##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064058_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	529922
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.08960375300516	32.0	32.0	32.0	32.0	32.0
2	31.469435124414538	32.0	32.0	32.0	32.0	32.0
3	31.536056249787705	32.0	32.0	32.0	32.0	32.0
4	31.62449190635603	32.0	32.0	32.0	32.0	32.0
5	31.568840319896136	32.0	32.0	32.0	32.0	32.0
6	35.136184193145404	36.0	36.0	36.0	36.0	36.0
7	35.18038126365767	36.0	36.0	36.0	36.0	36.0
8	35.14500624620227	36.0	36.0	36.0	36.0	36.0
9	35.247028808013255	36.0	36.0	36.0	36.0	36.0
10	35.12782447228083	36.0	36.0	36.0	36.0	36.0
11	35.25587539298236	36.0	36.0	36.0	36.0	36.0
12	35.18642177528014	36.0	36.0	36.0	36.0	36.0
13	35.223391367031375	36.0	36.0	36.0	36.0	36.0
14	35.17741101520601	36.0	36.0	36.0	36.0	36.0
15	35.148038767969624	36.0	36.0	36.0	36.0	36.0
16	35.15318858247063	36.0	36.0	36.0	36.0	36.0
17	35.15730239544688	36.0	36.0	36.0	36.0	36.0
18	35.147165054479714	36.0	36.0	36.0	36.0	36.0
19	35.09668215322255	36.0	36.0	36.0	36.0	36.0
20	35.08699582202664	36.0	36.0	36.0	36.0	36.0
21	35.05412117254992	36.0	36.0	36.0	36.0	36.0
22	35.04145704462166	36.0	36.0	36.0	36.0	36.0
23	35.05165665890452	36.0	36.0	36.0	36.0	36.0
24	35.01392091666321	36.0	36.0	36.0	36.0	36.0
25	34.98867757896445	36.0	36.0	36.0	36.0	36.0
26	34.95595200803137	36.0	36.0	36.0	36.0	36.0
27	34.886798434486586	36.0	36.0	36.0	36.0	36.0
28	34.84688690033627	36.0	36.0	36.0	36.0	36.0
29	34.79932707077646	36.0	36.0	36.0	32.0	36.0
30	34.73301919905194	36.0	36.0	36.0	32.0	36.0
31	34.70617373877665	36.0	36.0	36.0	32.0	36.0
32	34.64084148233136	36.0	36.0	36.0	32.0	36.0
33	34.65772698623571	36.0	36.0	36.0	32.0	36.0
34	34.59062277089836	36.0	36.0	36.0	32.0	36.0
35	34.52123708772234	36.0	36.0	36.0	32.0	36.0
36	34.44621661301097	36.0	36.0	36.0	32.0	36.0
37	34.36513298183506	36.0	36.0	36.0	32.0	36.0
38	34.290820158438414	36.0	36.0	36.0	32.0	36.0
39	34.11859481206668	36.0	36.0	36.0	32.0	36.0
40	34.16424681368201	36.0	36.0	36.0	32.0	36.0
41	34.12602420733617	36.0	36.0	36.0	32.0	36.0
42	34.06114296066214	36.0	36.0	36.0	32.0	36.0
43	34.05819535705255	36.0	36.0	36.0	32.0	36.0
44	33.89396363993191	36.0	36.0	36.0	32.0	36.0
45	33.79780986635769	36.0	36.0	36.0	32.0	36.0
46	33.566041794830184	36.0	36.0	36.0	21.0	36.0
47	33.51481538792502	36.0	36.0	36.0	21.0	36.0
48	33.4031423492514	36.0	36.0	36.0	21.0	36.0
49	33.31305739335223	36.0	36.0	36.0	14.0	36.0
50	32.83727605194727	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	4.0
21	15.0
22	61.0
23	149.0
24	444.0
25	1078.0
26	2341.0
27	4539.0
28	7517.0
29	11259.0
30	15332.0
31	20674.0
32	29750.0
33	45508.0
34	89145.0
35	302106.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.87717146173227	21.58789171235268	13.085858252262284	24.449078573652773
2	11.299420102241655	22.46485796939544	46.93548661026832	19.300235318094582
3	17.596429649758456	29.22988375603865	32.036722524154584	21.136964070048307
4	9.023969565332257	18.98694524854601	42.298300504602565	29.690784681519165
5	8.810164514777647	42.950094542215645	37.0054460845181	11.234294858488608
6	26.341473540395643	42.669190313273106	19.731054251482767	11.258281894848478
7	24.59494038745325	34.4452579813633	23.29361679643419	17.666184834749266
8	22.772408014764437	41.85483901404358	20.379414328901234	14.993338642290752
9	26.93455919262371	14.215514215514217	21.979981657401012	36.86994493446106
10	14.908099335748792	29.300083031400963	35.94844504830918	19.843372584541065
11	31.715293411659474	21.91005829170763	30.24979195012087	16.124856346512026
