FastQCFastQC Report
Thu 2 Feb 2017
SRR4064054_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064054_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1041343
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31320.30076545384181774No Hit
TATCAACGCAGAGTACTTTTTTTTT21340.20492767512721552No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16370.1572008454466972No Hit
GGTATCAACGCAGAGTACTTTTTTT11100.10659312061443732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGG653.3654687E-614.61708514
GACTCGC551.958835E-413.8184627
ACCGTCG903.6332494E-813.72258
GCATCGC1051.366061E-913.57300814
CTAGGAC1750.013.5664863
TCCAACG1102.730303E-912.95792218
CGCATCG1102.737579E-912.95605313
CGCGGCG751.4749745E-512.66875116
TGACTCG751.4778934E-512.6663146
ATAACGC1457.2759576E-1212.4437433
GGCGAGG2750.012.44020319
ATTACCG1301.9645086E-1012.4251215
CCGACCA1155.313268E-912.3915569
CTATACA1001.4447687E-712.3472824
TCGCACG701.0875834E-412.21746818
AACGCCG1405.2750693E-1112.2121855
CGACCAT1103.8044163E-812.09173610
GTTATAT550.003072332612.0882491
TAACGCC1501.4551915E-1112.0306864
TACCGTC951.0384101E-612.0002427
Produced by FastQC (version 0.11.5)