##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064057_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1012741
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.158434387469253	32.0	32.0	32.0	32.0	32.0
2	30.75134313709033	32.0	32.0	32.0	32.0	32.0
3	30.77643247385067	32.0	32.0	32.0	32.0	32.0
4	30.79920433753546	32.0	32.0	32.0	32.0	32.0
5	30.70736545671598	32.0	32.0	32.0	32.0	32.0
6	34.34180802396664	36.0	36.0	36.0	32.0	36.0
7	34.250577393430305	36.0	36.0	36.0	32.0	36.0
8	34.18730356527483	36.0	36.0	36.0	32.0	36.0
9	34.31410301350493	36.0	36.0	36.0	32.0	36.0
10	34.005418957067995	36.0	36.0	36.0	32.0	36.0
11	34.35780224163928	36.0	36.0	36.0	32.0	36.0
12	34.127982376540494	36.0	36.0	36.0	32.0	36.0
13	34.23197737624921	36.0	36.0	36.0	32.0	36.0
14	34.111759077592396	36.0	36.0	36.0	32.0	36.0
15	34.04133929602929	36.0	36.0	36.0	32.0	36.0
16	34.060758871221765	36.0	36.0	36.0	32.0	36.0
17	33.96677334086405	36.0	36.0	36.0	32.0	36.0
18	33.971721298930326	36.0	36.0	36.0	32.0	36.0
19	33.97798746174985	36.0	36.0	36.0	32.0	36.0
20	33.955666848680956	36.0	36.0	36.0	32.0	36.0
21	33.93224131342564	36.0	36.0	36.0	32.0	36.0
22	33.92575495610428	36.0	36.0	36.0	32.0	36.0
23	33.87151206478261	36.0	36.0	36.0	32.0	36.0
24	33.86776678341254	36.0	36.0	36.0	32.0	36.0
25	33.46958699213323	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	1.0
4	8.0
5	11.0
6	46.0
7	11.0
8	56.0
9	74.0
10	93.0
11	41.0
12	71.0
13	55.0
14	118.0
15	195.0
16	304.0
17	440.0
18	586.0
19	773.0
20	1146.0
21	1783.0
22	2800.0
23	4345.0
24	6406.0
25	9226.0
26	12633.0
27	16889.0
28	22460.0
29	29741.0
30	38833.0
31	52437.0
32	72724.0
33	100534.0
34	210509.0
35	427391.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.004640976323905	17.26466901185138	11.545046019557695	25.185643992267014
2	16.740168148216906	19.872753234493096	37.41179328473612	25.97528533255388
3	18.37416228161557	24.723097476995147	28.698229818479128	28.20451042291016
4	12.166981992835051	15.31211365961363	35.90610521492869	36.614799132622636
5	14.422428392970883	37.03361000153064	33.071708249189	15.472253356309468
6	33.74777554152356	35.89787143271914	16.946769029612703	13.4075839961446
7	30.22087639455137	30.751339243386745	20.641928194431276	18.385856167630607
8	28.864066948119554	32.00443317930472	19.132704970336814	19.998794902238913
9	27.869249251147977	13.60934820235997	18.656195910754224	39.86520663573783
10	16.22335669351787	26.24330490129145	30.898874739285127	26.63446366590556
11	37.27547749933804	21.155502950161438	22.466773633895436	19.10224591660508
12	25.134748185160554	23.87871230632573	28.48302444586507	22.503515062648642
13	29.426469890756167	19.291772722242424	25.635740597283924	25.646016789717486
14	24.03833517288673	19.6783465955746	25.496407683239774	30.786910548298902
15	25.17058759596479	27.139878864527855	22.753411257892083	24.936122281615273
16	25.710333242755862	25.760134384032014	23.802079988142584	24.72745238506954
17	23.720893926728678	25.90660366732077	25.148161102844256	25.224341303106296
18	24.676554180386514	24.757094462386206	26.561196779179298	24.00515457804798
19	25.544984169272183	24.607690452944595	25.942728735241456	23.90459664254177
20	26.012667171983196	23.654446505459497	25.149149081882033	25.18373724067528
21	27.753644384012144	23.57169396660764	24.55295029091829	24.12171135846193
22	26.34685092438503	23.639808047308275	25.26316413615359	24.750176892153103
23	24.562268682087037	23.818888883398476	25.966606217232535	25.65223621728195
24	24.804679393552494	24.91120892239156	25.549793166694005	24.73431851736194
25	24.804060404044197	24.24393652061084	25.758583443931776	25.19341963141319
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	125.0
1	125.0
2	393.5
3	662.0
4	662.0
5	662.0
6	1827.0
7	2992.0
8	2992.0
9	2992.0
10	2919.0
11	2846.0
12	2846.0
13	2846.0
14	2641.0
15	2436.0
16	2436.0
17	2436.0
18	4470.0
19	6504.0
20	6504.0
21	6504.0
22	10747.5
23	14991.0
24	14991.0
25	14991.0
26	23003.5
27	31016.0
28	31016.0
29	31016.0
30	37755.5
31	44495.0
32	44495.0
33	44495.0
34	53952.0
35	63409.0
36	63409.0
37	63409.0
38	71787.0
39	80165.0
40	80165.0
41	80165.0
42	92000.5
43	103836.0
44	103836.0
45	103836.0
46	116767.0
47	129698.0
48	129698.0
49	129698.0
50	133447.0
51	137196.0
52	137196.0
53	137196.0
54	125510.5
55	113825.0
56	113825.0