12	24.911071650302592	26.205792938947504	34.326248629512705	14.556886781237202
13	29.147850897967427	23.417729879472144	28.662493701867643	18.771925520692783
14	18.686216787439612	26.33265398550725	29.498226147342994	25.482903079710145
15	19.805434987733534	37.93696923947915	25.228722400452913	17.028873372334402
16	17.617631407123366	28.457233005861283	34.473824252054094	19.451311334961257
17	16.37542883669672	31.47519823672163	31.9884813236665	20.160891602915147
18	16.6436443236715	27.647569444444443	38.95210597826087	16.756680253623188
19	23.055279833635893	25.616411471876994	30.72546525715105	20.60284343733606
20	22.30236055835159	29.5644412238143	31.90480773404137	16.228390483792747
21	21.337668562543165	25.69623454017761	29.545102864195105	23.420994033084114
22	21.640354618226834	31.532942584002928	29.610018078132256	17.216684719637986
23	18.99806950899858	31.670242698384843	32.060115074624896	17.271572717991685
24	22.41059482675654	28.82874552525952	32.226812022214716	16.533847625769223
25	19.87971587735322	30.23558628602485	33.483484050181914	16.401213786440014
26	15.709060989096017	31.69723229753651	35.64119887223579	16.952507841131688
27	17.309877069753572	32.069810188452784	32.7844814245868	17.83583131720684
28	16.299494627349002	32.050590104660074	31.619758976120295	20.030156291870636
29	19.021577086093217	28.314028314028317	30.537596666628925	22.126797933249545
30	17.207046546079013	29.8334607146564	32.64037893584699	20.319113803417594
31	21.17561801378795	27.59590324656575	31.787017133971084	19.441461605675215
32	17.213274077428974	32.402600466121285	31.959690887989357	18.424434568460384
33	16.88727349585395	32.30201056044325	33.80247668436805	17.008239259334744
34	18.34107365604283	31.027968953159885	34.29871921405373	16.332238176743555
35	17.823592685550377	32.74499443301693	32.10224377724519	17.329169104187503
36	16.3900270065733	30.85335864767085	33.85956204434664	18.89705230140921
37	17.706058964841134	31.16365262714919	29.364664498998884	21.76562390901079
38	18.365752439689516	34.272848911929614	29.92755004406633	17.433848604314537
39	20.291222561067666	30.362103324375735	29.652729551013014	19.693944563543585
40	21.942115463365017	30.5979993093258	31.605892998337477	15.853992228971705
41	20.925887834418102	28.381640005057434	33.521540328318046	17.170931832206417
42	18.884196303551143	34.3980343980344	30.656672592156465	16.061096706257995
43	15.644714172219423	34.6454256043871	32.17201939171606	17.537840831677414
44	16.07733242753623	33.36371527777778	30.90674064009662	19.652211654589372
45	16.51963217158784	33.63312644714967	31.15483251277077	18.692408868491718
46	19.05257039768417	29.65609773587612	29.854996433410452	21.43633543302926
47	22.18246734375767	30.776880776880777	28.85732401861434	18.183327860747216
48	17.533944123114082	32.259513318173674	28.452487663115782	21.754054895596465
49	20.03925120772947	30.749735809178745	29.335748792270532	19.875264190821255
50	16.998009114669333	34.84974005264995	28.043176735891606	20.109074096789108
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	140.0
1	90.5
2	41.0
3	154.5
4	268.0
5	277.0
6	286.0
7	270.0
8	254.0
9	377.5
10	501.0
11	749.5
12	998.0
13	1824.0
14	2650.0
15	3706.0
16	4762.0
17	5715.0
18	6668.0
19	6684.5
20	6701.0
21	7287.0
22	7873.0
23	8239.0
24	8605.0
25	9512.0
26	10419.0
27	14431.5
28	18444.0
29	22691.0
30	26938.0
31	29930.5
32	32923.0
33	40484.5
34	48046.0
35	56135.5
36	64225.0
37	64035.5
38	63846.0
39	56169.5
40	48493.0
41	43583.0
42	38673.0
43	31346.5
44	24020.0
