##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064056_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1695212
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.299186178483872	32.0	32.0	32.0	32.0	32.0
2	31.40453819345309	32.0	32.0	32.0	32.0	32.0
3	31.484885666217558	32.0	32.0	32.0	32.0	32.0
4	31.572311899632613	32.0	32.0	32.0	32.0	32.0
5	31.517414930993883	32.0	32.0	32.0	32.0	32.0
6	35.11257176093609	36.0	36.0	36.0	36.0	36.0
7	35.134553672343046	36.0	36.0	36.0	36.0	36.0
8	35.08191541824857	36.0	36.0	36.0	36.0	36.0
9	35.190010452969894	36.0	36.0	36.0	36.0	36.0
10	35.04418562398095	36.0	36.0	36.0	36.0	36.0
11	35.190352593068006	36.0	36.0	36.0	36.0	36.0
12	35.09157615684646	36.0	36.0	36.0	36.0	36.0
13	35.13401155725656	36.0	36.0	36.0	36.0	36.0
14	35.081475945191514	36.0	36.0	36.0	36.0	36.0
15	35.04903988409709	36.0	36.0	36.0	36.0	36.0
16	35.06615809704037	36.0	36.0	36.0	36.0	36.0
17	35.02817405728605	36.0	36.0	36.0	36.0	36.0
18	35.02480043793933	36.0	36.0	36.0	36.0	36.0
19	35.01763614226421	36.0	36.0	36.0	36.0	36.0
20	35.0186867483241	36.0	36.0	36.0	36.0	36.0
21	35.012720532889105	36.0	36.0	36.0	36.0	36.0
22	34.99724990148725	36.0	36.0	36.0	36.0	36.0
23	34.94391910864246	36.0	36.0	36.0	36.0	36.0
24	34.91685995615887	36.0	36.0	36.0	32.0	36.0
25	34.88053883526072	36.0	36.0	36.0	32.0	36.0
26	34.833393109534384	36.0	36.0	36.0	32.0	36.0
27	34.82550678027291	36.0	36.0	36.0	32.0	36.0
28	34.802229455666904	36.0	36.0	36.0	32.0	36.0
29	34.776150121636704	36.0	36.0	36.0	32.0	36.0
30	34.75220857332298	36.0	36.0	36.0	32.0	36.0
31	34.75113791077458	36.0	36.0	36.0	32.0	36.0
32	34.707841261152	36.0	36.0	36.0	32.0	36.0
33	34.68511136070297	36.0	36.0	36.0	32.0	36.0
34	34.67360601505889	36.0	36.0	36.0	32.0	36.0
35	34.63384756596815	36.0	36.0	36.0	32.0	36.0
36	34.60833335299656	36.0	36.0	36.0	32.0	36.0
37	34.59455808477052	36.0	36.0	36.0	32.0	36.0
38	34.55025507134211	36.0	36.0	36.0	32.0	36.0
39	34.54829012536485	36.0	36.0	36.0	32.0	36.0
40	34.52330504975189	36.0	36.0	36.0	32.0	36.0
41	34.51176431030455	36.0	36.0	36.0	32.0	36.0
42	34.450281734673894	36.0	36.0	36.0	32.0	36.0
43	34.44199840491927	36.0	36.0	36.0	32.0	36.0
44	34.37646028933254	36.0	36.0	36.0	32.0	36.0
45	34.33760438222476	36.0	36.0	36.0	32.0	36.0
46	34.319951132955644	36.0	36.0	36.0	32.0	36.0
47	34.286141202398284	36.0	36.0	36.0	32.0	36.0
48	34.23938657819789	36.0	36.0	36.0	32.0	36.0
49	34.22087679889005	36.0	36.0	36.0	32.0	36.0
50	33.63092285802602	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	0.0
19	3.0
20	8.0
21	67.0
22	179.0
23	513.0
24	1379.0
25	3135.0
26	6181.0
27	11466.0
28	18969.0
29	29273.0
30	42128.0
31	59766.0
32	86468.0
33	138687.0
34	291151.0
35	1005836.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.53925624222745	18.022580805017853	11.961467032591024	26.476695920163674
2	15.753697789407989	20.33728133874311	37.36439669136775	26.544624180481147
3	17.92832257554539	24.429951286156374	29.019776947462432	28.621949190835807
4	12.0264014176398	16.05893540158989	36.324542299134265	35.59012088163605
