##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064057_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1012741
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.257365901054662	32.0	32.0	32.0	32.0	32.0
2	31.413045388702542	32.0	32.0	32.0	32.0	32.0
3	31.483363466078693	32.0	32.0	32.0	32.0	32.0
4	31.56756367126442	32.0	32.0	32.0	32.0	32.0
5	31.509863825005603	32.0	32.0	32.0	32.0	32.0
6	35.08673491050525	36.0	36.0	36.0	36.0	36.0
7	35.10281799591406	36.0	36.0	36.0	36.0	36.0
8	35.06384653134415	36.0	36.0	36.0	36.0	36.0
9	35.1576553136488	36.0	36.0	36.0	36.0	36.0
10	35.01597446928682	36.0	36.0	36.0	36.0	36.0
11	35.16978575963647	36.0	36.0	36.0	36.0	36.0
12	35.06575718767187	36.0	36.0	36.0	36.0	36.0
13	35.11835207619717	36.0	36.0	36.0	36.0	36.0
14	35.05597186249989	36.0	36.0	36.0	36.0	36.0
15	35.0287269894277	36.0	36.0	36.0	36.0	36.0
16	35.02832807203421	36.0	36.0	36.0	36.0	36.0
17	34.98618797896007	36.0	36.0	36.0	36.0	36.0
18	34.99177479730751	36.0	36.0	36.0	36.0	36.0
19	34.98721094534535	36.0	36.0	36.0	36.0	36.0
20	34.97706224987435	36.0	36.0	36.0	36.0	36.0
21	34.97714124341761	36.0	36.0	36.0	36.0	36.0
22	34.96427714489687	36.0	36.0	36.0	36.0	36.0
23	34.92343945786731	36.0	36.0	36.0	32.0	36.0
24	34.888177727572995	36.0	36.0	36.0	32.0	36.0
25	34.85414039719928	36.0	36.0	36.0	32.0	36.0
26	34.80708986799191	36.0	36.0	36.0	32.0	36.0
27	34.800101901670814	36.0	36.0	36.0	32.0	36.0
28	34.784803814598206	36.0	36.0	36.0	32.0	36.0
29	34.74823177890497	36.0	36.0	36.0	32.0	36.0
30	34.7246196214037	36.0	36.0	36.0	32.0	36.0
31	34.7248664762264	36.0	36.0	36.0	32.0	36.0
32	34.67027996299152	36.0	36.0	36.0	32.0	36.0
33	34.63937077693112	36.0	36.0	36.0	32.0	36.0
34	34.61443152790299	36.0	36.0	36.0	32.0	36.0
35	34.57604165329536	36.0	36.0	36.0	32.0	36.0
36	34.557401151923344	36.0	36.0	36.0	32.0	36.0
37	34.540783872678205	36.0	36.0	36.0	32.0	36.0
38	34.49277258450088	36.0	36.0	36.0	32.0	36.0
39	34.49788445416943	36.0	36.0	36.0	32.0	36.0
40	34.47705583164896	36.0	36.0	36.0	32.0	36.0
41	34.44641818589353	36.0	36.0	36.0	32.0	36.0
42	34.354835046670374	36.0	36.0	36.0	32.0	36.0
43	34.344253861549994	36.0	36.0	36.0	32.0	36.0
44	34.25032856376902	36.0	36.0	36.0	32.0	36.0
45	34.20648813467609	36.0	36.0	36.0	32.0	36.0
46	34.160479332820536	36.0	36.0	36.0	32.0	36.0
47	34.11098691570698	36.0	36.0	36.0	32.0	36.0
48	34.032033856632644	36.0	36.0	36.0	32.0	36.0
49	34.01051700286648	36.0	36.0	36.0	32.0	36.0
50	33.40682267233182	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	2.0
20	10.0
21	39.0
22	124.0
23	311.0
24	875.0
25	1962.0
26	4094.0
27	7436.0
28	12383.0
29	18867.0
30	26655.0
31	37673.0
32	53875.0
33	85603.0
34	175474.0
35	587356.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.49107727432975	18.073666923750714	12.036866049994124	25.398389751925414
2	16.091755403185033	20.570513934529828	36.84701639914055	26.49071426314459
3	17.724954973458036	25.162332532861477	28.997487992922146	28.11522450075834
4	11.837676167944222	15.906238613821303	36.13678126984096	36.119303948393515
5	13.988176641411773	37.29749264619483	33.00162627957197	15.712704432821422
6	33.89096302206195	36.316563300119775	16.616127156157706	13.176346521660568
7	30.013132689535322	31.069474889902644	20.707980330588306	18.209412089973736
