##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064054_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1041343
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.157390024228327	32.0	32.0	32.0	32.0	32.0
2	30.71054301992715	32.0	32.0	32.0	32.0	32.0
3	30.73064974748954	32.0	32.0	32.0	32.0	32.0
4	30.7602499848753	32.0	32.0	32.0	32.0	32.0
5	30.664824174167396	32.0	32.0	32.0	32.0	32.0
6	34.30116685856629	36.0	36.0	36.0	32.0	36.0
7	34.2305445948165	36.0	36.0	36.0	32.0	36.0
8	34.173416443957464	36.0	36.0	36.0	32.0	36.0
9	34.341629030972506	36.0	36.0	36.0	32.0	36.0
10	33.99225327293696	36.0	36.0	36.0	32.0	36.0
11	34.333160159524766	36.0	36.0	36.0	32.0	36.0
12	34.11013278045754	36.0	36.0	36.0	32.0	36.0
13	34.21453065896635	36.0	36.0	36.0	32.0	36.0
14	34.09721868779067	36.0	36.0	36.0	32.0	36.0
15	34.03814593270421	36.0	36.0	36.0	32.0	36.0
16	34.036979170167754	36.0	36.0	36.0	32.0	36.0
17	33.95484869058514	36.0	36.0	36.0	32.0	36.0
18	33.95518767591466	36.0	36.0	36.0	32.0	36.0
19	33.9721811161164	36.0	36.0	36.0	32.0	36.0
20	33.941495741556814	36.0	36.0	36.0	32.0	36.0
21	33.9295928430882	36.0	36.0	36.0	32.0	36.0
22	33.908490286101696	36.0	36.0	36.0	32.0	36.0
23	33.85673788559581	36.0	36.0	36.0	32.0	36.0
24	33.83980494419226	36.0	36.0	36.0	32.0	36.0
25	33.42990061872025	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	4.0
4	11.0
5	13.0
6	44.0
7	21.0
8	56.0
9	74.0
10	87.0
11	25.0
12	60.0
13	61.0
14	100.0
15	198.0
16	327.0
17	415.0
18	542.0
19	735.0
20	1125.0
21	1646.0
22	2692.0
23	4223.0
24	6261.0
25	9342.0
26	13454.0
27	17504.0
28	23650.0
29	31353.0
30	41040.0
31	55482.0
32	76635.0
33	106436.0
34	218924.0
35	428801.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.94810875306337	17.52562859835512	11.741649510484404	25.7846131380971
2	16.092535753934563	20.022971638861254	38.26612144237307	25.61837116483111
3	18.602446894326434	24.32623977259632	28.898033265470747	28.1732800676065
4	12.569924949221884	15.899144814869945	36.320255067007906	35.210675168900266
5	14.238463385354141	36.992076830732294	34.0439855942377	14.725474189675868
6	33.407030137254694	35.91413009758904	17.274633284767717	13.40420648038855
7	29.53958667112346	30.814958678638526	21.244673943529392	18.400780706708623
8	27.672784387563464	33.54522006023257	19.684043171480308	19.097952380723658
9	27.52948410620523	14.172931789030713	18.84878676806157	39.44879733670249
10	15.870645762290064	27.207974333622552	32.19164459100697	24.72973531308042
11	36.46618202921276	21.159459558138238	23.147203342115226	19.227155070533776
12	24.69277328979774	23.830165055382004	29.498088271045592	21.978973383774665
13	29.695220268169265	20.08944100333818	25.243637369965583	24.97170135852697
14	23.27040882512624	20.033536248456514	25.751706841298592	30.94434808511865
15	24.592562983262436	28.17145553096771	22.56155155167659	24.67442993409326
16	24.86972197424901	25.96133795988888	24.890766331718652	24.27817373414346
17	23.505728405453684	26.262454465350427	25.896351558723964	24.335465570471925
18	24.058489142604525	25.397607961289143	27.19745131110438	23.346451585001944
19	25.382050222474223	24.872810094467454	25.804318786818765	23.94082089623956
20	25.611787217316007	24.848705018755098	25.578246198764653	23.96126156516424
21	26.215137298165835	24.427579950450806	24.86771825766734	24.489564493716017
22	25.50041517064282	25.125992757578786	25.21825290042824	24.15533917135015
23	24.13484891151513	25.027030715542686	25.863949723349638	24.974170649592544
24	24.666325816694474	25.214312623494045	25.884159702307276	24.23520185750421
25	24.551794790968913	25.033756543483715	25.985090419484518	24.429358246062858
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	275.0
1	275.0
2	405.0
3	535.0
4	535.0
5	535.0
6	1390.5
7	2246.0
8	2246.0
9	2246.0
10	2347.0
11	2448.0
12	2448.0
13	2448.0
