FastQCFastQC Report
Thu 2 Feb 2017
SRR4064058_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064058_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529922
Sequences flagged as poor quality0
Sequence length25
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46730.881827891651979No Hit
GTCCTAAAGTGTGTATTTCTCATTT37400.7057642445491977No Hit
TATCAACGCAGAGTACTTTTTTTTT35220.6646261147867044No Hit
GTACTTTTTTTTTTTTTTTTTTTTT34220.6457554130607901No Hit
GTCCTACAGTGGACATTTCTAAATT32920.6212235008171014No Hit
CTTTAGGACGTGAAATATGGCGAGG31140.5876336517449737No Hit
CTGTAGGACGTGGAATATGGCAAGA28240.532908616739822No Hit
GTACATGGGGTGGTATCAACGCAAA25430.4798819448900027No Hit
GTCCTACAGTGTGCATTTCTCATTT24830.4685595238544541No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20860.393642838002574No Hit
CTGTAGGACCTGGAATATGGCGAGA17550.33118081528979737No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17490.3300485731862425No Hit
GGTATCAACGCAGAGTACTTTTTTT17150.3236325345994316No Hit
CTGAAGGACCTGGAATATGGCGAGA16220.30608278199433125No Hit
GATATACACTGTTCTACAAATCCCG16040.30268605568366663No Hit
ATTTAGAAATGTCCACTGTAGGACG15280.2883443223719717No Hit
GTCCTTCAGTGTGCATTTCTCATTT14200.2679639645079842No Hit
GTATCAACGCAGAGTACATGGGGTG13100.24720619260947835No Hit
TTGTAGAACAGTGTATATCAATGAG12960.24456429436785035No Hit
CCATAGGGTCTTCTCGTCTTATTAT12870.2428659312125181No Hit
TTTCTAAATTTTCCACCTTTTTCAG12780.24116756805718578No Hit
GTACATGGGTGGTATCAACGCAAAA12390.23380799438407918No Hit
GTATTAGAGGCACTGCCTGCCCAGT12220.23059997509067373No Hit
GTGTATATCAATGAGTTACAATGAG11690.2205985031759391No Hit
GAATATGGCAAGAAAACTGAAAATC10780.20342616460535703No Hit
GTTCTACAGTGTGGTTTTTATCATT9670.18247968568959205No Hit
TATCAACGCAGAGTACATGGGGTGG9560.18040390849974147No Hit
CTGTAGGACATGGAATATGGCAAGA9460.17851683832715004No Hit
GGAATATGGCGAGAAAACTGAAAAT9270.1749314049992263No Hit
CTCTAATACTTGTAATGCTAGAGGT9250.174553990964708No Hit
GCAGAGTACTTTTTTTTTTTTTTTT8660.16342027694641853No Hit
CCATTGGGATGTCCTGATCCAACAT8250.15568328923879363No Hit
GTACATGGGAGTGGTATCAACGCAA7790.14700276644487303No Hit
GTGTATATCAATGAGTTACAATGAA7620.1437947471514676No Hit
ATTCCAGGTCCTTCAGTGTGCATTT7590.14322862609969014No Hit
CTTCTACACCATTGGGATGTCCTGA7430.14020931382354385No Hit
GTTCTACAAATCCCGTTTCCAACGA7230.13643517347836095No Hit
GTTATATAATTTAAGCTCCATAGGG7120.13435939628851037No Hit
GCCATATTCCACGTCCTACAGTGGA7100.1339819822539921No Hit
ATCTATAACTTTATAGATGCAACAC7050.1330384471676964No Hit
CCATATTCCAGGTCCTTCAGTGTGC6860.12945301383977265No Hit
GTCAGGATACCGCGGCCGTTAAACT6830.12888689278799523No Hit
GGTATCAACGCAGAGTACATGGGGT6770.12775465068444036No Hit
ACCTATAACTTCTCTGTTAACCCAA6740.12718852963266292No Hit
CTGTAGAACATATTAGATGAGTGAG6700.12643370156362635No Hit
GATATATTTTGATCAACGGACCAAG6660.12567887349458978No Hit
CCTATAACTTCTCTGTTAACCCAAC6600.1245466313910349No Hit
ACAGTGGACATTTCTAAATTTTCCA6570.12398051033925747No Hit
CCATATTTCACGTCCTAAAGTGTGT6490.12247085420118434No Hit
TTCCAGGTCCTTCAGTGTGCATTTC6360.12001766297681546No Hit
CTGTTAGTATGAGTAACAAGAATTC6230.11756447175244658No Hit
GAAATATGGCGAGGAAAACTGAAAA6180.11662093666615088No Hit
CCTCTAGCATTACAAGTATTAGAGG6080.11473386649355943No Hit
GTTATAGATTAACCCAATTTTAAGT5870.11077101913111742No Hit
GTACATGGGAGAAATCGTAAATAGA5850.11039360509659912No Hit
GTCCACTGTAGGACGTGGAATATGG5830.11001619106208083No Hit
ACCTGGAATATGGCGAGAAAACTGA5730.10812912088948941No Hit
GTAGAACATATTAGATGAGTGAGTT5700.10756299983771198No Hit
GAAATACACACTTTAGGACGTGAAA5660.10680817176867538No Hit
ATACACACTTTAGGACGTGAAATAT5620.10605334369963881No Hit
GTATCCTGACCGTGCAAAGGTAGCA5600.10567592966512053No Hit
GATAGAAACCGACCTGGATTGCTCC5480.1034114454580108No Hit
GCCTAAAGGAAAGATCCAAAAAGAT5410.10209049633719679No Hit
ATAAATAATCCACCTATAACTTCTC5390.10171308230267852No Hit
GATATACACTGTTCTACAATGCCGG5300.10001471914734622No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAACTT401.2836965E-518.99811210
GGGTCCT350.002170468216.2825577
GTAAGAC350.00217320816.2794843
AACCTTG901.3460522E-1015.8302646
ACTCGGC551.1255606E-515.54537811
GTATAGT551.1255606E-515.5453781
AAGATCC551.1255606E-515.54537811
CGGTGTT508.669038E-515.20566814
GGTAAAA456.7328534E-414.78328815
TGTAGCA456.7812426E-414.7693362
GGCAAAC653.3490014E-614.62083415
CACCTTT3000.014.57209914
TCGGCAA602.5575151E-514.25262213
TTGTAGA2600.014.2499291
ACTATTC400.005276500714.2485848
GTCTGCA400.005276500714.2485849
CGGGAAA400.005276500714.2485849
CTAAAAC400.00528642714.2445493
CCTATGG602.5712201E-514.2445483
TAGGACC5650.014.1198234
Produced by FastQC (version 0.11.5)