##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064058_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	529922
Sequences flagged as poor quality	0
Sequence length	25
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.20211842497575	32.0	32.0	32.0	32.0	32.0
2	30.954304595770697	32.0	32.0	32.0	32.0	32.0
3	31.0071878502874	32.0	32.0	32.0	32.0	32.0
4	30.996750465162798	32.0	32.0	32.0	32.0	32.0
5	31.01958967546167	32.0	32.0	32.0	32.0	32.0
6	34.63629930442593	36.0	36.0	36.0	32.0	36.0
7	34.510778944825844	36.0	36.0	36.0	32.0	36.0
8	34.452430735089315	36.0	36.0	36.0	32.0	36.0
9	34.50728975207672	36.0	36.0	36.0	32.0	36.0
10	34.32484780779058	36.0	36.0	36.0	32.0	36.0
11	34.57401655337955	36.0	36.0	36.0	32.0	36.0
12	34.4243756628334	36.0	36.0	36.0	32.0	36.0
13	34.48822090798269	36.0	36.0	36.0	32.0	36.0
14	34.4469582315888	36.0	36.0	36.0	32.0	36.0
15	34.42814791610841	36.0	36.0	36.0	32.0	36.0
16	34.406539075562065	36.0	36.0	36.0	32.0	36.0
17	34.36807492423413	36.0	36.0	36.0	32.0	36.0
18	34.394301425492806	36.0	36.0	36.0	32.0	36.0
19	34.280794154611435	36.0	36.0	36.0	32.0	36.0
20	34.20412438056922	36.0	36.0	36.0	32.0	36.0
21	34.165833839697164	36.0	36.0	36.0	32.0	36.0
22	34.163246666490544	36.0	36.0	36.0	32.0	36.0
23	34.21171040266303	36.0	36.0	36.0	32.0	36.0
24	34.15615883092229	36.0	36.0	36.0	32.0	36.0
25	33.86286661055778	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	2.0
5	6.0
6	18.0
7	9.0
8	28.0
9	38.0
10	44.0
11	11.0
12	42.0
13	25.0
14	85.0
15	142.0
16	247.0
17	267.0
18	347.0
19	442.0
20	616.0
21	882.0
22	1214.0
23	1801.0
24	2685.0
25	3514.0
26	5171.0
27	6785.0
28	9264.0
29	12691.0
30	16533.0
31	23128.0
32	32123.0
33	46488.0
34	106605.0
35	258668.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.75250279871703	20.69955654729879	12.491056376449622	24.056884277534564
2	11.474435308393613	21.745038130483437	48.02497117331678	18.755555387806172
3	17.936529203850444	28.94475130823362	31.5193248703089	21.59939461760704
4	9.25263680333948	18.33297792237447	42.11299049071245	30.3013947835736
5	8.977124534554767	42.73980927361291	37.291104027599275	10.991962164233046
6	26.02245146776282	42.63495226987057	20.162157877453033	11.180438384913579
7	24.85225640473912	34.20780931780303	22.92606874630759	18.01386553115026
8	22.842062739975308	41.872269897421766	20.42069535736264	14.864972005240288
9	27.344797248056306	14.319698222640099	21.900192953241877	36.435311576061714
10	14.914872767026862	29.067310651619373	36.19507608299551	19.822740498358254
11	31.89959217581754	21.94188505399894	30.006419454723964	16.152103315459556
12	25.114428574665588	26.066111769904154	34.380499558147086	14.438960097283172
13	29.2235416741409	23.575662248842043	28.381014242905454	18.8197818341116
14	18.669403493797763	26.445567794065717	29.449720243142043	25.43530846899448
15	19.63701728812791	38.39924321956451	25.03115488319751	16.932584609110066
16	17.65523414235296	28.38580662460747	34.77380747812852	19.18515175491105
17	16.35728514647709	31.466659113867884	32.06314139783423	20.112914341820794
18	16.44149928616212	28.047219053806	38.88112522000559	16.630156440026287
19	22.948903400271174	25.819097868740393	30.037617128634718	21.194381602353715
20	22.191628188058885	29.57801006581494	31.806653258170215	16.42370848795596
21	21.45159676021309	26.138108286232793	28.698816522233408	23.71147843132071
22	21.531965199261986	32.08398406886119	29.199533925431847	17.18451680644497
23	18.98336134771195	32.11524296965004	31.636480386787287	17.264915295850724
24	22.22652377130447	29.210707709739864	32.11897455266512	16.443793966290542
25	19.6719330117879	30.620026212121786	33.44991558488157	16.25812519120874