14	2826.5
15	3205.0
16	3205.0
17	3205.0
18	5507.0
19	7809.0
20	7809.0
21	7809.0
22	12409.5
23	17010.0
24	17010.0
25	17010.0
26	25713.0
27	34416.0
28	34416.0
29	34416.0
30	43704.0
31	52992.0
32	52992.0
33	52992.0
34	62328.0
35	71664.0
36	71664.0
37	71664.0
38	80234.0
39	88804.0
40	88804.0
41	88804.0
42	99997.0
43	111190.0
44	111190.0
45	111190.0
46	122802.5
47	134415.0
48	134415.0
49	134415.0
50	136491.0
51	138567.0
52	138567.0
53	138567.0
54	128085.5
55	117604.0
56	117604.0
57	117604.0
58	106980.0
59	96356.0
60	96356.0
61	96356.0
62	83456.0
63	70556.0
64	70556.0
65	70556.0
66	57532.0
67	44508.0
68	44508.0
69	44508.0
70	33644.0
71	22780.0
72	22780.0
73	22780.0
74	17460.0
75	12140.0
76	12140.0
77	12140.0
78	9399.0
79	6658.0
80	6658.0
81	6658.0
82	4596.0
83	2534.0
84	2534.0
85	2534.0
86	1915.0
87	1296.0
88	1296.0
89	1296.0
90	940.0
91	584.0
92	584.0
93	584.0
94	410.5
95	237.0
96	237.0
97	237.0
98	375.5
99	514.0
100	514.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03994841277081614
2	0.005665760465091713
3	0.0022086862830018542
4	0.0046094322427864785
5	0.008930774970398802
6	0.013444177374793897
7	0.022567012021975467
8	0.03629927891194352
9	0.05070375467065126
10	0.05800202238839652
11	0.05425685869113251
12	0.06357175301509685
13	0.06309160382313993
14	0.06510823042935901
15	0.06049879818657253
16	0.06568440945970731
17	0.06261145463118299
18	0.07759210942023906
19	0.07423106507654058
20	0.07932064651128398
21	0.07307870701584396
22	0.07749607958184768
23	0.08258566101659108
24	0.07749607958184768
25	0.07720799006667352
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1041343.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.0326362690047
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.11079366905975	46.70118707617883
2	12.806189885897307	15.119662990519652
3	3.6890150789971736	6.533168560479415
4	1.5099988460748888	3.56556850587823
5	0.7720166697104575	2.2787089628312884
6	0.4829809635805633	1.7106983728742882
7	0.31057132500496865	1.283369084522081
8	0.1983480023122041	0.9367204380144036
9	0.1633055061199111	0.8676319089152191
>10	0.8172118527640517	8.577240966046316
>50	0.0729009345150991	3.0184528437227294
>100	0.06209206213305127	7.062758530617994
>500	0.0035948030097731065	1.3745465700540422
>1k	9.80400820847211E-4	0.9702851893455166
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3132	0.30076545384181774	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2134	0.20492767512721552	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1637	0.1572008454466972	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1110	0.10659312061443732	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	9.602983839138497E-5	9.602983839138497E-5
10	0.0	0.0	0.0	9.602983839138497E-5	9.602983839138497E-5
11	0.0	0.0	9.602983839138497E-5	9.602983839138497E-5	9.602983839138497E-5
12	0.0	9.602983839138497E-5	9.602983839138497E-5	9.602983839138497E-5	1.9205967678276995E-4
13	0.0	9.602983839138497E-5	9.602983839138497E-5	9.602983839138497E-5	1.9205967678276995E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGCGG	65	3.3654687E-6	14.617085	14
GACTCGC	55	1.958835E-4	13.818462	7
ACCGTCG	90	3.6332494E-8	13.7225	8
GCATCGC	105	1.366061E-9	13.573008	14
CTAGGAC	175	0.0	13.566486	3
TCCAACG	110	2.730303E-9	12.957922	18
CGCATCG	110	2.737579E-9	12.956053	13
CGCGGCG	75	1.4749745E-5	12.668751	16
TGACTCG	75	1.4778934E-5	12.666314	6
ATAACGC	145	7.2759576E-12	12.443743	3
GGCGAGG	275	0.0	12.440203	19
ATTACCG	130	1.9645086E-10	12.42512	15
CCGACCA	115	5.313268E-9	12.391556	9
CTATACA	100	1.4447687E-7	12.347282	4
TCGCACG	70	1.0875834E-4	12.217468	18
AACGCCG	140	5.2750693E-11	12.212185	5
CGACCAT	110	3.8044163E-8	12.091736	10
GTTATAT	55	0.0030723326	12.088249	1
TAACGCC	150	1.4551915E-11	12.030686	4
TACCGTC	95	1.0384101E-6	12.000242	7
>>END_MODULE