##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064055_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1445689
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.229049263015767	32.0	32.0	32.0	32.0	32.0
2	30.743062996259916	32.0	32.0	32.0	32.0	32.0
3	30.76393055491188	32.0	32.0	32.0	32.0	32.0
4	30.8070172768832	32.0	32.0	32.0	32.0	32.0
5	30.669314769635793	32.0	32.0	32.0	32.0	32.0
6	34.35014238885403	36.0	36.0	36.0	32.0	36.0
7	34.29245570797038	36.0	36.0	36.0	32.0	36.0
8	34.22226979661601	36.0	36.0	36.0	32.0	36.0
9	34.40904855746983	36.0	36.0	36.0	32.0	36.0
10	34.02733575478543	36.0	36.0	36.0	32.0	36.0
11	34.396464246459644	36.0	36.0	36.0	32.0	36.0
12	34.16088937523907	36.0	36.0	36.0	32.0	36.0
13	34.27484680315061	36.0	36.0	36.0	32.0	36.0
14	34.145868855611404	36.0	36.0	36.0	32.0	36.0
15	34.07179344935183	36.0	36.0	36.0	32.0	36.0
16	34.07588630749767	36.0	36.0	36.0	32.0	36.0
17	33.997454500933465	36.0	36.0	36.0	32.0	36.0
18	33.98685194395198	36.0	36.0	36.0	32.0	36.0
19	34.00511659146608	36.0	36.0	36.0	32.0	36.0
20	33.978562470904876	36.0	36.0	36.0	32.0	36.0
21	33.98058849448256	36.0	36.0	36.0	32.0	36.0
22	33.958857679625424	36.0	36.0	36.0	32.0	36.0
23	33.885789405605216	36.0	36.0	36.0	32.0	36.0
24	33.87829125074619	36.0	36.0	36.0	32.0	36.0
25	33.45928827016046	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	7.0
4	12.0
5	15.0
6	62.0
7	23.0
8	76.0
9	91.0
10	136.0
11	36.0
12	83.0
13	99.0
14	125.0
15	270.0
16	409.0
17	516.0
18	720.0
19	1059.0
20	1543.0
21	2360.0
22	3629.0
23	5895.0
24	8709.0
25	12526.0
26	17456.0
27	23343.0
28	31086.0
29	41959.0
30	55603.0
31	75188.0
32	104779.0
33	147164.0
34	304946.0
35	605764.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.49563638477316	17.23316624363726	11.26000946626329	26.011187905326295
2	16.93900125001124	19.635207012791344	37.05138466242433	26.37440707477309
3	18.817456073787596	23.858326594787304	28.428617181182613	28.895600150242483
4	12.69674813057283	15.363682270013765	35.74801297704115	36.191556622372254
5	14.745736916744493	36.470409186814706	33.544394867005636	15.239459029435162
6	34.292616547064696	35.446136221029704	16.84039103721993	13.420856194685674
7	30.274258830041166	30.218009478672986	20.931331511398625	18.576400179887226
8	28.367793185529628	32.594937584765695	19.28492346868167	19.752345761023
9	27.833556864910804	13.795427775770838	18.378101933167518	39.99291342615084
10	16.118340000830553	26.437134037457955	31.521919685497156	25.92260627621434
11	37.39906552340586	20.909934644775007	22.36383405323513	19.327165778584
12	24.93247985470191	23.466001824532064	28.771818593338384	22.829699727427645
13	29.76044464132451	19.33096154378582	25.07613721310114	25.832456601788532
14	23.916660898456428	19.474146881705543	25.103066380563437	31.50612583927459
15	25.26001850773913	27.04780796801774	22.091139194947953	25.601034329295175
16	25.89978999478102	25.44101788159139	23.674108446861204	24.98508367676639
17	24.231142193271886	25.64991178545003	25.053831986062864	25.06511403521522
18	25.036827924981793	24.74428128781439	25.967425285001067	24.251465502202755
19	25.86070717300396	24.651788699547346	25.19630042150164	24.291203705947055
20	26.00058565802417	24.174523322134757	24.675309391504083	25.149581628336986
21	27.06560023481068	23.936567887423784	24.234373182847744	24.76345869491779
22	26.259585211378273	24.12804603255147	24.568254458330422	25.04411429773984
23	24.76601966549414	23.976589158986165	25.294660492267347	25.962730683252346
24	25.164965553143414	24.744625293518233	25.024921359266315	25.065487794072038
25	25.102575551471222	24.403601133921065	25.064501278949425	25.429322035658288
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	127.0
1	127.0
2	348.5
3	570.0
4	570.0
5	570.0
6	1478.0
7	2386.0
8	2386.0
9	2386.0
10	2600.5
11	2815.0
12	2815.0
13	2815.0
14	3278.5
15	3742.0
16	3742.0
17	3742.0
18	6320.0
19	8898.0
20	8898.0
21	8898.0
22	14410.0
23	19922.0
24	19922.0
25	19922.0
26	29754.0
27	39586.0
28	39586.0
29	39586.0
30	49817.5
31	60049.0
32	60049.0
33	60049.0
34	74002.5
35	87956.0
36	87956.0