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	381.0
1	381.0
2	630.0
3	879.0
4	879.0
5	879.0
6	2735.0
7	4591.0
8	4591.0
9	4591.0
10	4128.0
11	3665.0
12	3665.0
13	3665.0
14	3407.0
15	3149.0
16	3149.0
17	3149.0
18	5615.5
19	8082.0
20	8082.0
21	8082.0
22	14087.5
23	20093.0
24	20093.0
25	20093.0
26	32921.0
27	45749.0
28	45749.0
29	45749.0
30	59597.5
31	73446.0
32	73446.0
33	73446.0
34	73979.0
35	74512.0
36	74512.0
37	74512.0
38	70140.5
39	65769.0
40	65769.0
41	65769.0
42	62247.5
43	58726.0
44	58726.0
45	58726.0
46	62205.0
47	65684.0
48	65684.0
49	65684.0
50	57347.5
51	49011.0
52	49011.0
53	49011.0
54	36217.0
55	23423.0
56	23423.0
57	23423.0
58	18640.5
59	13858.0
60	13858.0
61	13858.0
62	11087.0
63	8316.0
64	8316.0
65	8316.0
66	6632.5
67	4949.0
68	4949.0
69	4949.0
70	3793.0
71	2637.0
72	2637.0
73	2637.0
74	2083.0
75	1529.0
76	1529.0
77	1529.0
78	1134.5
79	740.0
80	740.0
81	740.0
82	498.0
83	256.0
84	256.0
85	256.0
86	184.0
87	112.0
88	112.0
89	112.0
90	90.0
91	68.0
92	68.0
93	68.0
94	56.5
95	45.0
96	45.0
97	45.0
98	148.5
99	252.0
100	252.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.040572008710715916
2	0.005849917535033458
3	0.001698363155332294
4	0.0035854333279237323
5	0.010001471914734621
6	0.012832077173621777
7	0.02245613505383811
8	0.033967263106645884
9	0.04906382448737739
10	0.05717822622952057
11	0.053215378867078555
12	0.06302814376455403
13	0.061329780609221736
14	0.06699099112699605
15	0.058499175350334574
16	0.06623616305795947
17	0.05982012447114858
18	0.07359573673106608
19	0.07095383848943806
20	0.07680375602447152
21	0.0718973735757338
22	0.07416185778284351
23	0.08152143145595013
24	0.07491668585188009
25	0.07472797883462096
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	529922.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.554884624333226
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.35454916924746	18.55946831282593
2	14.780673533826208	8.145595077890338
3	6.048820694703107	5.000236690674697
4	3.0713248326030183	3.3852000562490283
5	1.8164179984284152	2.502559418812864
6	1.2015204529277523	1.9864654472520487
7	0.8403856071454453	1.6209709911390078
8	0.6258474977127915	1.3796124473522897
9	0.47323870870893375	1.1736034216418796
>10	3.0680517749897684	16.52082229475537
>50	0.37226802977213247	7.214654502154932
>100	0.296854616742787	16.51468438199802
>500	0.032907671140078004	5.98938548325056
>1k	0.01713941205212396	10.00674147400301
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4673	0.881827891651979	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3740	0.7057642445491977	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3522	0.6646261147867044	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3422	0.6457554130607901	No Hit
GTCCTACAGTGGACATTTCTAAATT	3292	0.6212235008171014	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3114	0.5876336517449737	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2824	0.532908616739822	No Hit
GTACATGGGGTGGTATCAACGCAAA	2543	0.4798819448900027	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2483	0.4685595238544541	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2086	0.393642838002574	No Hit
CTGTAGGACCTGGAATATGGCGAGA	1755	0.33118081528979737	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1749	0.3300485731862425	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1715	0.3236325345994316	No Hit
CTGAAGGACCTGGAATATGGCGAGA	1622	0.30608278199433125	No Hit
GATATACACTGTTCTACAAATCCCG	1604	0.30268605568366663	No Hit
ATTTAGAAATGTCCACTGTAGGACG	1528	0.2883443223719717	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	1420	0.2679639645079842	No Hit