8	28.86315454790514	32.224922265416325	18.925174353561275	19.986748833117254
9	27.921053307186288	13.567234831653721	18.483826947648645	40.02788491351134
10	16.168328671031755	26.39264248326064	30.927697626126534	26.511331219581074
11	37.37520254438203	21.13827721006654	22.327919971641318	19.15860027391011
12	25.0481861089717	24.045263344984892	28.378260955427848	22.528289590615557
13	29.441573240068013	19.3123986657951	25.71869525978091	25.527332834355974
14	24.03437799002904	19.717775818299053	25.380230483410866	30.86761570826105
15	25.22319246443387	27.21442177327536	22.64787580811916	24.914509954171606
16	25.71973347446916	25.77611539433772	23.792182760364003	24.711968370829123
17	23.647507111887442	25.9855185086809	25.098618501670224	25.26835587776144
18	24.8238932478094	24.55156220068764	26.6717551824855	23.952789369017456
19	25.538835239054446	24.478839583703614	26.136816952031122	23.845508225210814
20	26.1111314083037	23.579160861395298	25.23666252274794	25.073045207553058
21	27.77225371541194	23.535237538521695	24.757860104409716	23.934648641656654
22	26.2788339345083	23.687643114366093	25.39203091813389	24.641492032991717
23	24.496264567003774	23.71412434064621	26.13568814713439	25.653922945215623
24	24.856724753538927	24.749490489130434	25.673522023508593	24.720262733822043
25	24.84418262033881	24.027573159123314	25.989015763426167	25.139228457111713
26	24.629402145576922	25.193050574313112	26.368931003428496	23.808616276681466
27	25.32302931136125	24.67948876198677	25.274642803889485	24.722839122762494
28	24.294978073445318	24.296261771760413	26.56366798031794	24.845092174476324
29	24.21433579208263	24.600231063186897	26.30160658036358	24.883826564366895
30	24.13466561206121	24.812358238018923	26.488272451592326	24.56470369832754
31	24.981089370745106	24.780528075618815	25.089220645074644	25.149161908561428
32	24.656441287674937	24.785994792186106	24.894713091322384	25.66285082881657
33	24.360569488633335	24.340227787553644	25.63024712204428	25.66895560176874
34	25.228002543674975	24.375733182714068	25.809710202743535	24.586554070867418
35	25.812545113966152	24.354382732323966	25.66887163490545	24.16420051880444
36	24.38644566564461	25.211990144815903	25.19470901038349	25.20685517915599
37	25.522515103219323	25.14332999571442	25.045768827429306	24.288386073636957
38	24.49775639506407	24.839827147619726	25.59911756805865	25.063298889257556
39	25.322751698927025	24.912560309193857	24.852127682685264	24.912560309193857
40	25.497621252905546	25.17206171905703	25.40954145181581	23.920775576221615
41	24.274647852554764	25.627940733572625	25.80044731242255	24.296964101450065
42	25.842856395221965	25.643195483042152	25.0056037372731	23.508344384462788
43	24.86156149158112	24.65716226731074	25.324372681994305	25.156903559113836
44	24.581997426765	25.366508250447055	24.75874687602754	25.2927474467604
45	24.688417689146412	25.689766513220647	24.906540118135663	24.71527567949728
46	24.642107404313474	25.41713816263698	24.820634046057506	25.120120386992046
47	24.944925275126764	25.15436228356448	25.221311030250366	24.679401411058393
48	25.958859065836947	25.7506067853313	23.891740412396786	24.398793736434964
49	24.711351515743967	26.07883389270857	24.308285369251838	24.901529222295622
