FastQCFastQC Report
Thu 2 Feb 2017
SRR4064055_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064055_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1445689
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36090.2496387535631799No Hit
TATCAACGCAGAGTACTTTTTTTTT25280.17486471848371263No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16490.11406325980207362No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG15740.1088754220306027No Hit
GATTAAGAGGGACGGCCGGGGGCAT14750.10202747617226113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG1850.014.3723551
CGTCCGG551.958335E-413.81956710
GCGTTAT1001.0128133E-813.2944271
CGAACGA1153.765308E-1013.21917316
TCGAACG1550.012.866023
GGACCGA604.0954264E-412.6653066
TCGCGTA701.0894251E-412.2159339
CGACCAT1800.012.14010410
ACGGTAT2350.012.1292949
CGTCTTA1103.805326E-812.0925415
CATTCGC550.003065962612.0921213
CCGACCA1900.012.0016189
ACCGTCG2500.011.7819968
CGAACGT1701.8189894E-1211.7320014
GAATTAA1552.5465852E-1111.6402831
CCGTCGT2500.011.4015379
GATAACG1501.7644197E-1011.40114311
TGAACTA752.077696E-411.3975915
TGAGCCG752.077696E-411.3975915
ACAATTC752.0810384E-411.3956183
Produced by FastQC (version 0.11.5)