##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064055_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1445689
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.281739710269637	32.0	32.0	32.0	32.0	32.0
2	31.403770797176985	32.0	32.0	32.0	32.0	32.0
3	31.477795708482255	32.0	32.0	32.0	32.0	32.0
4	31.557432476832847	32.0	32.0	32.0	32.0	32.0
5	31.519572328488355	32.0	32.0	32.0	32.0	32.0
6	35.09615899408517	36.0	36.0	36.0	36.0	36.0
7	35.11116844632559	36.0	36.0	36.0	36.0	36.0
8	35.0585699967282	36.0	36.0	36.0	36.0	36.0
9	35.18139447695874	36.0	36.0	36.0	36.0	36.0
10	35.021960463142484	36.0	36.0	36.0	36.0	36.0
11	35.164879860052885	36.0	36.0	36.0	36.0	36.0
12	35.069158719475624	36.0	36.0	36.0	36.0	36.0
13	35.12053491449406	36.0	36.0	36.0	36.0	36.0
14	35.05934955581733	36.0	36.0	36.0	36.0	36.0
15	35.03231538733434	36.0	36.0	36.0	36.0	36.0
16	35.046698148771966	36.0	36.0	36.0	36.0	36.0
17	35.00595771289676	36.0	36.0	36.0	36.0	36.0
18	35.00003942756706	36.0	36.0	36.0	36.0	36.0
19	35.00282080032427	36.0	36.0	36.0	36.0	36.0
20	34.99667286670923	36.0	36.0	36.0	36.0	36.0
21	34.991794915780645	36.0	36.0	36.0	36.0	36.0
22	34.972352283236575	36.0	36.0	36.0	36.0	36.0
23	34.92396497448621	36.0	36.0	36.0	32.0	36.0
24	34.89906335318315	36.0	36.0	36.0	32.0	36.0
25	34.864867893440426	36.0	36.0	36.0	32.0	36.0
26	34.810477910532626	36.0	36.0	36.0	32.0	36.0
27	34.80082299858407	36.0	36.0	36.0	32.0	36.0
28	34.783663014659446	36.0	36.0	36.0	32.0	36.0
29	34.74688539512994	36.0	36.0	36.0	32.0	36.0
30	34.7366570541797	36.0	36.0	36.0	32.0	36.0
31	34.7316545951446	36.0	36.0	36.0	32.0	36.0
32	34.678556729697746	36.0	36.0	36.0	32.0	36.0
33	34.647429703068916	36.0	36.0	36.0	32.0	36.0
34	34.63660510663082	36.0	36.0	36.0	32.0	36.0
35	34.58490864909396	36.0	36.0	36.0	32.0	36.0
36	34.57325607374754	36.0	36.0	36.0	32.0	36.0
37	34.564388329716834	36.0	36.0	36.0	32.0	36.0
38	34.51993132686214	36.0	36.0	36.0	32.0	36.0
39	34.52053795802555	36.0	36.0	36.0	32.0	36.0
40	34.504987587233494	36.0	36.0	36.0	32.0	36.0
41	34.48171218014386	36.0	36.0	36.0	32.0	36.0
42	34.4172799267339	36.0	36.0	36.0	32.0	36.0
43	34.41325139777642	36.0	36.0	36.0	32.0	36.0
44	34.34490751468677	36.0	36.0	36.0	32.0	36.0
45	34.31311021941787	36.0	36.0	36.0	32.0	36.0
46	34.285604303553534	36.0	36.0	36.0	32.0	36.0
47	34.252216071368046	36.0	36.0	36.0	32.0	36.0
48	34.20140016282893	36.0	36.0	36.0	32.0	36.0
49	34.17322397832452	36.0	36.0	36.0	32.0	36.0
50	33.58141135472429	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	2.0
17	1.0
18	3.0
19	0.0
20	14.0
21	58.0
22	187.0
23	501.0
24	1305.0
25	2810.0
26	5652.0
27	10235.0
28	17346.0
29	26077.0
30	36815.0
31	51442.0
32	74295.0
33	119959.0
34	246321.0
35	852665.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.514985320445945	18.050801495349862	11.860830633088627	26.573382551115564
2	16.077809642784512	20.362776876242496	36.7767600343644	26.782653446608588
3	18.01695115734224	24.514641176523483	28.701243633631996	28.767164032502286
4	12.234374059704404	16.046743110032654	35.9056477568827	35.813235073380234
5	14.2891728442286	36.9380966445757	33.31110633061468	15.461624180581024
6	34.30293002565562	35.81037942514528	16.603732343169717	13.282958206029383