45	22569.5
46	21119.0
47	19526.0
48	17933.0
49	15611.5
50	13290.0
51	12891.5
52	12493.0
53	11110.0
54	9727.0
55	9001.5
56	8276.0
57	8358.0
58	8440.0
59	7353.5
60	6267.0
61	5441.0
62	4615.0
63	3983.0
64	3351.0
65	3045.0
66	2739.0
67	2328.0
68	1917.0
69	1696.5
70	1476.0
71	1233.0
72	990.0
73	819.5
74	649.0
75	496.0
76	343.0
77	298.5
78	254.0
79	187.0
80	120.0
81	88.0
82	56.0
83	43.5
84	31.0
85	25.5
86	20.0
87	17.0
88	14.0
89	9.5
90	5.0
91	4.0
92	3.0
93	4.5
94	6.0
95	5.0
96	4.0
97	6.0
98	8.0
99	5.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00698215963858832
2	1.887070172591438E-4
3	3.774140345182876E-4
4	0.0
5	0.0
6	1.887070172591438E-4
7	0.0
8	0.0
9	0.0015096561380731504
10	3.774140345182876E-4
11	1.887070172591438E-4
12	1.887070172591438E-4
13	5.661210517774315E-4
14	3.774140345182876E-4
15	0.004151554379701164
16	7.548280690365752E-4
17	0.0
18	3.774140345182876E-4
19	0.0
20	9.43535086295719E-4
21	0.0
22	0.0
23	9.43535086295719E-4
24	5.661210517774315E-4
25	0.001887070172591438
26	0.004528968414219452
27	0.005283796483256027
28	0.003019312276146301
29	0.0015096561380731504
30	0.0032080192934054444
31	0.007359573673106607
32	0.0032080192934054444
33	0.003774140345182876
34	0.0024531912243688694
35	0.002264484207109726
36	0.009624057880216334
37	0.0024531912243688694
38	0.007359573673106607
39	0.003396726310664588
40	9.43535086295719E-4
41	0.001698363155332294
42	0.0015096561380731504
43	9.43535086295719E-4
44	3.774140345182876E-4
45	1.887070172591438E-4
46	7.548280690365752E-4
47	0.0015096561380731504
48	0.0013209491208140067
49	3.774140345182876E-4
50	0.0013209491208140067
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	529922.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.4052703388627
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.10514624387434	28.413227805630985
2	13.646664563769153	10.755010127182025
3	5.17048404416063	6.1123296462877725
4	2.6237941680198005	4.135652740174063
5	1.5042787186596807	2.9638254786891354
6	0.9620224558309403	2.274525296644488
7	0.6354306316552067	1.7527521075377392
8	0.4748538151167718	1.4969394364893376
9	0.35734572776536483	1.267317450632865
>10	2.1258376487443256	15.849935864130178
>50	0.20691253887484518	5.725004450983196
>100	0.16706627268739957	12.924060869099272
>500	0.01536241587930111	3.9068430731086705
>1k	0.004800754962281597	2.422575653410306
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1753	0.3308034012552791	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	1476	0.27853155747449626	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	1429	0.2696623276633165	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	1396	0.26343499609376475	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	1189	0.22437264352112196	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1184	0.22342910843482625	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	1156	0.21814531195157022	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	1153	0.2175791908997928	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	1062	0.2004068523292107	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	1008	0.19021667339721696	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	974	0.18380063481040607	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	836	0.1577590664286442	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA	832	0.15700423835960764	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT	815	0.1537962190662022	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	802	0.15134302784183332	