##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064053_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1439764
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.195561216977225	32.0	32.0	32.0	32.0	32.0
2	30.766440194365188	32.0	32.0	32.0	32.0	32.0
3	30.781375280948822	32.0	32.0	32.0	32.0	32.0
4	30.820781739229485	32.0	32.0	32.0	32.0	32.0
5	30.69310317524261	32.0	32.0	32.0	32.0	32.0
6	34.35745024879078	36.0	36.0	36.0	32.0	36.0
7	34.298539205036384	36.0	36.0	36.0	32.0	36.0
8	34.23979763350105	36.0	36.0	36.0	32.0	36.0
9	34.407439691505	36.0	36.0	36.0	32.0	36.0
10	34.06582050947239	36.0	36.0	36.0	32.0	36.0
11	34.38899152916728	36.0	36.0	36.0	32.0	36.0
12	34.18376275556272	36.0	36.0	36.0	32.0	36.0
13	34.27153755754415	36.0	36.0	36.0	32.0	36.0
14	34.16027696205767	36.0	36.0	36.0	32.0	36.0
15	34.09598517534818	36.0	36.0	36.0	32.0	36.0
16	34.099573263395946	36.0	36.0	36.0	32.0	36.0
17	34.024380384563024	36.0	36.0	36.0	32.0	36.0
18	34.02050891673913	36.0	36.0	36.0	32.0	36.0
19	34.04563178409795	36.0	36.0	36.0	32.0	36.0
20	34.01531709363479	36.0	36.0	36.0	32.0	36.0
21	34.007024762391616	36.0	36.0	36.0	32.0	36.0
22	33.97926813005465	36.0	36.0	36.0	32.0	36.0
23	33.92774024076168	36.0	36.0	36.0	32.0	36.0
24	33.90920803687271	36.0	36.0	36.0	32.0	36.0
25	33.482761758177034	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	9.0
5	20.0
6	52.0
7	22.0
8	85.0
9	99.0
10	134.0
11	36.0
12	91.0
13	76.0
14	141.0
15	229.0
16	392.0
17	513.0
18	706.0
19	926.0
20	1416.0
21	2212.0
22	3610.0
23	5535.0
24	8385.0
25	12211.0
26	17347.0
27	22994.0
28	31364.0
29	42090.0
30	55171.0
31	74347.0
32	103291.0
33	144610.0
34	299406.0
35	612238.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.15035602519135	16.70323730010061	10.955278677954231	26.191127996753806
2	17.149594704415534	19.085705950586586	37.208357354708305	26.556341990289578
3	19.186199155679713	23.249785724902726	28.067884573443223	29.496130545974335
4	12.704953900566363	14.864757861630851	35.37897110120482	37.05131713659796
5	14.697395730587454	36.519300712124455	33.71595319928093	15.067350358007161
6	35.224186245538576	34.83024697790022	16.48286075903643	13.462706017524775
7	30.846831900803938	30.03024846256666	20.3628981834249	18.760021453204505
8	28.425865129739066	33.03095047021508	18.971467480988164	19.571716919057693
9	27.595405896340814	13.885711664959535	17.939810793419603	40.57907164528005
10	15.563226410845882	26.84471174294125	31.985775482235333	25.606286363977524
11	37.55833145120659	20.801264789172848	22.085164787435502	19.555238972185062
12	24.780204581752777	23.302414865328828	28.922227519706873	22.995153033211523
13	29.931782245312853	19.425552987784748	24.74306267484492	25.899602092057478
14	23.54128034829121	19.10979735834684	25.23582217353954	32.113100119822406
15	25.34483201970409	26.99138015510578	22.099480918959003	25.564306906231128
16	25.902313174432617	25.53005903522514	23.51970597761193	25.047921812730312
17	24.34027908088731	25.67334275742147	24.96153220661271	25.02484595507851
18	24.818298091226314	25.074376833354183	25.709221337114734	24.398103738304762
19	25.711419976200272	24.88544991492154	25.074791196325503	24.328338912552688
20	25.90932566673224	24.332607406676253	24.916986475242613	24.841080451348894
21	26.698283436019484	24.00930570074957	24.452487266175847	24.839923597055094
22	25.97339135149413	24.196770538637455	24.693493114977443	25.136344994890976
23	24.792934576710657	24.19657225479735	25.18562029258527	25.824872875906724
24	24.933356966847047	24.657122897746824	25.024206973971857	25.385313161434276
25	25.114744025073733	24.322257803740623	25.142965181626774	25.420032989558866
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	83.0
1	83.0
2	274.0
3	465.0
4	465.0
5	465.0
6	1265.0
7	2065.0
8	2065.0
9	2065.0
10	2356.0
11	2647.0
12	2647.0