7	30.11260390388791	30.43632542867163	20.967194143683937	18.483876523756525
8	28.21332942285651	32.91517055189602	19.206136312858433	19.66536371238904
9	27.760315646469312	13.687236626943392	18.314232590055255	40.23821513653204
10	15.90459002944641	26.771816941128616	31.59869044053409	25.72490258889088
11	37.439821040224444	20.815487159055067	22.450418070842396	19.294273729878093
12	24.907051111340145	23.585949102399066	28.749791621561236	22.757208164699552
13	29.829886399558408	19.357313266204645	25.08651631999037	25.726284014246577
14	23.816151075578603	19.475100765241717	25.07707408311758	31.631674076062104
15	25.217190397759854	27.142798838699772	22.114994178972218	25.525016584568156
16	25.84903245888599	25.51403323706919	23.71667214277068	24.920262161274145
17	24.269327635473466	25.66630859057515	24.953084653753333	25.11127912019805
18	24.97508101695736	24.733949650165837	26.057889512583998	24.2330798202928
19	25.898585380396476	24.554105343542076	25.363269693550965	24.184039582510486
20	26.129316755148977	23.974406419146767	24.810832310166532	25.085444515537723
21	27.135435076285425	23.82594043393842	24.36070275142164	24.677921738354513
22	26.207729468599034	24.077670977416982	24.75665565460874	24.957943899375245
23	24.720252172511564	23.93342357940259	25.481418048429905	25.86490619965594
24	25.132238350678055	24.65461009832709	25.141438141780544	25.07171340921432
25	25.107839420945886	24.17567518960093	25.301798199863317	25.414687189589863
26	24.910712741635912	24.9546386831902	25.564828809883682	24.569819765290205
27	25.50461990338913	24.59820986958293	24.756622368813353	25.140547858214585
28	24.809045719732655	24.39427960327441	25.519460665701	25.27721401129193
29	24.83635445524693	24.56471288101241	25.300365578232704	25.298567085507955
30	24.656619821973564	24.756368858378504	25.666699870229408	24.920311449418524
31	25.389688145718775	24.493507445805452	24.666448530312078	25.4503558781637
32	25.06526512096356	24.603601721588056	24.547709598918683	25.783423558529705
33	24.751073941104448	24.300399139463618	25.17553143655619	25.772995482875743
34	25.485892397756288	24.2991507561708	25.39264643524421	24.822310410828706
35	25.869214087829306	24.32678838857396	25.19131598698159	24.612681536615145
36	24.71177459410475	24.93106525453627	24.962609904743456	25.394550246615523
37	25.625119323982943	24.817589946072477	24.68173353882422	24.87555719112036
38	24.98241172136846	24.560293034211536	25.18018906102458	25.27710618339542
39	25.537016118265637	24.450497950024452	24.602888573608546	25.409597358101365
40	25.786049973506653	24.569638763570058	25.067755176578498	24.576556086344787
41	24.774649202599548	25.05355777910745	25.26439938158664	24.90739363670636
42	25.857119536044536	25.143740972956223	24.858265036772234	24.140874454227006
43	25.069864283441472	24.261928807603447	25.1833764508944	25.484830458060674
44	24.89292117670496	24.865044713017685	24.706363304336275	25.535670805941074
45	24.89636228687085	25.058087615284634	24.802080155721153	25.243469942123358
46	24.87154762629421	24.823403925648417	24.92557094555335	25.379477502504027
47	25.220609343169702	24.53393098630033	25.242122083069614	25.003337587460354
48	25.82118315673555	25.101925905020412	24.273168342720062	24.803722595523976
