FastQCFastQC Report
Thu 2 Feb 2017
SRR4064055_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064055_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1445689
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15150.10479432298371226No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA14550.10064405276653554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29450.020.543261
TAACGGC1502.1827873E-1120.5354336
GTATTAG3800.020.2631261
ATACCGT3500.019.4850086
CGTCGTA3400.019.41240510
TTAACGG1605.2750693E-1119.25196835
ACCGTCG3650.018.6842568
TACCGTC3850.018.2850537
CCGTCGT3800.017.9479629
TAGGACG2750.017.5993634
GCATTCG6150.017.52721422
TTCGTAT6250.016.89597325
GACGGCC6300.016.76245911
GTCGTAG3950.016.70941211
AACGCAG36250.016.689056
GCATATA2151.2732926E-1116.3720681
CATATAG1753.6307028E-916.3417022
ATTCGTA6350.016.28231224
TATAGGG1906.166374E-1016.209944
CGTATTG6450.016.03153627
Produced by FastQC (version 0.11.5)