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	779	0.14700276644487303	No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC	729	0.13756741558191582	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC	725	0.13681258751287925	No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC	710	0.1339819822539921	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	709	0.13379327523673296	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA	678	0.12794335770169948	No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC	653	0.1232256822702209	No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC	640	0.12077249104585203	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT	632	0.11926283490777889	No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA	626	0.11813059280422401	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	625	0.11794188578696488	No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC	617	0.11643222964889172	No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA	600	0.11322421035548627	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	593	0.11190326123467226	No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGAC	581	0.10963877702756256	No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC	568	0.10718558580319368	No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGG	565	0.10661946475141625	No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG	559	0.10548722264786137	No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC	559	0.10548722264786137	No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTC	554	0.10454368756156567	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA	552	0.10416627352704737	No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTT	551	0.10397756650978823	No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA	543	0.10246791037171508	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	5.661210517774314E-4	0.0
18	0.0	0.0	0.0	7.548280690365752E-4	0.0
19	0.0	0.0	0.0	0.0011322421035548627	0.0
20	0.0	0.0	0.0	0.0013209491208140067	0.0
21	0.0	0.0	0.0	0.0016983631553322942	0.0
22	0.0	0.0	0.0	0.0032080192934054444	0.0
23	0.0	0.0	0.0	0.008680522793920615	0.0
24	0.0	0.0	0.0	0.013775612259917497	0.0
25	0.0	0.0	0.0	0.017172338570582087	0.0
26	0.0	0.0	0.0	0.019625529794950955	0.0
27	0.0	0.0	0.0	0.0228335490883564	0.0
28	0.0	0.0	0.0	0.026985103468057565	0.0
29	0.0	0.0	0.0	0.03585433327923732	0.0
30	0.0	0.0	0.0	0.05868788236759372	0.0
31	0.0	0.0	0.0	0.10133566826816022	0.0
32	0.0	0.0	0.0	0.14983337170376018	0.0
33	0.0	0.0	0.0	0.19059408743173523	0.0
34	0.0	0.0	0.0	0.22814678386630485	0.0
35	0.0	0.0	0.0	0.2796637995780511	0.0
36	0.0	0.0	0.0	0.3415597012390503	0.0
37	0.0	0.0	0.0	0.43062941338536614	0.0
38	0.0	0.0	0.0	0.5466842289997396	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACTCCC	30	1.3002963E-4	36.665962	5
CGGTTAC	25	0.00234965	35.205967	1
CTAAGAC	25	0.0023518268	35.19932	3
CCCGGCG	25	0.0023518268	35.19932	18
TAAGACT	25	0.0023518268	35.19932	4
CGATTTT	45	4.0632E-5	29.335537	30
AAGTAGC	30	0.0057426807	29.332767	6
CGCAGTC	30	0.0057426807	29.332767	41
GGACGGG	30	0.0057426807	29.332767	6
GCCCGGC	30	0.0057426807	29.332767	17
AGCACTC	30	0.0057426807	29.332767	35
TAGGACC	715	0.0	27.691774	4
GTCCTAT	75	6.985465E-8	26.404472	1
GTTCCCC	50	8.3338455E-5	26.39949	9
CACATTG	45	0.0013965676	24.446281	28
GTAGGTC	45	0.0013973357	24.443974	3
TTGAGCA	45	0.0013973357	24.443974	4
AGACGGA	45	0.0013973357	24.443974	6
CTCGGAA	45	0.0013973357	24.443974	37
AGGACCT	1325	0.0	24.241041	5
>>END_MODULE