49	25.02362213438954	25.21488187549371	24.438637096227513	25.322858893889233
50	24.927507359939792	25.451003829382206	24.463844987493637	25.15764382318436
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	21.0
1	24.0
2	27.0
3	79.0
4	131.0
5	151.0
6	171.0
7	187.5
8	204.0
9	289.5
10	375.0
11	577.5
12	780.0
13	1424.5
14	2069.0
15	2818.5
16	3568.0
17	3993.5
18	4419.0
19	4633.0
20	4847.0
21	5260.5
22	5674.0
23	6259.0
24	6844.0
25	7723.0
26	8602.0
27	10593.5
28	12585.0
29	14760.0
30	16935.0
31	19416.0
32	21897.0
33	25424.0
34	28951.0
35	33544.5
36	38138.0
37	44817.0
38	51496.0
39	55761.5
40	60027.0
41	65190.5
42	70354.0
43	72727.5
44	75101.0
45	82968.5
46	90836.0
47	97307.0
48	103778.0
49	108155.0
50	112532.0
51	110225.5
52	107919.0
53	106890.0
54	105861.0
55	106967.5
56	108074.0
57	105131.0
58	102188.0
59	93309.0
60	84430.0
61	74469.5
62	64509.0
63	55954.0
64	47399.0
65	40163.0
66	32927.0
67	28201.5
68	23476.0
69	20981.5
70	18487.0
71	14309.5
72	10132.0
73	8741.0
74	7350.0
75	5758.0
76	4166.0
77	3657.0
78	3148.0
79	2674.5
80	2201.0
81	1738.0
82	1275.0
83	1097.5
84	920.0
85	668.0
86	416.0
87	293.5
88	171.0
89	137.0
90	103.0
91	80.5
92	58.0
93	53.0
94	48.0
95	42.0
96	36.0
97	25.0
98	14.0
99	16.5
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0097531350103653
2	4.8419819200395107E-4
3	4.150270217176723E-4
4	0.0
5	0.0
6	1.3834234057255744E-4
7	1.3834234057255744E-4
8	0.0
9	0.0014525945760118532
10	8.300540434353446E-4
11	6.917117028627872E-5
12	1.3834234057255744E-4
13	5.533693622902298E-4
14	1.3834234057255744E-4
15	0.004703639579466953
16	9.683963840079021E-4
17	0.0
18	2.766846811451149E-4
19	0.0
20	9.683963840079021E-4
21	0.0
22	6.917117028627872E-5
23	8.300540434353446E-4
24	2.766846811451149E-4
25	0.0014525945760118532
26	0.0051186666011846255
27	0.006640432347482757
28	0.00380441436574533
29	0.0023518197897334763
30	0.004219441387463002
31	0.00760882873149066
32	0.0032510450034551
33	0.00546452245261602
34	0.003458558514313936
35	0.0030435314925962636
36	0.008231369264067168
37	0.003112702662882543
38	0.008577225115498563
39	0.0042886125577492815
40	0.0029743603223099852
41	0.003735243195459051
42	0.002282648619447198
43	0.0020059639383020827
44	0.001729279257156968
45	0.0019367927680158043
46	0.0011759098948667382
47	0.0023518197897334763
48	0.0021443062788746407
49	7.608828731490659E-4
50	0.001729279257156968
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1445689.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.19883309009117
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.29822055299213	43.4406979501866
2	13.770627329590255	15.477863736829814
3	4.195990330938017	7.07429280768066
4	1.7250423142731501	3.877814603727255
5	0.8545348628659718	2.4011931063934346
6	0.49026608180509823	1.6531429020658621
7	0.3338988389253091	1.313530758241708
8	0.21387132364908626	0.9615455056409493
9	0.14937129450649972	0.7555043203579468
>10	0.8193301109739285	8.215862864047477
>50	0.07131826608061012	2.7572997545297424
>100	0.06837865443818533	7.961440486711855
>500	0.007295301334343383	2.832248702902812
>1k	0.0018547376273754367	1.2775625006837479