50	24.8919507905835	25.96725889252152	24.277771140073444	24.86301917682153
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	26.0
1	22.0
2	18.0
3	59.5
4	101.0
5	125.0
6	149.0
7	168.5
8	188.0
9	268.5
10	349.0
11	537.5
12	726.0
13	1441.0
14	2156.0
15	3160.5
16	4165.0
17	4786.0
18	5407.0
19	5484.5
20	5562.0
21	5699.0
22	5836.0
23	6281.0
24	6726.0
25	6655.5
26	6585.0
27	7907.5
28	9230.0
29	10674.0
30	12118.0
31	13677.5
32	15237.0
33	17700.5
34	20164.0
35	23501.0
36	26838.0
37	32018.5
38	37199.0
39	40637.0
40	44075.0
41	47919.5
42	51764.0
43	52140.0
44	52516.0
45	58142.5
46	63769.0
47	68115.0
48	72461.0
49	75120.5
50	77780.0
51	73938.0
52	70096.0
53	69561.5
54	69027.0
55	71027.0
56	73027.0
57	71556.0
58	70085.0
59	63564.5
60	57044.0
61	50604.0
62	44164.0
63	38121.0
64	32078.0
65	27580.5
66	23083.0
67	20058.5
68	17034.0
69	15433.5
70	13833.0
71	10664.5
72	7496.0
73	6544.5
74	5593.0
75	4235.5
76	2878.0
77	2614.5
78	2351.0
79	1982.0
80	1613.0
81	1268.5
82	924.0
83	790.5
84	657.0
85	463.0
86	269.0
87	200.5
88	132.0
89	98.5
90	65.0
91	59.0
92	53.0
93	41.0
94	29.0
95	30.0
96	31.0
97	29.0
98	27.0
99	17.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010071676766320313
2	4.937096454078585E-4
3	4.937096454078585E-4
4	0.0
5	0.0
6	1.9748385816314342E-4
7	9.874192908157171E-5
8	0.0
9	6.911935035710018E-4
10	7.899354326525737E-4
11	0.0
12	9.874192908157171E-5
13	2.962257872447151E-4
14	0.0
15	0.004937096454078585
16	4.937096454078585E-4
17	0.0
18	2.962257872447151E-4
19	9.874192908157171E-5
20	0.0011849031489788605
21	0.0
22	1.9748385816314342E-4
23	6.911935035710018E-4
24	4.937096454078585E-4
25	0.0016786127943867188
26	0.0052333222413233
27	0.006121999603057445
28	0.004147161021426011
29	0.0030609998015287223
30	0.003949677163262868
31	0.00819558011377045
32	0.0031597417306102947
33	0.0042459029505075825
34	0.0032584836596918658
35	0.0027647740142840074
36	0.007504386610199449
37	0.0042459029505075825
38	0.008393063971933594
39	0.0042459029505075825
40	0.002271064368876149
41	0.002567290156120864
42	0.002369806297957721
43	0.0016786127943867188
44	7.899354326525737E-4
45	6.911935035710018E-4
46	6.911935035710018E-4
47	0.002567290156120864
48	0.002666032085202436
49	3.9496771632628684E-4
50	7.899354326525737E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1012741.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.251411759468155
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.9549129647799	38.41545107753033
2	14.665573200337734	15.032626615582606
3	4.8124168103920635	7.399294665227702
4	2.074738711365962	4.25333151958101
5	1.0402482452458601	2.665709557457989
6	0.594536364097572	1.8282496801405046
7	0.36753405011754764	1.3185647256739608
8	0.24835887793423886	1.0182994493701734
9	0.18077014175774958	0.8338252472139467
>10	0.8611325396507181	7.910102846561802
>50	0.08933322175345776	3.2444812580329865
>100	0.09688146734739464	10.448207989946932
>500	0.01046319831242169	3.700547651665059
>1k	0.003100206907384204	1.9313077160149759
