986.0
34	319876.0
35	1121663.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.54726001912192	17.898211817687294	11.789661031697436	26.764867131493354
2	15.880971050922044	20.347359043190615	37.29607188667835	26.475598019208995
3	18.0786997744206	24.4594581610528	28.80749312212354	28.654348942403058
4	12.324150222883171	16.159363468511582	35.88080087413119	35.635685434474055
5	14.263508705815035	36.84850510007724	33.54425075651101	15.343735437596715
6	34.29897512544038	35.782854702679614	16.67513611615245	13.243034055727554
7	30.04710147635158	30.49014573487164	21.092910117529502	18.369842671247284
8	27.895439475649393	33.41299229263538	19.29686244764207	19.394705784073153
9	27.61239601660318	14.010641750508174	18.170991835061407	40.20597039782724
10	15.726814634510882	26.823175223393015	31.938261324450995	25.511748817645113
11	37.291035239633565	20.89903822030723	22.70628514328478	19.103641396774425
12	24.90442521146022	23.425137797440165	28.99409736938468	22.676339621714934
13	29.67151046256712	19.577899565120614	25.137170897937594	25.613419074374676
14	23.61425602034378	19.52089198344399	25.251121224641	31.61373077157123
15	24.966196757557753	27.400403877350684	22.206047911191824	25.427351453899742
16	25.62365418059415	25.67479131458732	23.87693138763122	24.824623117187308
17	24.04634774258395	25.99590157590225	25.218332627843555	24.739418053670246
18	24.870035416983605	24.97113529180979	26.13026083660947	24.028568454597135
19	25.754656904741697	24.838408861743964	25.25044024210513	24.156493991409207
20	25.97367450396153	24.3625336488393	24.90668001855457	24.757111828644604
21	26.71534101311891	24.171147371921577	24.505725676749744	24.607785938209773
22	26.00472295855348	24.38889250678712	24.833858403054975	24.772526131604426
23	24.637541909341838	24.31417073549594	25.490327956446844	25.557959398715376
24	25.104449520202117	24.8198322508945	25.223858528349364	24.851859700554023
25	25.07666660794899	24.502514451221888	25.37271047752954	25.048108463299577
26	24.798428855421815	25.22986370459578	25.536971436258465	24.43473600372394
27	25.271407378778843	24.921274911372553	24.795934000643786	25.011383709204814
28	24.621462647511464	24.742423781407325	25.56219436094781	25.073919210133404
29	24.80106589286944	24.868591658085823	25.28143297680797	25.04890947223677
30	24.729813840321395	24.975097926802317	25.532818957390496	24.76226927548579
31	25.374281664802712	24.681263362435974	24.724770519763727	25.219684452997587
32	25.018776533363514	24.90539669863971	24.604491741795044	25.471335026201736
33	24.578357115442206	24.675723992661187	25.268145924336753	25.477772967559854
34	25.30159713028996	24.624418157748647	25.350973651620617	24.723011060340777
35	25.54487833052376	24.68342875932685	25.263351187228896	24.508341722920495
36	24.788432250008942	25.151005430590352	25.082195532214403	24.978366787186303
37	25.4623564994678	24.87789176325895	24.832838494708376	24.826913242564878
38	24.82031286700803	24.921687158615804	25.24246552526723	25.015534449108934
39	25.524480190633692	24.630084058823563	24.717575271001227	25.127860479541525
