FastQCFastQC Report
Thu 2 Feb 2017
SRR4064054_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064054_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1041343
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG1205.984475E-1022.0005761
GTATCAA22550.020.3907781
TTAACGG1101.2642158E-720.00148635
TATAGGA2000.018.6986942
CGATTAA600.0074103118.33381341
ACGATTA600.007412363318.33293240
CCGAAAT600.007414416418.33205212
GTCCTAT3050.018.033261
GGCGTTA2003.6379788E-1217.60130542
GTCCTAA3400.017.4710461
GATATAC2700.017.1115591
CTAGATA1802.8558134E-1017.1099173
GTATTAG2450.017.0616721
GTATATA1551.4379111E-817.0327031
AACGCAG26850.016.8777776
ATACCGT1854.2200554E-1016.6474866
GTCCTAC6450.016.0314271
TAGGACG6750.015.9692544
TAGGACA2800.015.7131884
TACCGTC2101.5097612E-1015.7131887
Produced by FastQC (version 0.11.5)