FastQCFastQC Report
Thu 2 Feb 2017
SRR4064053_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064053_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1439764
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA450.00139820224.4448687
GTATCAA22150.021.8506371
CGTATAT2050.020.39059610
GTCCTAT3400.018.764381
ACCGTAT600.007412028518.3336528
TAGCGTA2350.017.7875427
TAACGGC1408.3702616E-817.28661536
TTAACGG1408.3702616E-817.28661535
AGCGTAT2450.017.061528
TAGGACG2450.017.061524
GTATTAC1551.439912E-817.0319611
AACGCAG28100.016.9896186
ATAGCGT2600.016.923376
GTATTAG3400.016.8232361
AAATTCG800.001988830316.49971221
GATATAA1906.166374E-1016.2102451
CGTTTAT3400.016.1767531
GTACCGT1105.621604E-516.0002786
TTAGACC1105.621604E-516.0002784
GGTATAT1105.623274E-515.9997231