5	14.051752819116428	37.12786365363152	33.677793691880424	15.142589835371624
6	34.186560957049565	36.0846738752131	16.660590723273224	13.068174444464109
7	30.026468681479766	30.56451379798739	20.94948652409641	18.45953099643643
8	28.006644596664014	33.420775690592095	19.199840491926672	19.37273922081722
9	27.53752540283214	13.68405218309506	18.356698531428723	40.42172388264408
10	15.554961718132903	27.138271897705803	32.03389120623128	25.27287517793001
11	37.362406589854245	20.874616272183065	22.628379223365574	19.134597914597112
12	24.558963196300162	23.583036909881372	29.20953746143546	22.64846243238301
13	29.630628090339677	19.49685052282855	25.283653511498926	25.58886787533285
14	23.421300854348935	19.65061535185337	25.357463298035814	31.57062049576188
15	24.975252810678988	27.525127688417843	22.218020673194342	25.28159882770883
16	25.474737214053082	25.664036300120163	24.067706425373746	24.79352006045301
17	23.882572729884362	25.998061598231725	25.352891174019042	24.76647449786487
18	24.68347523340807	25.0396559240258	26.36893311554282	23.907935727023307
19	25.616737021682244	24.841199802738537	25.406320861343595	24.13574231423562
20	25.838812548888214	24.347006891246668	25.083500767463786	24.730679792401343
21	26.73594807021187	24.14771721766953	24.515163885106993	24.601170827011607
22	25.774623792922412	24.46923979919892	25.000619392287682	24.75551701559099
23	24.448782179812305	24.28302012563702	25.58959085087845	25.678606843672224
24	24.939741836685336	24.88971841770263	25.29757445407815	24.87296529153389
25	24.942631528650022	24.57624019131869	25.52681658960513	24.954311690426163
26	24.63776836643134	25.29123358446644	25.72205930328113	24.348938745821087
27	25.139446297173556	24.88878217588728	24.93874981785703	25.03302170908214
28	24.42052394121589	24.86768148225056	25.694450999084452	25.017343577449104
29	24.681285088725772	24.757324794548268	25.42976591686837	25.131624199857594
30	24.490621801546173	25.0834879406308	25.71363680607378	24.71225345174925
31	25.205002654710633	24.70332133797416	24.864255796118222	25.22742021119698
32	24.786583441435287	24.964029922856795	24.84303832265882	25.406348313049097
33	24.32355104802615	24.65673906387618	25.491950807573893	25.527759080523783
34	25.13657914157333	24.73089735489812	25.493239325118232	24.63928417841032
35	25.507220215541675	24.693320024941436	25.472474458328982	24.326985301187904
36	24.615668694291205	25.11853373897623	25.227908656015885	25.037888910716678
37	25.375735262452785	24.943618326005534	24.94609599308141	24.734550418460273
38	24.65658064344856	24.923941867221995	25.293303875616857	25.126173613712588
39	25.23303343729703	24.7090658428227	24.832182299664392	25.225718420215877
40	25.558349103573907	24.872874556978157	25.27542862805986	24.29334771138808
41	24.567575906127647	25.062457489088008	25.606597162616	24.763369442168347
42	25.624316073561726	25.29827303965667	25.050864954945656	24.026545931835948
43	24.820904819996368	24.6255282599924	25.329285011455955	25.224281908555273
