##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064054_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1041343
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.278199402118226	32.0	32.0	32.0	32.0	32.0
2	31.37764502186119	32.0	32.0	32.0	32.0	32.0
3	31.467899625771718	32.0	32.0	32.0	32.0	32.0
4	31.54779549101497	32.0	32.0	32.0	32.0	32.0
5	31.498237372316325	32.0	32.0	32.0	32.0	32.0
6	35.07086713983769	36.0	36.0	36.0	36.0	36.0
7	35.090679055796215	36.0	36.0	36.0	36.0	36.0
8	35.041166071121616	36.0	36.0	36.0	36.0	36.0
9	35.14881263906321	36.0	36.0	36.0	36.0	36.0
10	34.99720073021089	36.0	36.0	36.0	36.0	36.0
11	35.149042150376964	36.0	36.0	36.0	36.0	36.0
12	35.05388810411171	36.0	36.0	36.0	36.0	36.0
13	35.08599856147302	36.0	36.0	36.0	36.0	36.0
14	35.03717026954615	36.0	36.0	36.0	36.0	36.0
15	35.01023005868383	36.0	36.0	36.0	36.0	36.0
16	35.0259683888978	36.0	36.0	36.0	36.0	36.0
17	34.990181909322864	36.0	36.0	36.0	36.0	36.0
18	34.98339259974859	36.0	36.0	36.0	36.0	36.0
19	34.97860743290155	36.0	36.0	36.0	36.0	36.0
20	34.96722981764894	36.0	36.0	36.0	36.0	36.0
21	34.96905822577191	36.0	36.0	36.0	36.0	36.0
22	34.945408957471265	36.0	36.0	36.0	36.0	36.0
23	34.89885081092397	36.0	36.0	36.0	32.0	36.0
24	34.87352390134663	36.0	36.0	36.0	32.0	36.0
25	34.849954337811845	36.0	36.0	36.0	32.0	36.0
26	34.78623181795047	36.0	36.0	36.0	32.0	36.0
27	34.7718791983045	36.0	36.0	36.0	32.0	36.0
28	34.74631125383279	36.0	36.0	36.0	32.0	36.0
29	34.72059158221643	36.0	36.0	36.0	32.0	36.0
30	34.692643058051	36.0	36.0	36.0	32.0	36.0
31	34.69302525680779	36.0	36.0	36.0	32.0	36.0
32	34.64863834490653	36.0	36.0	36.0	32.0	36.0
33	34.618710645771856	36.0	36.0	36.0	32.0	36.0
34	34.608708177805006	36.0	36.0	36.0	32.0	36.0
35	34.56624186267157	36.0	36.0	36.0	32.0	36.0
36	34.53721588371939	36.0	36.0	36.0	32.0	36.0
37	34.51781017397726	36.0	36.0	36.0	32.0	36.0
38	34.48408257413744	36.0	36.0	36.0	32.0	36.0
39	34.454035798003154	36.0	36.0	36.0	32.0	36.0
40	34.45216513675129	36.0	36.0	36.0	32.0	36.0
41	34.429211124480595	36.0	36.0	36.0	32.0	36.0
42	34.36970911601653	36.0	36.0	36.0	32.0	36.0
43	34.35806261721642	36.0	36.0	36.0	32.0	36.0
44	34.28655975984858	36.0	36.0	36.0	32.0	36.0
45	34.244177950972926	36.0	36.0	36.0	32.0	36.0
46	34.21242952610235	36.0	36.0	36.0	32.0	36.0
47	34.16503976115459	36.0	36.0	36.0	32.0	36.0
48	34.11775754962582	36.0	36.0	36.0	32.0	36.0
49	34.09715050660541	36.0	36.0	36.0	32.0	36.0
50	33.498575397347466	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	6.0
20	10.0
21	36.0
22	144.0
23	340.0
24	925.0
25	2039.0
26	4204.0
27	7570.0
28	12652.0
29	19386.0
30	27813.0
31	38822.0
32	55857.0
33	88209.0
34	181011.0
35	602318.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.906382651787254	18.450627124829534	12.336208055624915	26.306782167758293
2	15.35381917669304	20.776079213549504	37.881396704429015	25.988704905328436
3	17.830346948202166	24.922191375126403	29.274864909246478	27.972596767424957
4	12.198958460372806	16.564666973321952	36.46656289042131	34.76981167588393
5	13.789980822841274	37.47958165561203	33.85512746520599	14.875310056340707
6	33.479939308967296	36.30879443793574	16.96679278621776	13.244473466879214
7	29.35382482044821	30.975960850555484	21.298553886663665	18.37166044233264
8	27.550576515134782	33.87462152239944	19.62033643093582	18.95446553152996
9	27.501742956630633	14.029120339871742	18.663848486652697	39.80528821684493
10	15.687185859676145	27.531128341147028	32.256509438854394	24.525176360322433
11	36.64771035836449	21.061284381115904	23.111715459474407	19.17928980104519
12	24.743167950586837	23.89445542386651	29.414063775397516	21.948312850149136
13	29.670357107531746	20.1004668028375	25.327294953900697	24.901881135730054
14	23.15792809463951	20.042426064084676	25.881723662085715	30.9179221791901
15	24.674900628735124	28.11695414468687	22.543520244464354	24.664624982113654