40	25.692070521265485	24.76197898539739	25.218750867423523	24.327199625913597
41	24.92043580148719	24.992927033677994	25.465828231011088	24.620808933823728
42	25.653714962874393	25.327831661657868	24.902822186754353	24.115631188713387
43	25.04413143936017	24.592114471108655	25.211155659206536	25.152598430324634
44	24.806098929313787	25.104757288705216	24.767078507380784	25.322065274600213
45	24.868980551792315	25.282082816888778	24.863856140699248	24.98508049061966
46	24.86579106803994	24.816788860805442	25.039167069235635	25.278253001918987
47	25.160673526340847	24.75749507303439	25.16611827706735	24.91571312355741
48	25.573604402326712	25.285247518780295	24.45161748354439	24.689530595348604
49	25.008447212793435	25.25265573430967	24.598657306852413	25.14023974604449
50	24.94017520138658	25.558146821772542	24.60676838261395	24.894909594226927
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	23.0
1	28.0
2	33.0
3	95.0
4	157.0
5	200.0
6	243.0
7	243.0
8	243.0
9	320.5
10	398.0
11	607.5
12	817.0
13	1577.5
14	2338.0
15	3142.0
16	3946.0
17	4162.5
18	4379.0
19	4484.0
20	4589.0
21	5075.0
22	5561.0
23	6617.5
24	7674.0
25	9061.5
26	10449.0
27	13075.0
28	15701.0
29	18670.5
30	21640.0
31	25356.5
32	29073.0
33	34642.5
34	40212.0
35	46880.5
36	53549.0
37	61835.5
38	70122.0
39	75864.5
40	81607.0
41	89502.0
42	97397.0
43	100704.0
44	104011.0
45	113842.0
46	123673.0
47	131289.0
48	138905.0
49	143443.0
50	147981.0
51	144624.5
52	141268.0
53	139700.5
54	138133.0
55	137772.5
56	137412.0
57	132235.5
58	127059.0
59	116069.5
60	105080.0
61	91907.0
62	78734.0
63	68006.0
64	57278.0
65	48310.0
66	39342.0
67	33766.5
68	28191.0
69	25050.0
70	21909.0
71	16980.0
72	12051.0
73	10304.5
74	8558.0
75	6692.0
76	4826.0
77	4183.5
78	3541.0
79	2887.5
80	2234.0
81	1801.5
82	1369.0
83	1149.0
84	929.0
85	653.0
86	377.0
87	272.0
88	167.0
89	125.0
90	83.0
91	63.5
92	44.0
93	39.0
94	34.0
95	35.0
96	36.0
97	28.0
98	20.0
99	13.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00854060765355879
2	5.337879783474244E-4
3	2.668939891737122E-4
4	0.0
5	0.0
6	1.6013639350422732E-4
7	5.337879783474244E-5
8	0.0
9	0.001441227541538046
10	0.0012277123501990763
11	5.337879783474244E-5
12	5.337879783474244E-5
13	5.337879783474244E-4
14	2.668939891737122E-4
15	0.0033094854657540313
16	4.8040918051268206E-4
17	0.0
18	4.2703038267793953E-4
19	1.0675759566948488E-4
20	5.337879783474244E-4
21	0.0
22	5.337879783474244E-5
23	7.473031696863942E-4
24	5.337879783474244E-4
25	0.0017081215307117581
26	0.006352076942334351
27	0.005978425357491154
28	0.003950031039770941
29	0.0023486671047286676
30	0.0034696218592582587
31	0.007900062079541883
32	0.002882455083076092
33	0.004056788635440426
34	0.002295288306893925
35	0.0026155610939023795
36	0.0067257285271775475
37	0.0034696218592582587
38	0.00784668328170714
39	0.0038432734441014565
40	0.0020817731155549552
41	0.003896652241936199
42	0.0017615003285465006
43	0.0011743335523643338
44	0.001334469945868561
45	9.074395631906215E-4
46	9.608183610253641E-4
47	0.0020817731155549552
48	0.0016013639350422734
49	2.1351519133896977E-4
