FastQCFastQC Report
Thu 2 Feb 2017
SRR4064057_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064057_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012741
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA14680.14495315189174726No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14680.14495315189174726No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14320.14139844244481067No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC13410.13241292689838766No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA13310.13142550760757193No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT12670.12510602414635136No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG11850.11700918596166247No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA11730.1158242828126836No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC11650.11503434738003103No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA11570.11424441194737844No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11460.11315825072748117No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG11370.11226957336574703No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA10820.10683876726626058No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10660.10525889640095541No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10250.10121047730861099No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCAAA450.001398194324.44403314
TAAGACC1004.6253263E-821.9985434
TTAACGG1601.8189894E-1220.6266935
GTACTAA654.9336057E-420.3063471
GTAATAC1101.2660166E-719.9986763
CTGTACG1051.793238E-618.8558949
TGTACGG600.007414122618.33211910
TACGACG5000.018.0388075
TAGGACG1102.7972837E-617.998814
GTATCAA34600.017.929451
CTAGATC750.00129124717.5988353
GTATAGA750.00129124717.5988351
TATACAG1509.598807E-917.5988355
ACGGTAT5150.017.5134039
GGCGTTA3650.017.4800242
CGACGGT5200.017.3450057
CTACGAC5400.017.1099784
ATAAGGG902.2160451E-417.1099783
ACGACGG5300.017.0177426
GACGGTA5350.016.8586988
Produced by FastQC (version 0.11.5)