16	24.885603356467143	25.94069332288519	24.77151881290615	24.40218450774152
17	23.423118031234665	26.287784140288075	25.89819108593422	24.39090674254304
18	24.12048333923919	25.284945632498157	27.12680500778325	23.467766020479402
19	25.418714102846035	24.90783536260387	25.847391301425176	23.826059233124916
20	25.67209528556187	24.762972075215135	25.62772907417704	23.937203565045955
21	26.2607037258617	24.404062830402662	24.947015536667553	24.388217907068086
22	25.502092492187966	25.02453564727054	25.384647887053436	24.088723973488058
23	24.02749928937442	24.92941759432114	26.01244939193498	25.030633724369466
24	24.778242669788284	25.16966104282843	25.866262828410676	24.185833458972613
25	24.666986132064693	24.843972523414926	26.086238371763613	24.40280297275677
26	24.020616675613876	25.77209296518847	26.370195516535695	23.83709484266196
27	24.532473107540067	25.43367422885553	25.573502535828602	24.460350127775797
28	23.87332708470982	25.465381601389037	26.09104423718136	24.57024707671978
29	24.23495698703753	25.177900229901834	25.759569159734717	24.827573623325915
30	23.99381548242118	25.45485974397634	26.09357443988822	24.457750333714266
31	24.78331894395055	25.03061145113164	25.4905274965259	24.69554210839191
32	24.36550709495018	25.552864485299203	25.30884352696235	24.772784892788273
33	23.94326295622278	25.225608497111768	25.988312629946364	24.842815916719086
34	24.631475232254633	25.251269069656225	26.0751319966888	24.042123701400346
35	24.771586668382447	25.229853819309067	26.040080129528654	23.958479382779835
36	23.965968284700136	25.586882865440465	25.84445443230535	24.602694417554048
37	24.70536942141776	25.41313583737314	25.30135291903229	24.58014182217681
38	24.198238689292882	25.598355853909165	25.748941196807746	24.454464259990203
39	24.882046140534353	25.110895354543423	25.406872390874074	24.600186114048153
40	25.200587333155994	25.184165820782724	25.751236174959207	23.864010671102076
41	24.42440125726611	25.284762322734792	26.11592930807149	24.174907111927606
42	25.010083355740793	25.844024891483887	25.569949679253256	23.575942073522068
43	24.146451313657835	25.299112960457258	25.894890087570786	24.659545638314114
44	23.93964623984589	25.689720904192438	25.507069345172074	24.8635635107896
45	24.096111334115662	25.786731250492156	25.54089273950529	24.57626467588689
46	24.219798834955935	25.26787754937417	25.55731398379387	24.955009631876035
47	24.55044909475213	25.38285651727572	25.702355766783796	24.36433862118836
48	24.66453539124013	25.78244665169204	25.018606245744575	24.534411711323255
49	24.372588448367587	25.757436077676687	25.198061731880593	24.67191374207513
50	24.177128518240735	26.19686687031785	24.988692269353503	24.637312342087903
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	98.0
1	67.5
2	37.0
3	94.0
4	151.0
5	187.5
6	224.0
7	201.0
8	178.0
9	274.5
10	371.0
11	552.5
12	734.0
13	1285.5
14	1837.0
15	2500.0
16	3163.0
17	3442.0
18	3721.0
19	3790.0
20	3859.0
21	4314.0
22	4769.0
23	5378.5
24	5988.0
25	6851.5
26	7715.0
27	9528.5
28	11342.0
29	13699.5
30	16057.0
31	18562.5
32	21068.0
33	24975.0
34	28882.0
35	33475.5
36	38069.0
37	41566.0
38	45063.0
39	47105.0
40	49147.0
41	51918.0
42	54689.0
43	55805.0
44	56921.0
45	61345.0
46	65769.0
47	68842.5
48	71916.0
49	73808.0
50	75700.0
51	74800.5
52	73901.0
53	73009.5
54	72118.0
55	71394.0
56	70670.0
57	68158.5
58	65647.0
59	59779.5
60	53912.0
61	47701.5
62	41491.0
63	35851.0
64	30211.0
65	25801.0
66	21391.0
67	18154.0
68	14917.0
69	13316.5
70	11716.0
71	9049.5
72	6383.0
73	5417.0
74	4451.0
75	3492.0
76	2533.0
77	2200.0
78	1867.0
79	1516.5
80	1166.0
81	909.0
82	652.0
83	546.5
84	441.0
85	322.0
86	203.0
87	145.5
88	88.0
89	61.0
90	34.0
91	31.5
92	29.0
93	20.0
94	11.0
95	15.5
96	20.0
97	16.5
98	13.0
99	11.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007970476586484952
2	5.761790303483098E-4
3	5.761790303483098E-4
4	0.0
5	0.0
6	2.880895151741549E-4
7	0.0
8	0.0
9	8.642685455224647E-4
10	8.642685455224647E-4