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1515	0.10479432298371226	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1455	0.10064405276653554	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.917117028627872E-5
2	0.0	0.0	0.0	0.0	6.917117028627872E-5
3	0.0	0.0	0.0	0.0	6.917117028627872E-5
4	0.0	0.0	0.0	0.0	6.917117028627872E-5
5	0.0	0.0	0.0	6.917117028627872E-5	6.917117028627872E-5
6	0.0	0.0	0.0	6.917117028627872E-5	2.0751351085883618E-4
7	0.0	0.0	0.0	6.917117028627872E-5	2.0751351085883618E-4
8	0.0	0.0	0.0	6.917117028627872E-5	2.0751351085883618E-4
9	0.0	0.0	0.0	6.917117028627872E-5	2.0751351085883618E-4
10	0.0	0.0	0.0	6.917117028627872E-5	2.0751351085883618E-4
11	0.0	0.0	0.0	6.917117028627872E-5	2.0751351085883618E-4
12	0.0	0.0	0.0	1.3834234057255744E-4	7.608828731490659E-4
13	0.0	0.0	0.0	1.3834234057255744E-4	0.0010375675542941807
14	0.0	0.0	0.0	1.3834234057255744E-4	0.0010375675542941807
15	0.0	0.0	0.0	2.0751351085883618E-4	0.0010375675542941807
16	0.0	0.0	0.0	2.0751351085883618E-4	0.0011759098948667384
17	0.0	0.0	0.0	2.766846811451149E-4	0.0011759098948667384
18	0.0	0.0	0.0	2.766846811451149E-4	0.0011759098948667384
19	0.0	0.0	0.0	2.766846811451149E-4	0.0011759098948667384
20	0.0	1.3834234057255744E-4	0.0	3.458558514313936E-4	0.001245081065153017
21	0.0	1.3834234057255744E-4	0.0	4.1502702171767236E-4	0.001245081065153017
22	0.0	1.3834234057255744E-4	0.0	5.533693622902298E-4	0.0013142522354392958
23	0.0	1.3834234057255744E-4	0.0	0.0011759098948667384	0.0013142522354392958
24	0.0	1.3834234057255744E-4	0.0	0.0016601080868706894	0.0013142522354392958
25	0.0	1.3834234057255744E-4	0.0	0.002005963938302083	0.0013142522354392958
26	0.0	1.3834234057255744E-4	0.0	0.002420990960019755	0.0013834234057255744
27	0.0	1.3834234057255744E-4	0.0	0.0031127026628825424	0.0013834234057255744
28	0.0	1.3834234057255744E-4	0.0	0.006017891814906248	0.0014525945760118532
29	0.0	1.3834234057255744E-4	0.0	0.01293500884353412	0.0014525945760118532
30	0.0	1.3834234057255744E-4	0.0	0.02704592758193498	0.0014525945760118532
31	0.0	1.3834234057255744E-4	0.0	0.0529851164392895	0.0014525945760118532
32	0.0	1.3834234057255744E-4	0.0	0.07837093593435379	0.0014525945760118532
33	0.0	1.3834234057255744E-4	0.0	0.10507100766485739	0.0014525945760118532
34	0.0	1.3834234057255744E-4	0.0	0.13384621450394932	0.0015909369165844106
35	0.0	1.3834234057255744E-4	0.0	0.16725588975222194	0.0015909369165844106
36	0.0	1.3834234057255744E-4	0.0	0.20972698830799708	0.0015909369165844106
37	0.0	1.3834234057255744E-4	0.0	0.27993572614857	0.0015909369165844106
38	0.0	1.3834234057255744E-4	0.0	0.3693048781584421	0.001729279257156968
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2945	0.0	20.54326	1
TAACGGC	150	2.1827873E-11	20.53543	36
GTATTAG	380	0.0	20.263126	1
ATACCGT	350	0.0	19.485008	6
CGTCGTA	340	0.0	19.412405	10
TTAACGG	160	5.2750693E-11	19.251968	35
ACCGTCG	365	0.0	18.684256	8
TACCGTC	385	0.0	18.285053	7
CCGTCGT	380	0.0	17.947962	9
TAGGACG	275	0.0	17.599363	4
GCATTCG	615	0.0	17.527214	22
TTCGTAT	625	0.0	16.895973	25
GACGGCC	630	0.0	16.762459	11
GTCGTAG	395	0.0	16.709412	11
AACGCAG	3625	0.0	16.68905	6
GCATATA	215	1.2732926E-11	16.372068	1
CATATAG	175	3.6307028E-9	16.341702	2
ATTCGTA	635	0.0	16.282312	24
TATAGGG	190	6.166374E-10	16.20994	4
CGTATTG	645	0.0	16.031536	27
>>END_MODULE