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1468	0.14495315189174726	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1468	0.14495315189174726	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1432	0.14139844244481067	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1341	0.13241292689838766	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1331	0.13142550760757193	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1267	0.12510602414635136	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1185	0.11700918596166247	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1173	0.1158242828126836	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1165	0.11503434738003103	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	1157	0.11424441194737844	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1146	0.11315825072748117	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1137	0.11226957336574703	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	1082	0.10683876726626058	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA	1066	0.10525889640095541	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1025	0.10121047730861099	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	9.87419290815717E-5
7	0.0	0.0	0.0	0.0	1.974838581631434E-4
8	0.0	0.0	0.0	0.0	1.974838581631434E-4
9	0.0	0.0	0.0	0.0	1.974838581631434E-4
10	0.0	0.0	0.0	0.0	1.974838581631434E-4
11	0.0	0.0	0.0	0.0	1.974838581631434E-4
12	0.0	0.0	0.0	0.0	3.949677163262868E-4
13	0.0	0.0	0.0	0.0	4.937096454078585E-4
14	0.0	0.0	0.0	0.0	4.937096454078585E-4
15	0.0	0.0	0.0	0.0	4.937096454078585E-4
16	0.0	0.0	0.0	0.0	4.937096454078585E-4
17	0.0	0.0	0.0	0.0	4.937096454078585E-4
18	0.0	0.0	0.0	0.0	5.924515744894301E-4
19	0.0	0.0	0.0	0.0	5.924515744894301E-4
20	0.0	0.0	0.0	0.0	5.924515744894301E-4
21	0.0	0.0	0.0	9.87419290815717E-5	5.924515744894301E-4
22	0.0	0.0	0.0	2.9622578724471506E-4	5.924515744894301E-4
23	0.0	0.0	0.0	9.87419290815717E-4	5.924515744894301E-4
24	0.0	0.0	0.0	0.0017773547234682906	5.924515744894301E-4
25	0.0	0.0	0.0	0.001974838581631434	5.924515744894301E-4
26	0.0	0.0	0.0	0.002271064368876149	7.899354326525736E-4
27	0.0	0.0	0.0	0.0027647740142840074	7.899354326525736E-4
28	0.0	0.0	0.0	0.0040484190923444395	7.899354326525736E-4
29	0.0	0.0	0.0	0.005727031886731159	7.899354326525736E-4
30	0.0	0.0	0.0	0.010565386411728172	7.899354326525736E-4
31	0.0	0.0	0.0	0.021821966327027344	8.886773617341453E-4
32	0.0	0.0	0.0	0.03327603010048966	8.886773617341453E-4
33	0.0	0.0	0.0	0.04482883580303355	8.886773617341453E-4
34	0.0	0.0	0.0	0.05628289957649587	8.886773617341453E-4
35	0.0	0.0	0.0	0.07129167279689476	8.886773617341453E-4
36	0.0	0.0	0.0	0.0941998003438194	8.886773617341453E-4
37	0.0	0.0	0.0	0.1298456367422668	8.886773617341453E-4
38	0.0	0.0	0.0	0.17763673041774747	8.886773617341453E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCAAA	45	0.0013981943	24.444033	14
TAAGACC	100	4.6253263E-8	21.998543	4
TTAACGG	160	1.8189894E-12	20.62669	35
GTACTAA	65	4.9336057E-4	20.306347	1
GTAATAC	110	1.2660166E-7	19.998676	3
CTGTACG	105	1.793238E-6	18.855894	9
TGTACGG	60	0.0074141226	18.332119	10
TACGACG	500	0.0	18.038807	5
TAGGACG	110	2.7972837E-6	17.99881	4
GTATCAA	3460	0.0	17.92945	1
CTAGATC	75	0.001291247	17.598835	3
GTATAGA	75	0.001291247	17.598835	1
TATACAG	150	9.598807E-9	17.598835	5
ACGGTAT	515	0.0	17.513403	9
GGCGTTA	365	0.0	17.48002	42
CGACGGT	520	0.0	17.345005	7
CTACGAC	540	0.0	17.109978	4
ATAAGGG	90	2.2160451E-4	17.109978	3
ACGACGG	530	0.0	17.017742	6
GACGGTA	535	0.0	16.858698	8
>>END_MODULE