FastQCFastQC Report
Thu 2 Feb 2017
SRR4064057_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064057_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012741
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44780.44216635842727803No Hit
TATCAACGCAGAGTACTTTTTTTTT33670.33246407521765187No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23630.2333271784197539No Hit
GTACATGGGGTGGTATCAACGCAAA18150.17921660128305264No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17910.17684679498509492No Hit
GAGTACTTTTTTTTTTTTTTTTTTT16890.16677511821877458No Hit
GGTATCAACGCAGAGTACTTTTTTT16580.16371411841724587No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG14700.14515063574991038No Hit
GAATAGGACCGCGGTTCTATTTTGT14020.1384361845723635No Hit
GTATCAACGCAGAGTACATGGGGTG13720.13547392669991637No Hit
GATTAAGAGGGACGGCCGGGGGCAT13320.1315242495366535No Hit
GTATCTGATCGTCTTCGAACCTCCG12850.12688337886981962No Hit
GCGCAAGACGGACCAGAGCGAAAGC12690.12530350800451448No Hit
CTATTGGAGCTGGAATTACCGCGGC12490.12332866942288305No Hit
GAACTACGACGGTATCTGATCGTCT11750.11602176667084674No Hit
GTCCTATTCCATTATTCCTAGCTGC11590.1144418958055416No Hit
GAATAACGCCGCCGCATCGCCAGTC11520.1137507023019706No Hit
GTCTTGCGCCGGTCCAAGAATTTCA11440.11296076686931802No Hit
ATCAGATACCGTCGTAGTTCCGACC11340.1119733475785023No Hit
GTACATGGGGAATAATTGCAATCCC10820.10683876726626058No Hit
GGGTAGGCACACGCTGAGCCAGTCA10760.10624631569177115No Hit
CTTTAATATACGCTATTGGAGCTGG10310.10180292888310041No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTATAC508.7463224E-515.1924133
GTATAAT456.772387E-414.774051
GTATTAC400.005286260514.2464051
CGGTTCT1850.013.86684812
GGTTCTA1900.013.50193113
CGAACGA1351.8189894E-1213.37426716
ACTATAC500.001504567213.2933623
AGTATTA751.4729552E-512.67035915
CGCGGTC1501.8189894E-1212.66722810
CGGTCCA1650.012.66722710
AACCGCG1650.012.6659767
TACTATA751.4849797E-512.6603442
TAACGAA1405.2750693E-1112.21603413
GATATAC1251.4133548E-912.1569321
GGTCCTA1650.012.09263913
CCTTATA550.003072975212.0878591
TAAATCT550.003074112612.0872624
ATTAGGT550.00307866612.0848733
TATTACA951.044853E-611.9940112
CGTCGTA2550.011.92209610
Produced by FastQC (version 0.11.5)