57	113825.0
58	105135.0
59	96445.0
60	96445.0
61	96445.0
62	86116.0
63	75787.0
64	75787.0
65	75787.0
66	63098.5
67	50410.0
68	50410.0
69	50410.0
70	37943.5
71	25477.0
72	25477.0
73	25477.0
74	19914.0
75	14351.0
76	14351.0
77	14351.0
78	11673.0
79	8995.0
80	8995.0
81	8995.0
82	6180.5
83	3366.0
84	3366.0
85	3366.0
86	2638.5
87	1911.0
88	1911.0
89	1911.0
90	1358.0
91	805.0
92	805.0
93	805.0
94	568.0
95	331.0
96	331.0
97	331.0
98	499.0
99	667.0
100	667.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04482883580303355
2	0.006220741532139016
3	0.001974838581631434
4	0.0032584836596918658
5	0.009479225191830884
6	0.01333016042601218
7	0.022611901759679916
8	0.03702822340558939
9	0.05183951276782514
10	0.06121999603057446
11	0.05855396394537202
12	0.06595960862648989
13	0.06842815685352918
14	0.07109418893873162
15	0.06428099583210317
16	0.07069922122240534
17	0.06586086669740833
18	0.08146209149229665
19	0.07751241432903377
20	0.0823507688540308
21	0.07632751118005492
22	0.08146209149229665
23	0.0864979298754568
24	0.08047467220148094
25	0.08116586570505192
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1012741.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.446072638844
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.4539076354891	36.069994175546874
2	14.667725922981296	14.21187431022814
3	5.024248683888559	7.302153500858447
4	2.1494630599331286	4.16532174144129
5	1.0676654712990605	2.586209948826995
6	0.6441024812283835	1.872254135546995
7	0.4045557872117714	1.3719397337608334
8	0.2569982637406863	0.996044524259046
9	0.18950402419717774	0.8262653149448752
>10	0.9162088108255781	8.130854754072626
>50	0.09602493285761257	3.275325597329633
>100	0.11260222678031964	11.478894783051166
>500	0.012488610525362239	4.192519686550487
>1k	0.004504089041933922	3.5203477935825984
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4478	0.44216635842727803	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3367	0.33246407521765187	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2363	0.2333271784197539	No Hit
GTACATGGGGTGGTATCAACGCAAA	1815	0.17921660128305264	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1791	0.17684679498509492	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1689	0.16677511821877458	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1658	0.16371411841724587	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1470	0.14515063574991038	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1402	0.1384361845723635	No Hit
GTATCAACGCAGAGTACATGGGGTG	1372	0.13547392669991637	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1332	0.1315242495366535	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1285	0.12688337886981962	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1269	0.12530350800451448	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1249	0.12332866942288305	No Hit
GAACTACGACGGTATCTGATCGTCT	1175	0.11602176667084674	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1159	0.1144418958055416	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1152	0.1137507023019706	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1144	0.11296076686931802	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1134	0.1119733475785023	No Hit
GTACATGGGGAATAATTGCAATCCC	1082	0.10683876726626058	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1076	0.10624631569177115	No Hit
CTTTAATATACGCTATTGGAGCTGG	1031	0.10180292888310041	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.974838581631434E-4
13	0.0	0.0	0.0	0.0	4.937096454078585E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTATAC	50	8.7463224E-5	15.192413	3
GTATAAT	45	6.772387E-4	14.77405	1
GTATTAC	40	0.0052862605	14.246405	1
CGGTTCT	185	0.0	13.866848	12
GGTTCTA	190	0.0	13.501931	13
CGAACGA	135	1.8189894E-12	13.374267	16
ACTATAC	50	0.0015045672	13.293362	3
AGTATTA	75	1.4729552E-5	12.670359	15
CGCGGTC	150	1.8189894E-12	12.667228	10
CGGTCCA	165	0.0	12.667227	10
AACCGCG	165	0.0	12.665976	7
TACTATA	75	1.4849797E-5	12.660344	2
TAACGAA	140	5.2750693E-11	12.216034	13
GATATAC	125	1.4133548E-9	12.156932	1
GGTCCTA	165	0.0	12.092639	13
CCTTATA	55	0.0030729752	12.087859	1
TAAATCT	55	0.0030741126	12.087262	4
ATTAGGT	55	0.003078666	12.084873	3
TATTACA	95	1.044853E-6	11.994011	2
CGTCGTA	255	0.0	11.922096	10
>>END_MODULE