44	24.47724179753196	25.179227591463142	24.950934732428383	25.39259587857651
45	24.71088752397964	25.24628536775862	25.113497735944335	24.929329372317408
46	24.68328146125876	24.838956675216316	25.19036146852635	25.28740039499857
47	24.98426416151725	24.820682230448185	25.424159747518694	24.770893860515876
48	25.323742195500643	25.267110907657965	24.703393629923973	24.70575326691742
49	24.67685305131418	25.347037878627	24.845446329763263	25.130662740295563
50	24.64079141804582	25.659795243586984	24.75694393237316	24.942469405994036
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	19.0
1	22.0
2	25.0
3	104.5
4	184.0
5	201.0
6	218.0
7	222.0
8	226.0
9	315.0
10	404.0
11	649.0
12	894.0
13	1583.0
14	2272.0
15	3081.5
16	3891.0
17	4272.5
18	4654.0
19	4826.0
20	4998.0
21	5678.5
22	6359.0
23	7291.0
24	8223.0
25	9476.0
26	10729.0
27	13570.5
28	16412.0
29	19484.0
30	22556.0
31	25689.5
32	28823.0
33	33476.0
34	38129.0
35	43438.5
36	48748.0
37	56577.5
38	64407.0
39	69244.5
40	74082.0
41	80710.5
42	87339.0
43	89879.0
44	92419.0
45	100860.5
46	109302.0
47	116095.0
48	122888.0
49	126807.0
50	130726.0
51	127711.0
52	124696.0
53	123593.0
54	122490.0
55	122074.0
56	121658.0
57	117615.5
58	113573.0
59	103572.0
60	93571.0
61	82748.0
62	71925.0
63	62325.5
64	52726.0
65	44825.5
66	36925.0
67	31640.0
68	26355.0
69	23583.0
70	20811.0
71	16041.0
72	11271.0
73	9438.0
74	7605.0
75	6006.0
76	4407.0
77	3798.5
78	3190.0
79	2633.5
80	2077.0
81	1714.0
82	1351.0
83	1115.5
84	880.0
85	619.0
86	358.0
87	261.5
88	165.0
89	126.5
90	88.0
91	72.0
92	56.0
93	48.0
94	40.0
95	36.0
96	32.0
97	26.0
98	20.0
99	17.5
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008494512780702354
2	5.898967208821079E-4
3	3.539380325292648E-4
4	0.0
5	0.0
6	1.1797934417642159E-4
7	5.8989672088210796E-5
8	0.0
9	0.0015927211463816916
10	0.0013567624580288484
11	0.0
12	1.1797934417642159E-4
13	5.898967208821079E-4
14	1.769690162646324E-4
15	0.004129277046174756
16	6.488863929703188E-4
17	5.8989672088210796E-5
18	2.9494836044105397E-4
19	0.0
20	0.0010618140975877943
21	0.0
22	1.1797934417642159E-4
23	6.488863929703188E-4
24	3.539380325292648E-4
25	0.001769690162646324
26	0.005604018848380026
27	0.006783812290144241
28	0.003539380325292648
29	0.0026545352439694856
30	0.003362411309028015
31	0.006606843273879609
32	0.003126452620675172
33	0.004306246062439388
34	0.0024185765556166425
35	0.0024185765556166425
36	0.007373709011026349
37	0.004070287374086545
38	0.007491688355202771
39	0.004070287374086545
40	0.0021236281951755886
41	0.003480390653204437
42	0.0021826178672637994
43	0.0014157521301170592
44	0.0012387831138524269
45	0.0014157521301170592
46	9.438347534113727E-4
47	0.0021826178672637994
48	0.0021236281951755886
49	4.7191737670568637E-4
50	0.0015927211463816916
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1695212.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.83651859102932
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.20281857926248	43.87939433460115
2	13.65580932280561	15.52297320902385
3	4.24363204717169	7.235798152276847
