FastQCFastQC Report
Thu 2 Feb 2017
SRR4064056_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064056_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1695212
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39360.23218334933919768No Hit
TATCAACGCAGAGTACTTTTTTTTT27130.1600389803753159No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19630.1157967263091578No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGAC500.00149938213.3003111
TCGCGTA801.997194E-613.0639619
CGAACGA1352.7284841E-1112.66883116
ATAATAC853.957215E-612.2904133
GTATCTA853.958521E-612.29005051
GATAACG1405.2750693E-1112.2145711
AAATCCG550.003064716512.09297618
CGCATCG2200.012.09154813
CGTAAAT802.8755729E-511.8714213
CGATAAC1459.640644E-1111.79372710
ATAACGA1552.5465852E-1111.64577712
CGCGTAA907.457171E-611.61172510
CGACCAT2300.011.56582810
ATCGCCA2250.011.40194916
CGAGCCG3250.011.40161215
TAACGAA1501.7644197E-1011.40060213
TTAGGAC2450.011.2415093
TCGCACG1701.0913936E-1111.17706113
CCGATAA1451.2150849E-911.1391769
TCGTCGC600.00587371811.0839213
Produced by FastQC (version 0.11.5)