37	87956.0
38	100861.0
39	113766.0
40	113766.0
41	113766.0
42	131422.5
43	149079.0
44	149079.0
45	149079.0
46	166795.0
47	184511.0
48	184511.0
49	184511.0
50	189601.5
51	194692.0
52	194692.0
53	194692.0
54	183617.0
55	172542.0
56	172542.0
57	172542.0
58	159238.5
59	145935.0
60	145935.0
61	145935.0
62	128635.5
63	111336.0
64	111336.0
65	111336.0
66	91473.5
67	71611.0
68	71611.0
69	71611.0
70	53983.0
71	36355.0
72	36355.0
73	36355.0
74	27875.0
75	19395.0
76	19395.0
77	19395.0
78	15365.0
79	11335.0
80	11335.0
81	11335.0
82	7888.5
83	4442.0
84	4442.0
85	4442.0
86	3371.5
87	2301.0
88	2301.0
89	2301.0
90	1655.0
91	1009.0
92	1009.0
93	1009.0
94	735.0
95	461.0
96	461.0
97	461.0
98	664.5
99	868.0
100	868.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03852834184945725
2	0.007055459369200429
3	0.0017984504274432469
4	0.004081099046890445
5	0.009614792669792743
6	0.013834234057255744
7	0.023449026727048485
8	0.036591549081441445
9	0.04862733271125394
10	0.060109746978776205
11	0.05644367495360343
12	0.0646750442176706
13	0.06426001719595294
14	0.06841028741312966
15	0.061423999214215506
16	0.06661183698568642
17	0.06336079198223131
18	0.07837093593435379
19	0.07553491795261637
20	0.08023855753208331
21	0.07754088189091846
22	0.07927016114807542
23	0.08508053945212282
24	0.07920098997778914
25	0.07913181880750286
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1445689.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.95496491707541
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.41715396314433	40.46667707181058
2	13.942795815101224	14.766805264692625
3	4.413909948233129	7.012153393674464
4	1.8622007529551061	3.944511021651563
5	0.9354213081191517	2.476760127706724
6	0.55273124852183	1.7561918324426877
7	0.3716614052260004	1.377692167234166
8	0.2481238102934199	1.05115101353433
9	0.17270415360690167	0.8230988155758012
>10	0.9078778623455335	8.76532488554553
>50	0.08223652822353157	3.022859046947125
>100	0.08086369291642287	8.915000935804922
>500	0.008780928276700887	3.061255114512378
>1k	0.003538583036879462	2.5605193088671174
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3609	0.2496387535631799	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2528	0.17486471848371263	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1649	0.11406325980207362	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1574	0.1088754220306027	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1475	0.10202747617226113	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3834234057255744E-4
2	0.0	0.0	0.0	0.0	1.3834234057255744E-4
3	0.0	0.0	0.0	0.0	1.3834234057255744E-4
4	0.0	0.0	0.0	0.0	1.3834234057255744E-4
5	0.0	6.917117028627872E-5	0.0	6.917117028627872E-5	1.3834234057255744E-4
6	0.0	6.917117028627872E-5	0.0	6.917117028627872E-5	1.3834234057255744E-4
7	0.0	6.917117028627872E-5	0.0	6.917117028627872E-5	1.3834234057255744E-4
8	0.0	6.917117028627872E-5	0.0	6.917117028627872E-5	2.0751351085883618E-4
9	0.0	6.917117028627872E-5	0.0	6.917117028627872E-5	2.0751351085883618E-4
10	0.0	6.917117028627872E-5	0.0	6.917117028627872E-5	2.766846811451149E-4
11	0.0	6.917117028627872E-5	0.0	6.917117028627872E-5	2.766846811451149E-4
12	0.0	6.917117028627872E-5	0.0	1.3834234057255744E-4	5.533693622902298E-4
13	0.0	1.3834234057255744E-4	0.0	1.3834234057255744E-4	5.533693622902298E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAG	185	0.0	14.372355	1
CGTCCGG	55	1.958335E-4	13.819567	10
GCGTTAT	100	1.0128133E-8	13.294427	1
CGAACGA	115	3.765308E-10	13.219173	16
TCGAACG	155	0.0	12.86602	3
GGACCGA	60	4.0954264E-4	12.665306	6
TCGCGTA	70	1.0894251E-4	12.215933	9
CGACCAT	180	0.0	12.140104	10
ACGGTAT	235	0.0	12.129294	9
CGTCTTA	110	3.805326E-8	12.09254	15
CATTCGC	55	0.0030659626	12.09212	13
CCGACCA	190	0.0	12.001618	9
ACCGTCG	250	0.0	11.781996	8
CGAACGT	170	1.8189894E-12	11.732001	4
GAATTAA	155	2.5465852E-11	11.640283	1
CCGTCGT	250	0.0	11.401537	9
GATAACG	150	1.7644197E-10	11.401143	11
TGAACTA	75	2.077696E-4	11.397591	5
TGAGCCG	75	2.077696E-4	11.397591	5
ACAATTC	75	2.0810384E-4	11.395618	3
>>END_MODULE