11	9.602983839138497E-5
12	9.602983839138497E-5
13	3.841193535655399E-4
14	1.9205967678276995E-4
15	0.0044173725660037085
16	9.602983839138497E-4
17	0.0
18	3.841193535655399E-4
19	0.0
20	9.602983839138497E-4
21	0.0
22	9.602983839138497E-5
23	6.722088687396949E-4
24	1.9205967678276995E-4
25	0.0019205967678276993
26	0.004801491919569249
27	0.0063379693338314076
28	0.0045134024043950935
29	0.002784865313350164
30	0.0031689846669157038
31	0.0063379693338314076
32	0.003745163697264014
33	0.0046094322427864785
34	0.002784865313350164
35	0.002784865313350164
36	0.007586357232919413
37	0.003745163697264014
38	0.007010178202571103
39	0.0044173725660037085
40	0.002496775798176009
41	0.003457074182089859
42	0.0022086862830018542
43	0.0013444177374793897
44	0.0015364774142621596
45	8.642685455224647E-4
46	8.642685455224647E-4
47	0.002496775798176009
48	0.002496775798176009
49	9.602983839138497E-5
50	0.0019205967678276993
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1041343.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.267755684915514
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.31495105370624	50.81346701111834
2	12.357601102514707	15.636753748110863
3	3.4466242823321407	6.541805490968613
4	1.373649085209965	3.4763077887948706
5	0.7231724136386133	2.2876747792079226
6	0.4134551922012931	1.5695029252110722
7	0.2607435034666163	1.1547659391628358
8	0.19480842180020955	0.9860073308655702
9	0.13274211741243644	0.7558466268193559
>10	0.6751351206408681	7.539171124472807
>50	0.05754770822729311	2.636549958903562
>100	0.04696910468941463	5.59440696016539
>500	0.002600894160236838	1.007740316198804
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.9205967678276995E-4
13	0.0	0.0	0.0	0.0	1.9205967678276995E-4
14	0.0	0.0	0.0	9.602983839138497E-5	3.841193535655399E-4
15	0.0	0.0	0.0	9.602983839138497E-5	6.722088687396948E-4
16	0.0	0.0	0.0	1.9205967678276995E-4	6.722088687396948E-4
17	0.0	0.0	0.0	1.9205967678276995E-4	6.722088687396948E-4
18	0.0	0.0	0.0	1.9205967678276995E-4	6.722088687396948E-4
19	0.0	0.0	0.0	1.9205967678276995E-4	6.722088687396948E-4
20	0.0	0.0	0.0	3.841193535655399E-4	7.682387071310798E-4
21	0.0	0.0	0.0	3.841193535655399E-4	8.642685455224647E-4
22	0.0	0.0	0.0	7.682387071310798E-4	8.642685455224647E-4
23	0.0	0.0	0.0	0.0018245669294363145	9.602983839138497E-4
24	0.0	0.0	0.0	0.003072954828524319	0.0010563282223052346
25	0.0	0.0	0.0	0.004033253212438169	0.0010563282223052346
26	0.0	0.0	0.0	0.004705462081177864	0.0011523580606966196
27	0.0	0.0	0.0	0.005377670949917558	0.0011523580606966196
28	0.0	0.0	0.0	0.009987103192704037	0.0011523580606966196
29	0.0	0.0	0.0	0.018437728971145914	0.0011523580606966196
30	0.0	0.0	0.0	0.03265014505307089	0.0011523580606966196
31	0.0	0.0	0.0	0.06472411107579347	0.0011523580606966196
32	0.0	0.0	0.0	0.10006309160382314	0.0011523580606966196
33	0.0	0.0	0.0	0.13569016164702696	0.0011523580606966196
34	0.0	0.0	0.0	0.16949266476079447	0.0011523580606966196
35	0.0	0.0	0.0	0.20982519688517617	0.0011523580606966196
36	0.0	0.0	0.0	0.26801927895035543	0.0011523580606966196
37	0.0	0.0	0.0	0.3531977456035139	0.0011523580606966196
38	0.0	0.0	0.0	0.47640402825966083	0.0011523580606966196
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATAGG	120	5.984475E-10	22.000576	1
GTATCAA	2255	0.0	20.390778	1
TTAACGG	110	1.2642158E-7	20.001486	35
TATAGGA	200	0.0	18.698694	2
CGATTAA	60	0.00741031	18.333813	41
ACGATTA	60	0.0074123633	18.332932	40
CCGAAAT	60	0.0074144164	18.332052	12
GTCCTAT	305	0.0	18.03326	1
GGCGTTA	200	3.6379788E-12	17.601305	42
GTCCTAA	340	0.0	17.471046	1
GATATAC	270	0.0	17.111559	1
CTAGATA	180	2.8558134E-10	17.109917	3
GTATTAG	245	0.0	17.061672	1
GTATATA	155	1.4379111E-8	17.032703	1
AACGCAG	2685	0.0	16.877777	6
ATACCGT	185	4.2200554E-10	16.647486	6
GTCCTAC	645	0.0	16.031427	1
TAGGACG	675	0.0	15.969254	4
TAGGACA	280	0.0	15.713188	4
TACCGTC	210	1.5097612E-10	15.713188	7
>>END_MODULE