4	1.7835214156276116	4.054765923872707
5	0.8754711056418076	2.487936488585979
6	0.5270677105248207	1.797401623678514
7	0.3362340656664747	1.3377261606932969
8	0.23314374969421295	1.0600863251101935
9	0.17232671632439467	0.8815005554492245
>10	0.842439958555235	8.47813513845966
>50	0.06143201099370739	2.4769588244872898
>100	0.05701845471738315	6.673659760793899
>500	0.006891965045572947	2.5812257709101933
>1k	0.0021928979690459378	1.5324377320572344
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.8989672088210796E-5
2	0.0	0.0	0.0	0.0	5.8989672088210796E-5
3	0.0	0.0	0.0	0.0	5.8989672088210796E-5
4	0.0	0.0	0.0	0.0	5.8989672088210796E-5
5	0.0	0.0	0.0	0.0	5.8989672088210796E-5
6	0.0	0.0	0.0	0.0	1.1797934417642159E-4
7	0.0	0.0	0.0	0.0	1.1797934417642159E-4
8	0.0	0.0	0.0	0.0	1.1797934417642159E-4
9	0.0	0.0	0.0	0.0	1.1797934417642159E-4
10	0.0	0.0	0.0	0.0	1.1797934417642159E-4
11	0.0	0.0	0.0	0.0	1.1797934417642159E-4
12	0.0	0.0	0.0	0.0	2.3595868835284318E-4
13	0.0	0.0	0.0	0.0	3.539380325292648E-4
14	0.0	0.0	0.0	0.0	3.539380325292648E-4
15	0.0	0.0	0.0	0.0	4.129277046174756E-4
16	0.0	0.0	0.0	5.8989672088210796E-5	4.129277046174756E-4
17	0.0	0.0	0.0	5.8989672088210796E-5	4.129277046174756E-4
18	0.0	0.0	0.0	5.8989672088210796E-5	4.129277046174756E-4
19	0.0	0.0	0.0	5.8989672088210796E-5	4.129277046174756E-4
20	0.0	0.0	0.0	1.1797934417642159E-4	4.7191737670568637E-4
21	0.0	0.0	0.0	1.769690162646324E-4	4.7191737670568637E-4
22	0.0	0.0	0.0	4.129277046174756E-4	5.309070487938971E-4
23	0.0	0.0	0.0	7.078760650585296E-4	5.309070487938971E-4
24	0.0	0.0	0.0	0.0014157521301170592	5.309070487938971E-4
25	0.0	0.0	0.0	0.0018876695068227455	5.309070487938971E-4
26	0.0	0.0	0.0	0.002300597211440221	5.898967208821079E-4
27	0.0	0.0	0.0	0.0030084732764987508	5.898967208821079E-4
28	0.0	0.0	0.0	0.00595795688090929	5.898967208821079E-4
29	0.0	0.0	0.0	0.013095707203582796	5.898967208821079E-4
30	0.0	0.0	0.0	0.02518858998166601	5.898967208821079E-4
31	0.0	0.0	0.0	0.04961031422618528	6.488863929703187E-4
32	0.0	0.0	0.0	0.07556576994499803	6.488863929703187E-4
33	0.0	0.0	0.0	0.10110829795919331	6.488863929703187E-4
34	0.0	0.0	0.0	0.12641486728503573	7.668657371467404E-4
35	0.0	0.0	0.0	0.15779737283596387	7.668657371467404E-4
36	0.0	0.0	0.0	0.20321942034388618	7.668657371467404E-4
37	0.0	0.0	0.0	0.269759770459388	7.668657371467404E-4
38	0.0	0.0	0.0	0.36756464678164147	8.84845081323162E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCTAC	595	0.0	19.596508	1
GTATCAA	3015	0.0	19.044647	1
TATACTG	255	0.0	18.979706	5
TAGGACG	325	0.0	18.953161	4
TACGACG	430	0.0	18.417938	5
CGACGGT	435	0.0	18.206238	7
GACGGTA	465	0.0	17.504742	8
ATTATAC	240	0.0	17.416037	3
ACGACGG	455	0.0	17.405962	6
ATCGTTT	395	0.0	17.266216	29
CTACGAC	465	0.0	17.031641	4
CGAGCCG	545	0.0	16.954515	15
CGCAATA	535	0.0	16.860693	36
ACGGTAT	480	0.0	16.500864	9
GTCCTAT	510	0.0	16.392046	1
TTAACGG	135	1.0854837E-6	16.297148	35
ATACGAA	560	0.0	16.107037	40
GTATTAC	205	1.05501385E-10	16.097452	1
GTATAAA	315	0.0	16.063385	1
CGTTTAT	440	0.0	16.000837	31
>>END_MODULE