##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064056_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1695212
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22884040462196	32.0	32.0	32.0	32.0	32.0
2	30.76651061932077	32.0	32.0	32.0	32.0	32.0
3	30.785598497415073	32.0	32.0	32.0	32.0	32.0
4	30.822202178842527	32.0	32.0	32.0	32.0	32.0
5	30.703746788012356	32.0	32.0	32.0	32.0	32.0
6	34.37414907398013	36.0	36.0	36.0	32.0	36.0
7	34.31534109008195	36.0	36.0	36.0	32.0	36.0
8	34.25550255661239	36.0	36.0	36.0	32.0	36.0
9	34.431962492006896	36.0	36.0	36.0	32.0	36.0
10	34.05654514007688	36.0	36.0	36.0	32.0	36.0
11	34.42129303001631	36.0	36.0	36.0	32.0	36.0
12	34.19498269243021	36.0	36.0	36.0	32.0	36.0
13	34.300167176730696	36.0	36.0	36.0	32.0	36.0
14	34.17977515496587	36.0	36.0	36.0	32.0	36.0
15	34.104585739128794	36.0	36.0	36.0	32.0	36.0
16	34.112123439428224	36.0	36.0	36.0	32.0	36.0
17	34.024503129992	36.0	36.0	36.0	32.0	36.0
18	34.02720131759332	36.0	36.0	36.0	32.0	36.0
19	34.04569870907002	36.0	36.0	36.0	32.0	36.0
20	34.02843479163668	36.0	36.0	36.0	32.0	36.0
21	34.01610005120303	36.0	36.0	36.0	32.0	36.0
22	33.98769652409256	36.0	36.0	36.0	32.0	36.0
23	33.92937874436944	36.0	36.0	36.0	32.0	36.0
24	33.916446438557536	36.0	36.0	36.0	32.0	36.0
25	33.48468215184885	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	8.0
4	16.0
5	17.0
6	51.0
7	26.0
8	109.0
9	105.0
10	139.0
11	50.0
12	105.0
13	101.0
14	158.0
15	294.0
16	497.0
17	650.0
18	814.0
19	1153.0
20	1821.0
21	2735.0
22	4188.0
23	6300.0
24	9762.0
25	14148.0
26	20162.0
27	26597.0
28	36414.0
29	48424.0
30	63963.0
31	87010.0
32	121833.0
33	170131.0
34	356764.0
35	720666.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.56030285683936	17.110349182369152	11.35606923453701	25.973278726254478
2	16.59238485040301	19.555772124821768	37.798953585024314	26.05288943975091
3	18.698560156301617	23.859710804254878	28.635298587056035	28.80643045238747
4	12.428929257843887	15.476579543188713	36.17407863176064	35.92041256720676
5	14.487206412958065	36.76755150825808	33.76731788931181	14.977924189472045
6	34.115041480679125	35.74064044611052	16.912447507560447	13.231870565649913
7	30.140698643166154	30.494185840687077	20.91268258489947	18.4524329312473
8	28.102546853609027	33.24175046027475	19.29914318085257	19.356559505263654
9	27.576747655785226	13.858428785918658	18.390164213070946	40.174659345225166
10	15.673982486418591	26.961601390131673	31.906862594644874	25.457553528804862
11	37.20869014517611	20.989065016729754	22.62279058978792	19.179454248306218
12	24.567113557174064	23.616253805003215	29.20908466836781	22.60754796945491
13	29.687604221944262	19.538504492943453	25.16975079998182	25.60414048513046
14	23.447338703917197	19.6402924837209	25.431113613999173	31.481255198362735
15	25.088079763759126	27.48047311921889	22.290658203296772	25.140788913725213
16	25.580518132023496	25.693738131232486	24.12288443000696	24.602859306737063
17	23.909522347885627	25.948516043727714	25.326246074658233	24.815715533728426
18	24.53236530910675	25.0805686532298	26.397531845206647	23.9895341924568
19	25.60183477031427	24.81963700120134	25.325379513502504	24.253148714981894
20	25.780456227207978	24.38534957956701	25.061383865976243	24.772810327248767
21	26.75407706952787	24.25556031111177	24.39995867585637	24.59040394350399
22	25.74473812846688	24.57883774803942	24.91002925820186	24.766394865291836
23	24.416153179018412	24.40239720062888	25.534047817670114	25.647401802682595
24	24.896333904008504	25.001003601157095	25.300667099592655	24.80199539524175
25	24.869395396176646	24.615182073060648	25.394057217479908	25.121365313282794
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	132.0
1	132.0
2	354.0
3	576.0
4	576.0
5	576.0
6	1717.0
7	2858.0
8	2858.0
9	2858.0
10	3022.5
11	3187.0
12	3187.0
13	3187.0
14	3864.0
15	4541.0
16	4541.0
