FastQCFastQC Report
Thu 2 Feb 2017
SRR4064053_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064053_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1439764
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30330.21065952475544603No Hit
TATCAACGCAGAGTACTTTTTTTTT19620.13627233352132712No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCGG250.006032121418.99985910
TCGACTG456.769404E-414.7756155
GTCGATA400.005276671614.2508879
AGTATCG400.005276671614.2508878
TCGCGTA801.2872079E-714.2508879
CGCGTAA903.6383426E-813.72212210
GCGTAAC852.697634E-713.41213211
CGCGGTC1153.765308E-1013.21729510
GTAATAC957.4094714E-812.9967433
AACCGCG1155.316906E-912.3920747
GTCTTAG1251.4169927E-912.1556861
TGATTAG550.003071619412.0891415
AGAACCG1353.7289283E-1011.9612125
CGACCAT1750.011.94276910
GTATCAA18150.011.8773281
CTAGGAC1052.7279748E-711.7589583
TTGCGCG907.4455584E-611.61304418
TAAGACT752.0771018E-411.3979354
GCGTAGG752.0804566E-411.3959551
GTTCAAA2800.011.1924561
Produced by FastQC (version 0.11.5)