GTATCAACGCAGAGTACATGGGGTG	1310	0.24720619260947835	No Hit
TTGTAGAACAGTGTATATCAATGAG	1296	0.24456429436785035	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	1287	0.2428659312125181	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	1278	0.24116756805718578	No Hit
GTACATGGGTGGTATCAACGCAAAA	1239	0.23380799438407918	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	1222	0.23059997509067373	No Hit
GTGTATATCAATGAGTTACAATGAG	1169	0.2205985031759391	No Hit
GAATATGGCAAGAAAACTGAAAATC	1078	0.20342616460535703	No Hit
GTTCTACAGTGTGGTTTTTATCATT	967	0.18247968568959205	No Hit
TATCAACGCAGAGTACATGGGGTGG	956	0.18040390849974147	No Hit
CTGTAGGACATGGAATATGGCAAGA	946	0.17851683832715004	No Hit
GGAATATGGCGAGAAAACTGAAAAT	927	0.1749314049992263	No Hit
CTCTAATACTTGTAATGCTAGAGGT	925	0.174553990964708	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	866	0.16342027694641853	No Hit
CCATTGGGATGTCCTGATCCAACAT	825	0.15568328923879363	No Hit
GTACATGGGAGTGGTATCAACGCAA	779	0.14700276644487303	No Hit
GTGTATATCAATGAGTTACAATGAA	762	0.1437947471514676	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	759	0.14322862609969014	No Hit
CTTCTACACCATTGGGATGTCCTGA	743	0.14020931382354385	No Hit
GTTCTACAAATCCCGTTTCCAACGA	723	0.13643517347836095	No Hit
GTTATATAATTTAAGCTCCATAGGG	712	0.13435939628851037	No Hit
GCCATATTCCACGTCCTACAGTGGA	710	0.1339819822539921	No Hit
ATCTATAACTTTATAGATGCAACAC	705	0.1330384471676964	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	686	0.12945301383977265	No Hit
GTCAGGATACCGCGGCCGTTAAACT	683	0.12888689278799523	No Hit
GGTATCAACGCAGAGTACATGGGGT	677	0.12775465068444036	No Hit
ACCTATAACTTCTCTGTTAACCCAA	674	0.12718852963266292	No Hit
CTGTAGAACATATTAGATGAGTGAG	670	0.12643370156362635	No Hit
GATATATTTTGATCAACGGACCAAG	666	0.12567887349458978	No Hit
CCTATAACTTCTCTGTTAACCCAAC	660	0.1245466313910349	No Hit
ACAGTGGACATTTCTAAATTTTCCA	657	0.12398051033925747	No Hit
CCATATTTCACGTCCTAAAGTGTGT	649	0.12247085420118434	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	636	0.12001766297681546	No Hit
CTGTTAGTATGAGTAACAAGAATTC	623	0.11756447175244658	No Hit
GAAATATGGCGAGGAAAACTGAAAA	618	0.11662093666615088	No Hit
CCTCTAGCATTACAAGTATTAGAGG	608	0.11473386649355943	No Hit
GTTATAGATTAACCCAATTTTAAGT	587	0.11077101913111742	No Hit
GTACATGGGAGAAATCGTAAATAGA	585	0.11039360509659912	No Hit
GTCCACTGTAGGACGTGGAATATGG	583	0.11001619106208083	No Hit
ACCTGGAATATGGCGAGAAAACTGA	573	0.10812912088948941	No Hit
GTAGAACATATTAGATGAGTGAGTT	570	0.10756299983771198	No Hit
GAAATACACACTTTAGGACGTGAAA	566	0.10680817176867538	No Hit
ATACACACTTTAGGACGTGAAATAT	562	0.10605334369963881	No Hit
GTATCCTGACCGTGCAAAGGTAGCA	560	0.10567592966512053	No Hit
GATAGAAACCGACCTGGATTGCTCC	548	0.1034114454580108	No Hit
GCCTAAAGGAAAGATCCAAAAAGAT	541	0.10209049633719679	No Hit
ATAAATAATCCACCTATAACTTCTC	539	0.10171308230267852	No Hit
GATATACACTGTTCTACAATGCCGG	530	0.10001471914734622	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAACTT	40	1.2836965E-5	18.998112	10
GGGTCCT	35	0.0021704682	16.282557	7
GTAAGAC	35	0.002173208	16.279484	3
AACCTTG	90	1.3460522E-10	15.830264	6
ACTCGGC	55	1.1255606E-5	15.545378	11
GTATAGT	55	1.1255606E-5	15.545378	1
AAGATCC	55	1.1255606E-5	15.545378	11
CGGTGTT	50	8.669038E-5	15.205668	14
GGTAAAA	45	6.7328534E-4	14.783288	15
TGTAGCA	45	6.7812426E-4	14.769336	2
GGCAAAC	65	3.3490014E-6	14.620834	15
CACCTTT	300	0.0	14.572099	14
TCGGCAA	60	2.5575151E-5	14.252622	13
TTGTAGA	260	0.0	14.249929	1
ACTATTC	40	0.0052765007	14.248584	8
GTCTGCA	40	0.0052765007	14.248584	9
CGGGAAA	40	0.0052765007	14.248584	9
CTAAAAC	40	0.005286427	14.244549	3
CCTATGG	60	2.5712201E-5	14.244548	3
TAGGACC	565	0.0	14.119823	4
>>END_MODULE