13	2647.0
14	3121.0
15	3595.0
16	3595.0
17	3595.0
18	6132.5
19	8670.0
20	8670.0
21	8670.0
22	13488.0
23	18306.0
24	18306.0
25	18306.0
26	27533.0
27	36760.0
28	36760.0
29	36760.0
30	46935.0
31	57110.0
32	57110.0
33	57110.0
34	70506.0
35	83902.0
36	83902.0
37	83902.0
38	98241.5
39	112581.0
40	112581.0
41	112581.0
42	129416.5
43	146252.0
44	146252.0
45	146252.0
46	165831.5
47	185411.0
48	185411.0
49	185411.0
50	191598.0
51	197785.0
52	197785.0
53	197785.0
54	188492.5
55	179200.0
56	179200.0
57	179200.0
58	164796.0
59	150392.0
60	150392.0
61	150392.0
62	131560.0
63	112728.0
64	112728.0
65	112728.0
66	91760.0
67	70792.0
68	70792.0
69	70792.0
70	53177.5
71	35563.0
72	35563.0
73	35563.0
74	26781.0
75	17999.0
76	17999.0
77	17999.0
78	13902.5
79	9806.0
80	9806.0
81	9806.0
82	6826.0
83	3846.0
84	3846.0
85	3846.0
86	2891.5
87	1937.0
88	1937.0
89	1937.0
90	1350.5
91	764.0
92	764.0
93	764.0
94	547.5
95	331.0
96	331.0
97	331.0
98	552.5
99	774.0
100	774.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03750614684073223
2	0.005139731233729973
3	0.001805851514553774
4	0.003472791374141873
5	0.008890345917803195
6	0.014030077151533168
7	0.02444845127395879
8	0.036742132738421016
9	0.0487579908929519
10	0.05834289508558347
11	0.05480064788395876
12	0.0643160962491075
13	0.06869181338052625
14	0.06723324100338667
15	0.06077384904748278
16	0.06681650603848964
17	0.06237133307958804
18	0.07945746664036606
19	0.07584576361125851
20	0.07938801081288323
21	0.07473447037153312
22	0.07876290836553769
23	0.08397209542675049
24	0.07841562922812349
25	0.07827671757315782
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1439764.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.83729224943232
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.94721242736557	45.082557051901254
2	13.184298625678323	15.25088265434292
3	3.901629116214177	6.769789903301211
4	1.6464658195064459	3.8090849912598155
5	0.88198119238087	2.5505701991117586
6	0.5301555622959192	1.839765731650272
7	0.3603701675297744	1.4589984288177515
8	0.2513144174996987	1.1628276329140737
9	0.1996460416047759	1.0392287809263971
>10	0.9780028294605059	10.094351876632443
>50	0.06028679322911644	2.4440421974052478
>100	0.05331742112305675	6.014580416389478
>500	0.004715087690022825	1.8885372026655256
>1k	6.04498421797798E-4	0.5947829326819076
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3033	0.21065952475544603	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1962	0.13627233352132712	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.945582748283746E-5
2	0.0	0.0	0.0	0.0	6.945582748283746E-5
3	0.0	0.0	0.0	0.0	6.945582748283746E-5
4	0.0	0.0	0.0	0.0	6.945582748283746E-5
5	0.0	0.0	0.0	0.0	6.945582748283746E-5
6	0.0	0.0	0.0	0.0	6.945582748283746E-5
7	0.0	0.0	0.0	0.0	6.945582748283746E-5
8	0.0	0.0	0.0	0.0	6.945582748283746E-5
9	0.0	0.0	0.0	0.0	6.945582748283746E-5
10	0.0	0.0	0.0	0.0	6.945582748283746E-5
11	0.0	0.0	0.0	0.0	6.945582748283746E-5
12	0.0	0.0	0.0	0.0	1.3891165496567493E-4
13	0.0	0.0	0.0	6.945582748283746E-5	2.083674824485124E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGCGG	25	0.0060321214	18.999859	10
TCGACTG	45	6.769404E-4	14.775615	5
GTCGATA	40	0.0052766716	14.250887	9
AGTATCG	40	0.0052766716	14.250887	8
TCGCGTA	80	1.2872079E-7	14.250887	9
CGCGTAA	90	3.6383426E-8	13.722122	10
GCGTAAC	85	2.697634E-7	13.412132	11
CGCGGTC	115	3.765308E-10	13.217295	10
GTAATAC	95	7.4094714E-8	12.996743	3
AACCGCG	115	5.316906E-9	12.392074	7
GTCTTAG	125	1.4169927E-9	12.155686	1
TGATTAG	55	0.0030716194	12.089141	5
AGAACCG	135	3.7289283E-10	11.961212	5
CGACCAT	175	0.0	11.942769	10
GTATCAA	1815	0.0	11.877328	1
CTAGGAC	105	2.7279748E-7	11.758958	3
TTGCGCG	90	7.4455584E-6	11.613044	18
TAAGACT	75	2.0771018E-4	11.397935	4
GCGTAGG	75	2.0804566E-4	11.395955	1
GTTCAAA	280	0.0	11.192456	1
>>END_MODULE