17	4541.0
18	7872.5
19	11204.0
20	11204.0
21	11204.0
22	18054.5
23	24905.0
24	24905.0
25	24905.0
26	37287.0
27	49669.0
28	49669.0
29	49669.0
30	62361.0
31	75053.0
32	75053.0
33	75053.0
34	91948.5
35	108844.0
36	108844.0
37	108844.0
38	123883.0
39	138922.0
40	138922.0
41	138922.0
42	159291.0
43	179660.0
44	179660.0
45	179660.0
46	199028.5
47	218397.0
48	218397.0
49	218397.0
50	223272.0
51	228147.0
52	228147.0
53	228147.0
54	213283.0
55	198419.0
56	198419.0
57	198419.0
58	182151.5
59	165884.0
60	165884.0
61	165884.0
62	144742.0
63	123600.0
64	123600.0
65	123600.0
66	101510.5
67	79421.0
68	79421.0
69	79421.0
70	59509.0
71	39597.0
72	39597.0
73	39597.0
74	30327.0
75	21057.0
76	21057.0
77	21057.0
78	16476.5
79	11896.0
80	11896.0
81	11896.0
82	8164.5
83	4433.0
84	4433.0
85	4433.0
86	3419.0
87	2405.0
88	2405.0
89	2405.0
90	1708.5
91	1012.0
92	1012.0
93	1012.0
94	733.5
95	455.0
96	455.0
97	455.0
98	696.5
99	938.0
100	938.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04023095636415976
2	0.0053680601600271825
3	0.0016517108184699023
4	0.004129277046174756
5	0.008494512780702354
6	0.012151872450171424
7	0.022652034081872947
8	0.033742092434456576
9	0.048843448489038536
10	0.05804583733479942
11	0.054565446681594985
12	0.06258804208559166
13	0.0655375256900022
14	0.06895892667111841
15	0.059520579137004696
16	0.06872296798276557
17	0.06441672192032619
18	0.0775714187959972
19	0.07473991453576308
20	0.08040292305623131
21	0.0759197079775273
22	0.07833828453314394
23	0.08276250993975975
24	0.07739444977973256
25	0.08016696436787847
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1695212.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.362041816107485
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.05074199056124	40.58222874246329
2	14.094123939306522	15.041824620213523
3	4.450898733545533	7.125271330161498
4	1.8851923630725864	4.023908548387433
5	0.9923294783164396	2.6476363558638987
6	0.5801364912847863	1.857436062419318
7	0.378247598047831	1.4128844900709399
8	0.2601870925492898	1.1107291610101302
9	0.1961185424272643	0.9418757275725954
>10	0.957026517661839	9.179299012830798
>50	0.0720988653130724	2.692458351621524
>100	0.0709926224332143	7.9071928071377835
>500	0.0087902380650072	3.0809855071756673
>1k	0.0031155274154455893	2.3962692830717978
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3936	0.23218334933919768	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2713	0.1600389803753159	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1963	0.1157967263091578	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.8989672088210796E-5
2	0.0	0.0	0.0	0.0	5.8989672088210796E-5
3	0.0	0.0	0.0	0.0	5.8989672088210796E-5
4	0.0	0.0	0.0	0.0	5.8989672088210796E-5
5	0.0	0.0	0.0	0.0	5.8989672088210796E-5
6	0.0	0.0	0.0	0.0	1.769690162646324E-4
7	0.0	0.0	0.0	0.0	1.769690162646324E-4
8	0.0	0.0	0.0	0.0	1.769690162646324E-4
9	0.0	0.0	0.0	0.0	1.769690162646324E-4
10	0.0	0.0	0.0	0.0	2.3595868835284318E-4
11	0.0	0.0	0.0	0.0	2.3595868835284318E-4
12	0.0	0.0	0.0	0.0	2.3595868835284318E-4
13	0.0	0.0	0.0	0.0	2.9494836044105397E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTCGAC	50	0.001499382	13.30031	11
TCGCGTA	80	1.997194E-6	13.063961	9
CGAACGA	135	2.7284841E-11	12.668831	16
ATAATAC	85	3.957215E-6	12.290413	3
GTATCTA	85	3.958521E-6	12.2900505	1
GATAACG	140	5.2750693E-11	12.21457	11
AAATCCG	55	0.0030647165	12.092976	18
CGCATCG	220	0.0	12.091548	13
CGTAAAT	80	2.8755729E-5	11.871421	3
CGATAAC	145	9.640644E-11	11.793727	10
ATAACGA	155	2.5465852E-11	11.645777	12
CGCGTAA	90	7.457171E-6	11.611725	10
CGACCAT	230	0.0	11.565828	10
ATCGCCA	225	0.0	11.401949	16
CGAGCCG	325	0.0	11.401612	15
TAACGAA	150	1.7644197E-10	11.400602	13
TTAGGAC	245	0.0	11.241509	3
TCGCACG	170	1.0913936E-11	11.177061	13
CCGATAA	145	1.2150849E-9	11.139176	9
TCGTCGC	60	0.005873718	11.08392	13
>>END_MODULE