50	8.540607653558791E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1873403.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.696408794924245
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.61957542936483	46.1468673868538
2	13.023943404364797	15.28917412369107
3	3.8404671132977097	6.76264882936755
4	1.5605763277952889	3.664009043678159
5	0.8384062366685726	2.460571760185627
6	0.49715706638315443	1.7508800642226554
7	0.3283293995084935	1.3490229657059774
8	0.21913476708271834	1.0289939095894196
9	0.16415264698845475	0.8671653785162906
>10	0.7930731909458153	8.138006358255748
>50	0.055887606399275806	2.3065279634978055
>100	0.05106219616026218	6.289642086293881
>500	0.00622170914185387	2.4429732534534265
>1k	0.002012905898835075	1.503516876688477
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	5.337879783474244E-5
7	0.0	0.0	0.0	0.0	5.337879783474244E-5
8	0.0	0.0	0.0	0.0	5.337879783474244E-5
9	0.0	0.0	0.0	0.0	5.337879783474244E-5
10	0.0	0.0	0.0	0.0	5.337879783474244E-5
11	0.0	0.0	0.0	0.0	5.337879783474244E-5
12	0.0	0.0	0.0	0.0	1.0675759566948488E-4
13	0.0	0.0	0.0	0.0	1.6013639350422734E-4
14	0.0	0.0	0.0	0.0	1.6013639350422734E-4
15	0.0	0.0	0.0	0.0	2.668939891737122E-4
16	0.0	0.0	0.0	0.0	3.202727870084547E-4
17	0.0	0.0	0.0	0.0	3.202727870084547E-4
18	0.0	0.0	0.0	5.337879783474244E-5	3.202727870084547E-4
19	0.0	0.0	0.0	5.337879783474244E-5	3.202727870084547E-4
20	0.0	0.0	0.0	5.337879783474244E-5	4.2703038267793953E-4
21	0.0	0.0	0.0	5.337879783474244E-5	4.2703038267793953E-4
22	0.0	0.0	0.0	5.337879783474244E-5	4.2703038267793953E-4
23	0.0	0.0	0.0	1.0675759566948488E-4	4.80409180512682E-4
24	0.0	0.0	0.0	2.1351519133896977E-4	5.337879783474244E-4
25	0.0	0.0	0.0	3.736515848431971E-4	5.337879783474244E-4
26	0.0	0.0	0.0	4.2703038267793953E-4	5.871667761821669E-4
27	0.0	0.0	0.0	5.871667761821669E-4	5.871667761821669E-4
28	0.0	0.0	0.0	0.0012810911480338188	5.871667761821669E-4
29	0.0	0.0	0.0	0.003095970274415062	5.871667761821669E-4
30	0.0	0.0	0.0	0.005925046559656411	5.871667761821669E-4
31	0.0	0.0	0.0	0.014358896617545717	5.871667761821669E-4
32	0.0	0.0	0.0	0.022365716292757083	6.405455740169094E-4
33	0.0	0.0	0.0	0.032240793892184434	6.405455740169094E-4
34	0.0	0.0	0.0	0.045105084170357364	8.006819675211367E-4
35	0.0	0.0	0.0	0.06021128395758948	8.006819675211367E-4
36	0.0	0.0	0.0	0.0801215755499484	8.006819675211367E-4
37	0.0	0.0	0.0	0.1130562938139845	8.006819675211367E-4
38	0.0	0.0	0.0	0.15816137798434185	8.006819675211367E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2845	0.0	23.58628	1
TAGGACG	480	0.0	18.790995	4
AACGCAG	3825	0.0	17.656885	6
TGTAGGA	710	0.0	17.042099	2
GTGTATA	325	0.0	16.923826	1
CGAGCCG	555	0.0	16.648052	15
AGGACGT	550	0.0	16.399412	5
TTAGGAC	405	0.0	16.295713	3
CGTTTAT	530	0.0	16.188963	31
TCAACGC	4145	0.0	16.081451	4
ATCAACG	4190	0.0	15.908738	3
TATCAAC	4325	0.0	15.870377	2
CAACGCA	4230	0.0	15.810309	5
ATAACGC	555	0.0	15.458906	3
GGTATCA	1455	0.0	15.423363	1
ACGCAGA	4395	0.0	15.316858	7
TCGTTTA	550	0.0	15.200267	30
CGCAGAG	4460	0.0	15.192282	8
CAATACG	485	0.0	14.969736	38
GTAGGAC	735	0.0	14.96545	3
>>END_MODULE